Zinc in PDB 5wfi: X-Ray Structure of Mhv PLP2 (CYS1716SER) Catalytic Mutant in Complex with Free Ubiquitin

The structure of X-Ray Structure of Mhv PLP2 (CYS1716SER) Catalytic Mutant in Complex with Free Ubiquitin, PDB code: 5wfi was solved by A.D.Mesecar, Y.Chen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.58 / 1.85
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	199.515, 43.388, 110.159, 90.00, 114.85, 90.00
R / Rfree (%)	15.7 / 19.7

The binding sites of Zinc atom in the X-Ray Structure of Mhv PLP2 (CYS1716SER) Catalytic Mutant in Complex with Free Ubiquitin (pdb code 5wfi). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the X-Ray Structure of Mhv PLP2 (CYS1716SER) Catalytic Mutant in Complex with Free Ubiquitin, PDB code: 5wfi:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the X-Ray Structure of Mhv PLP2 (CYS1716SER) Catalytic Mutant in Complex with Free Ubiquitin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of X-Ray Structure of Mhv PLP2 (CYS1716SER) Catalytic Mutant in Complex with Free Ubiquitin within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2005 b:27.9 occ:0.69 SG A:CYS1830 2.3 44.2 1.0 SG A:CYS1828 2.3 42.6 1.0 SG A:CYS1794 2.4 42.5 1.0 SG A:CYS1796 2.4 52.6 1.0 CB A:CYS1794 3.3 43.5 1.0 CB A:CYS1830 3.3 52.6 1.0 CB A:CYS1796 3.3 54.6 1.0 CB A:CYS1828 3.5 37.4 1.0 N A:CYS1796 3.6 44.3 1.0 N A:CYS1830 4.0 38.1 1.0 NZ A:LYS1833 4.0 53.6 1.0 CA A:CYS1796 4.1 56.2 1.0 CA A:CYS1830 4.2 39.6 1.0 N A:LYS1795 4.4 46.2 1.0 O A:SER1832 4.5 45.8 1.0 CA A:CYS1794 4.6 36.4 1.0 C A:CYS1794 4.7 44.1 1.0 C A:LYS1795 4.7 48.6 1.0 CA A:CYS1828 4.8 35.5 1.0 N A:THR1829 4.8 35.2 1.0 CB A:LYS1833 4.8 37.6 1.0 CG2 A:THR1829 4.8 46.2 1.0 CE A:LYS1833 4.9 49.9 1.0 C A:CYS1828 4.9 41.0 1.0 C A:CYS1830 5.0 43.0 1.0

Zinc binding site 2 out of 2 in the X-Ray Structure of Mhv PLP2 (CYS1716SER) Catalytic Mutant in Complex with Free Ubiquitin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of X-Ray Structure of Mhv PLP2 (CYS1716SER) Catalytic Mutant in Complex with Free Ubiquitin within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2005 b:28.1 occ:0.86 SG B:CYS1828 2.3 32.8 1.0 SG B:CYS1794 2.3 32.8 1.0 SG B:CYS1796 2.4 35.6 1.0 SG B:CYS1830 2.4 33.0 1.0 CB B:CYS1796 3.2 37.7 1.0 CB B:CYS1794 3.3 27.9 1.0 CB B:CYS1830 3.3 30.9 1.0 CB B:CYS1828 3.4 29.6 1.0 N B:CYS1796 3.6 32.5 1.0 N B:CYS1830 3.9 31.6 1.0 CA B:CYS1796 4.0 40.8 1.0 NZ B:LYS1833 4.1 35.1 1.0 CA B:CYS1830 4.2 32.4 1.0 O D:HOH289 4.2 43.2 1.0 N B:LYS1795 4.3 31.2 1.0 CA B:CYS1794 4.5 30.3 1.0 O B:SER1832 4.5 30.1 1.0 N B:THR1829 4.6 25.6 1.0 C B:CYS1794 4.7 35.7 1.0 C B:LYS1795 4.7 37.5 1.0 CA B:CYS1828 4.7 24.2 1.0 C B:CYS1828 4.8 26.5 1.0 CB B:LYS1833 4.9 24.1 1.0 CG2 B:THR1829 4.9 26.0 1.0 CA B:LYS1795 5.0 40.6 1.0 CE B:LYS1833 5.0 33.7 1.0 C B:CYS1830 5.0 34.0 1.0

A.D.Mesecar, Y.Chen. X-Ray Structure of Mhv PLP2 (CYS1716SER) Catalytic Mutant in Complex with Free Ubiquitin To Be Published.