Zinc in PDB 5yo1: Structure of Epepn E298A Mutant in Complex with Puromycin

All present enzymatic activity of Structure of Epepn E298A Mutant in Complex with Puromycin:
3.4.11.2;

The structure of Structure of Epepn E298A Mutant in Complex with Puromycin, PDB code: 5yo1 was solved by R.J.Ganji, R.Reddi, A.K.Marapaka, A.Addlagatta, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.90 / 2.50
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	120.325, 120.325, 170.012, 90.00, 90.00, 120.00
R / Rfree (%)	12.6 / 18.4

The structure of Structure of Epepn E298A Mutant in Complex with Puromycin also contains other interesting chemical elements:

Sodium

(Na)

1 atom

The binding sites of Zinc atom in the Structure of Epepn E298A Mutant in Complex with Puromycin (pdb code 5yo1). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Structure of Epepn E298A Mutant in Complex with Puromycin, PDB code: 5yo1:

Zinc binding site 1 out of 1 in the Structure of Epepn E298A Mutant in Complex with Puromycin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Epepn E298A Mutant in Complex with Puromycin within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn901 b:28.7 occ:1.00 OE1 A:GLU320 2.1 29.6 1.0 NE2 A:HIS297 2.2 25.6 1.0 NE2 A:HIS301 2.2 22.0 1.0 O A:HOH1645 2.2 3.8 1.0 O A:HOH1148 2.6 56.0 1.0 OE2 A:GLU320 2.7 25.2 1.0 CD A:GLU320 2.7 25.3 1.0 CD2 A:HIS297 3.0 24.8 1.0 CE1 A:HIS301 3.1 21.3 1.0 CD2 A:HIS301 3.2 21.2 1.0 CE1 A:HIS297 3.2 26.6 1.0 O A:HOH1485 3.7 55.6 1.0 CE2 A:TYR381 3.7 20.6 1.0 O A:HOH1206 3.8 21.7 1.0 OH A:TYR381 3.8 20.1 1.0 O A:HOH1038 4.0 18.4 1.0 CG A:GLU320 4.2 25.8 1.0 CZ A:TYR381 4.2 22.7 1.0 OE1 A:GLU264 4.2 19.4 1.0 CG A:HIS297 4.2 23.0 1.0 ND1 A:HIS301 4.2 22.8 1.0 ND1 A:HIS297 4.3 24.5 1.0 CG A:HIS301 4.3 21.3 1.0 CG2 A:THR323 4.7 21.3 1.0 CD2 A:TYR381 4.8 20.6 1.0 CD A:GLU264 4.8 19.3 1.0 CB A:GLU320 4.8 24.9 1.0 NZ A:LYS319 4.9 17.4 1.0 OE2 A:GLU264 4.9 18.6 1.0 CA A:GLU320 4.9 22.4 1.0 CB A:THR323 4.9 21.2 1.0

R.J.Ganji, R.Reddi, A.K.Marapaka, A.Addlagatta. Molecular Determinants of Selective Inhibition of Human Puromycin Sensitive Aminopeptidase By Puromycin To Be Published.