Zinc in PDB 5z4g: Crystal Structure of Secretory Abundant Heat Soluble Protein 4 From Ramazzottius Varieornatus

The structure of Crystal Structure of Secretory Abundant Heat Soluble Protein 4 From Ramazzottius Varieornatus, PDB code: 5z4g was solved by Y.Fukuda, T.Inoue, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.01 / 1.50
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	32.802, 41.926, 53.483, 89.97, 80.75, 78.76
R / Rfree (%)	15.9 / 20.3

The binding sites of Zinc atom in the Crystal Structure of Secretory Abundant Heat Soluble Protein 4 From Ramazzottius Varieornatus (pdb code 5z4g). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structure of Secretory Abundant Heat Soluble Protein 4 From Ramazzottius Varieornatus, PDB code: 5z4g:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Crystal Structure of Secretory Abundant Heat Soluble Protein 4 From Ramazzottius Varieornatus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Secretory Abundant Heat Soluble Protein 4 From Ramazzottius Varieornatus within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn201 b:30.2 occ:1.00 NE2 A:HIS85 2.1 21.6 1.0 O A:HOH356 2.1 41.3 1.0 NE2 A:HIS83 2.1 24.5 1.0 OE2 A:GLU78 2.3 40.3 1.0 O A:HOH380 2.3 51.1 1.0 OE2 A:GLU81 2.3 36.7 1.0 CE1 A:HIS85 3.0 21.7 1.0 CE1 A:HIS83 3.1 25.3 1.0 CD2 A:HIS85 3.1 19.7 1.0 CD2 A:HIS83 3.2 21.6 1.0 CD A:GLU81 3.4 37.5 1.0 CD A:GLU78 3.5 44.2 1.0 O A:HOH308 4.1 19.1 1.0 ND1 A:HIS85 4.1 20.7 1.0 OE1 A:GLU81 4.2 43.8 1.0 ND1 A:HIS83 4.2 26.0 1.0 OE1 A:GLU78 4.2 48.9 1.0 CG A:HIS85 4.2 17.7 1.0 CG A:HIS83 4.3 22.1 1.0 CG A:GLU81 4.3 35.4 1.0 CG A:GLU78 4.5 41.6 1.0 CB A:GLU81 4.7 32.0 1.0

Zinc binding site 2 out of 4 in the Crystal Structure of Secretory Abundant Heat Soluble Protein 4 From Ramazzottius Varieornatus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Secretory Abundant Heat Soluble Protein 4 From Ramazzottius Varieornatus within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn202 b:42.3 occ:0.93 NE2 A:HIS111 2.1 28.5 1.0 ND1 A:HIS95 2.1 33.8 0.7 CE1 A:HIS95 2.9 34.0 0.7 CD2 A:HIS95 2.9 26.7 0.3 CD2 A:HIS111 3.0 26.9 1.0 CE1 A:HIS111 3.1 31.8 1.0 CG A:HIS95 3.3 33.6 0.7 CG A:HIS95 3.7 27.6 0.3 CB A:HIS95 3.8 34.3 0.7 NE2 A:HIS95 3.9 24.9 0.3 CB A:HIS95 4.0 29.5 0.3 NE2 A:HIS95 4.0 31.8 0.7 CA A:HIS95 4.1 29.0 0.3 CA A:HIS95 4.2 31.8 0.7 ND1 A:HIS111 4.2 32.9 1.0 CG A:HIS111 4.2 28.5 1.0 OD2 A:ASP112 4.2 32.8 1.0 CD2 A:HIS95 4.3 33.0 0.7 OD1 A:ASP112 4.5 28.6 1.0 CG A:ASP112 4.7 29.7 1.0 N A:HIS95 4.8 34.6 0.7 ND1 A:HIS95 4.9 27.5 0.3 N A:HIS95 4.9 32.1 0.3 O A:GLU94 5.0 44.3 1.0 CE1 A:HIS95 5.0 26.1 0.3

Zinc binding site 3 out of 4 in the Crystal Structure of Secretory Abundant Heat Soluble Protein 4 From Ramazzottius Varieornatus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Secretory Abundant Heat Soluble Protein 4 From Ramazzottius Varieornatus within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn201 b:31.1 occ:0.85 O B:HOH389 2.0 44.8 0.8 NE2 B:HIS85 2.0 20.4 1.0 NE2 B:HIS83 2.1 25.2 1.0 OE1 B:GLU81 2.2 35.4 1.0 O B:HOH393 2.3 41.8 0.8 OE2 B:GLU81 2.5 37.8 1.0 CD B:GLU81 2.7 35.5 1.0 CE1 B:HIS85 3.0 21.7 1.0 CD2 B:HIS85 3.0 20.3 1.0 CE1 B:HIS83 3.1 26.8 1.0 CD2 B:HIS83 3.2 23.1 1.0 ND1 B:HIS85 4.1 20.4 1.0 CG B:GLU81 4.1 34.3 1.0 CG B:HIS85 4.2 18.5 1.0 ND1 B:HIS83 4.2 26.4 1.0 O B:HOH312 4.2 21.4 1.0 CG B:HIS83 4.3 23.9 1.0 CB B:GLU81 4.7 32.1 1.0 CG B:GLU78 4.7 48.3 1.0

Zinc binding site 4 out of 4 in the Crystal Structure of Secretory Abundant Heat Soluble Protein 4 From Ramazzottius Varieornatus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of Secretory Abundant Heat Soluble Protein 4 From Ramazzottius Varieornatus within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn202 b:37.9 occ:0.95 NE2 B:HIS111 2.0 25.3 1.0 N A:SER27 2.0 33.1 1.0 O A:SER27 2.3 33.5 1.0 ND1 B:HIS95 2.3 21.1 0.4 CD2 B:HIS95 2.7 37.0 0.6 CE1 B:HIS111 2.9 29.4 1.0 C A:SER27 3.0 34.9 1.0 CA A:SER27 3.0 37.3 1.0 CD2 B:HIS111 3.0 23.9 1.0 CE1 B:HIS95 3.1 19.6 0.4 CG B:HIS95 3.3 34.6 0.6 NE2 B:HIS95 3.4 36.8 0.6 OG A:SER27 3.5 40.4 1.0 CG B:HIS95 3.5 21.2 0.4 CB A:SER27 3.8 38.8 1.0 CB B:HIS95 3.9 34.9 0.6 CB B:HIS95 3.9 26.1 0.4 ND1 B:HIS111 4.0 30.1 1.0 CG B:HIS111 4.1 25.3 1.0 CA B:HIS95 4.1 27.9 0.4 CA B:HIS95 4.1 32.2 0.6 ND1 B:HIS95 4.3 35.4 0.6 N A:GLN28 4.3 35.7 1.0 CE1 B:HIS95 4.3 35.9 0.6 NE2 B:HIS95 4.3 19.5 0.4 OD2 B:ASP112 4.4 36.0 1.0 CD2 B:HIS95 4.5 20.2 0.4 N B:HIS95 4.9 31.9 0.4 N B:HIS95 4.9 33.8 0.6 CG B:ASP112 5.0 28.9 1.0 OD1 B:ASP112 5.0 29.7 1.0

Y.Fukuda, T.Inoue. Crystal Structure of Secretory Abundant Heat Soluble Protein 4 From One of the Toughest "Water Bears" Micro-Animals Ramazzottius Varieornatus Protein Sci. V. 27 993 2018.
ISSN: ESSN 1469-896X
PubMed: 29493034
DOI: 10.1002/PRO.3393