Zinc in PDB 5ze1: Hairpin Forming Complex, RAG1/2-Nicked 12RSS/23RSS Complex in 2MM MN2+ For 10 Min at 4'C

All present enzymatic activity of Hairpin Forming Complex, RAG1/2-Nicked 12RSS/23RSS Complex in 2MM MN2+ For 10 Min at 4'C:
2.3.2.27;

The structure of Hairpin Forming Complex, RAG1/2-Nicked 12RSS/23RSS Complex in 2MM MN2+ For 10 Min at 4'C, PDB code: 5ze1 was solved by M.S.Kim, W.Chuenchor, X.Chen, M.Gellert, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.68 / 3.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	132.760, 108.600, 157.090, 90.00, 114.20, 90.00
R / Rfree (%)	19.7 / 23.2

The structure of Hairpin Forming Complex, RAG1/2-Nicked 12RSS/23RSS Complex in 2MM MN2+ For 10 Min at 4'C also contains other interesting chemical elements:

Potassium	(K)	2 atoms
Manganese	(Mn)	4 atoms

The binding sites of Zinc atom in the Hairpin Forming Complex, RAG1/2-Nicked 12RSS/23RSS Complex in 2MM MN2+ For 10 Min at 4'C (pdb code 5ze1). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Hairpin Forming Complex, RAG1/2-Nicked 12RSS/23RSS Complex in 2MM MN2+ For 10 Min at 4'C, PDB code: 5ze1:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Hairpin Forming Complex, RAG1/2-Nicked 12RSS/23RSS Complex in 2MM MN2+ For 10 Min at 4'C


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Hairpin Forming Complex, RAG1/2-Nicked 12RSS/23RSS Complex in 2MM MN2+ For 10 Min at 4'C within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2001 b:66.5 occ:1.00 NE2 A:HIS942 1.9 65.2 1.0 SG A:CYS730 2.1 61.6 1.0 NE2 A:HIS937 2.2 68.3 1.0 SG A:CYS727 2.2 63.6 1.0 CE1 A:HIS942 2.8 68.1 1.0 CB A:CYS727 2.8 58.1 1.0 CD2 A:HIS937 2.8 67.6 1.0 CD2 A:HIS942 3.0 64.8 1.0 CB A:CYS730 3.4 63.5 1.0 CE1 A:HIS937 3.4 76.0 1.0 ND1 A:HIS942 3.9 65.6 1.0 N A:CYS730 3.9 60.2 1.0 CG A:HIS942 4.0 64.4 1.0 CG2 A:THR732 4.1 68.5 1.0 CG A:HIS937 4.1 68.3 1.0 CA A:CYS730 4.2 65.6 1.0 CA A:CYS727 4.3 65.0 1.0 ND1 A:HIS937 4.3 69.5 1.0 C A:LEU729 4.6 64.4 1.0 CB A:LEU729 4.7 60.8 1.0 CB A:HIS744 4.7 77.6 1.0 C A:CYS730 4.8 67.1 1.0 N A:LEU729 5.0 67.5 1.0 CA A:LEU729 5.0 65.0 1.0

Zinc binding site 2 out of 2 in the Hairpin Forming Complex, RAG1/2-Nicked 12RSS/23RSS Complex in 2MM MN2+ For 10 Min at 4'C


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Hairpin Forming Complex, RAG1/2-Nicked 12RSS/23RSS Complex in 2MM MN2+ For 10 Min at 4'C within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn2001 b:71.6 occ:1.00 NE2 C:HIS942 1.7 59.4 1.0 SG C:CYS730 2.0 58.9 1.0 NE2 C:HIS937 2.1 61.7 1.0 SG C:CYS727 2.3 59.2 1.0 CD2 C:HIS942 2.7 59.5 1.0 CD2 C:HIS937 2.8 57.8 1.0 CE1 C:HIS942 2.8 65.1 1.0 CB C:CYS730 3.2 57.8 1.0 CE1 C:HIS937 3.2 69.1 1.0 CB C:CYS727 3.3 54.7 1.0 N C:CYS730 3.5 67.7 1.0 CG C:HIS942 3.8 58.9 1.0 ND1 C:HIS942 3.8 65.2 1.0 CA C:CYS730 3.9 64.2 1.0 CG C:HIS937 4.0 55.8 1.0 ND1 C:HIS937 4.2 65.8 1.0 CB C:LEU729 4.3 65.5 1.0 C C:LEU729 4.4 65.8 1.0 C C:CYS730 4.6 63.9 1.0 CA C:CYS727 4.7 58.0 1.0 CB C:ALA941 4.7 58.8 1.0 CG2 C:THR732 4.8 62.5 1.0 CA C:LEU729 4.8 65.1 1.0 O C:CYS730 4.8 65.3 1.0 N C:LEU729 4.9 62.8 1.0 CB C:HIS744 4.9 74.8 1.0

M.S.Kim, W.Chuenchor, X.Chen, Y.Cui, X.Zhang, Z.H.Zhou, M.Gellert, W.Yang. Cracking the Dna Code For V(D)J Recombination Mol. Cell V. 70 358 2018.
ISSN: ISSN 1097-4164
PubMed: 29628308
DOI: 10.1016/J.MOLCEL.2018.03.008