Zinc in PDB 5zgp: Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin

Enzymatic activity of Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin

All present enzymatic activity of Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin:
3.5.2.6;

Protein crystallography data

The structure of Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin, PDB code: 5zgp was solved by H.Zhang, Q.Hao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 1.15
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 39.240, 79.130, 134.442, 90.00, 90.00, 90.00
R / Rfree (%) 13.2 / 15.7

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin (pdb code 5zgp). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin, PDB code: 5zgp:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in 5zgp

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Zinc binding site 1 out of 4 in the Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn301

b:10.1
occ:1.00
O A:HOH405 1.9 10.2 1.0
ND1 A:HIS122 2.0 10.8 1.0
NE2 A:HIS189 2.0 10.3 1.0
NE2 A:HIS120 2.1 10.3 1.0
O4 A:ZZ7304 2.2 11.3 1.0
CE1 A:HIS122 2.9 10.9 1.0
CD2 A:HIS189 3.0 9.5 1.0
CG A:HIS122 3.0 10.0 1.0
CE1 A:HIS189 3.0 10.1 1.0
CE1 A:HIS120 3.1 9.7 1.0
CD2 A:HIS120 3.2 9.5 1.0
C15 A:ZZ7304 3.2 11.3 1.0
CB A:HIS122 3.4 9.7 1.0
C14 A:ZZ7304 3.9 10.7 1.0
N3 A:ZZ7304 4.0 10.7 1.0
OXT A:ZZ7304 4.1 12.6 1.0
NE2 A:HIS122 4.1 11.3 1.0
CD2 A:HIS122 4.1 10.6 1.0
ND1 A:HIS189 4.2 10.0 1.0
CG A:HIS189 4.2 9.6 1.0
OD1 A:ASP124 4.2 10.7 1.0
ND1 A:HIS120 4.2 9.5 1.0
CG2 A:THR190 4.3 9.4 1.0
CG A:HIS120 4.3 9.1 1.0
SG A:CYS208 4.4 10.0 1.0
CB A:CYS208 4.5 9.6 1.0
C13 A:ZZ7304 4.5 10.6 1.0
ZN A:ZN302 4.6 10.0 1.0
O A:HOH553 4.8 18.6 1.0
CA A:HIS122 4.9 9.4 1.0
C12 A:ZZ7304 4.9 11.0 1.0

Zinc binding site 2 out of 4 in 5zgp

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Zinc binding site 2 out of 4 in the Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn302

b:10.0
occ:1.00
NE2 A:HIS250 2.0 11.1 1.0
OD2 A:ASP124 2.1 11.3 1.0
O1 A:ZZ7304 2.1 10.8 1.0
N3 A:ZZ7304 2.2 10.7 1.0
SG A:CYS208 2.4 10.0 1.0
C2 A:ZZ7304 3.0 11.2 1.0
CD2 A:HIS250 3.0 11.2 1.0
CE1 A:HIS250 3.0 11.2 1.0
O A:HOH405 3.0 10.2 1.0
C12 A:ZZ7304 3.1 11.0 1.0
C13 A:ZZ7304 3.3 10.6 1.0
CG A:ASP124 3.3 10.5 1.0
CB A:CYS208 3.4 9.6 1.0
C16 A:ZZ7304 3.6 12.6 1.0
OD1 A:ASP124 3.8 10.7 1.0
C6 A:ZZ7304 3.9 11.5 1.0
ND1 A:HIS250 4.1 11.2 1.0
CG A:HIS250 4.1 11.4 1.0
O2 A:ZZ7304 4.2 12.7 1.0
CB A:SER249 4.2 10.0 1.0
O4 A:ZZ7304 4.3 11.3 1.0
C14 A:ZZ7304 4.3 10.7 1.0
CB A:ASP124 4.5 10.2 1.0
CA A:CYS208 4.5 9.7 1.0
S1 A:ZZ7304 4.5 12.0 1.0
ZN A:ZN301 4.6 10.1 1.0
OG A:SER249 4.6 9.9 1.0
C15 A:ZZ7304 4.8 11.3 1.0
NE2 A:HIS189 4.8 10.3 1.0
CE1 A:HIS189 4.9 10.1 1.0

Zinc binding site 3 out of 4 in 5zgp

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Zinc binding site 3 out of 4 in the Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn301

b:10.4
occ:1.00
O B:HOH409 2.0 10.6 1.0
ND1 B:HIS122 2.0 10.8 1.0
NE2 B:HIS189 2.0 10.9 1.0
NE2 B:HIS120 2.1 11.1 1.0
O4 B:ZZ7303 2.2 11.5 1.0
CE1 B:HIS122 2.9 10.9 1.0
CG B:HIS122 3.0 10.7 1.0
CD2 B:HIS189 3.0 9.7 1.0
CE1 B:HIS189 3.0 10.5 1.0
CE1 B:HIS120 3.1 10.7 1.0
CD2 B:HIS120 3.2 9.8 1.0
C15 B:ZZ7303 3.2 11.9 1.0
CB B:HIS122 3.4 10.1 1.0
C14 B:ZZ7303 3.8 11.4 1.0
N3 B:ZZ7303 4.1 10.8 1.0
NE2 B:HIS122 4.1 11.4 1.0
OXT B:ZZ7303 4.1 13.5 1.0
CD2 B:HIS122 4.1 11.2 1.0
ND1 B:HIS189 4.1 10.2 1.0
CG B:HIS189 4.2 9.7 1.0
ND1 B:HIS120 4.2 10.4 1.0
OD1 B:ASP124 4.2 10.6 1.0
CG2 B:THR190 4.2 10.0 1.0
CG B:HIS120 4.3 9.5 1.0
SG B:CYS208 4.4 10.7 1.0
CB B:CYS208 4.5 10.2 1.0
C13 B:ZZ7303 4.6 11.7 1.0
ZN B:ZN302 4.6 10.7 1.0
O B:HOH526 4.7 16.8 1.0
CA B:HIS122 4.9 10.0 1.0
C12 B:ZZ7303 4.9 11.7 1.0

Zinc binding site 4 out of 4 in 5zgp

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Zinc binding site 4 out of 4 in the Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of Ndm-1 at PH6.2 (Bis-Tris) in Complex with Hydrolyzed Ampicillin within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn302

b:10.7
occ:1.00
NE2 B:HIS250 2.0 11.9 1.0
OD2 B:ASP124 2.1 11.9 1.0
O1 B:ZZ7303 2.1 11.6 1.0
N3 B:ZZ7303 2.2 10.8 1.0
SG B:CYS208 2.4 10.7 1.0
C2 B:ZZ7303 3.0 11.2 1.0
O B:HOH409 3.0 10.6 1.0
CD2 B:HIS250 3.0 12.1 1.0
C12 B:ZZ7303 3.0 11.7 1.0
CE1 B:HIS250 3.0 11.7 1.0
CG B:ASP124 3.3 11.2 1.0
C13 B:ZZ7303 3.3 11.7 1.0
CB B:CYS208 3.4 10.2 1.0
C16 B:ZZ7303 3.6 13.0 1.0
OD1 B:ASP124 3.8 10.6 1.0
C6 B:ZZ7303 3.9 12.3 1.0
ND1 B:HIS250 4.1 12.0 1.0
CG B:HIS250 4.2 12.4 1.0
O2 B:ZZ7303 4.2 13.2 1.0
CB B:SER249 4.2 11.4 1.0
C14 B:ZZ7303 4.3 11.4 1.0
O4 B:ZZ7303 4.3 11.5 1.0
CB B:ASP124 4.5 11.3 1.0
S1 B:ZZ7303 4.5 13.1 1.0
CA B:CYS208 4.5 10.6 1.0
ZN B:ZN301 4.6 10.4 1.0
OG B:SER249 4.6 11.1 1.0
C15 B:ZZ7303 4.8 11.9 1.0
NE2 B:HIS189 4.8 10.9 1.0
CE1 B:HIS189 4.9 10.5 1.0

Reference:

H.Zhang, G.Ma, Y.Zhu, L.Zeng, A.Ahmad, C.Wang, B.Pang, H.Fang, L.Zhao, Q.Hao. Active-Site Conformational Fluctuations Promote the Enzymatic Activity of Ndm-1. Antimicrob. Agents V. 62 2018CHEMOTHER..
ISSN: ESSN 1098-6596
PubMed: 30150473
DOI: 10.1128/AAC.01579-18
Page generated: Wed Dec 16 11:26:30 2020

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