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Zinc in PDB 5vi8: Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment

Enzymatic activity of Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment

All present enzymatic activity of Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment:
2.7.7.6;

Protein crystallography data

The structure of Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment, PDB code: 5vi8 was solved by E.A.Hubin, E.A.Campbell, S.A.Darst, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 51.99 / 2.76
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 133.012, 161.633, 139.211, 90.00, 107.72, 90.00
R / Rfree (%) 24 / 28.1

Other elements in 5vi8:

The structure of Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment also contains other interesting chemical elements:

Magnesium (Mg) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment (pdb code 5vi8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment, PDB code: 5vi8:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 5vi8

Go back to Zinc Binding Sites List in 5vi8
Zinc binding site 1 out of 2 in the Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2001

b:78.6
occ:1.00
SG D:CYS977 2.2 58.9 1.0
SG D:CYS967 2.2 61.6 1.0
SG D:CYS890 2.3 60.5 1.0
SG D:CYS974 2.3 46.8 1.0
CB D:CYS977 2.9 60.2 1.0
CB D:CYS967 3.2 59.9 1.0
CB D:CYS974 3.4 58.6 1.0
CB D:CYS890 3.4 63.2 1.0
NH1 D:ARG962 3.5 58.2 1.0
CA D:CYS967 3.6 58.8 1.0
N D:CYS890 4.0 70.2 1.0
OG D:SER969 4.1 68.0 1.0
N D:CYS974 4.1 46.0 1.0
CA D:CYS977 4.2 50.1 1.0
N D:THR968 4.2 60.4 1.0
N D:CYS977 4.2 45.7 1.0
CA D:CYS974 4.3 49.8 1.0
CA D:CYS890 4.3 65.1 1.0
C D:CYS967 4.3 57.4 1.0
CG2 D:THR892 4.5 63.4 1.0
O D:CYS974 4.6 41.9 1.0
CZ D:ARG962 4.6 45.2 1.0
N D:SER969 4.7 53.9 1.0
C D:CYS974 4.8 49.0 1.0
N D:CYS967 4.9 61.7 1.0
NH2 D:ARG962 4.9 64.6 1.0

Zinc binding site 2 out of 2 in 5vi8

Go back to Zinc Binding Sites List in 5vi8
Zinc binding site 2 out of 2 in the Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2002

b:0.8
occ:1.00
SG D:CYS62 2.1 82.8 1.0
SG D:CYS60 2.2 91.5 1.0
SG D:CYS75 2.2 67.8 1.0
SG D:CYS78 2.2 93.1 1.0
CB D:CYS75 2.9 81.6 1.0
CB D:CYS60 3.2 93.6 1.0
N D:CYS62 3.6 87.6 1.0
CB D:CYS62 3.6 0.8 1.0
CB D:CYS78 3.6 82.0 1.0
N D:GLY63 3.8 96.3 1.0
N D:CYS78 3.9 80.7 1.0
CA D:CYS62 4.0 98.1 1.0
N D:LYS64 4.1 92.9 1.0
N D:TYR61 4.3 84.1 1.0
C D:CYS62 4.3 90.4 1.0
C D:CYS60 4.4 85.8 1.0
CA D:CYS78 4.4 77.2 1.0
CA D:CYS75 4.4 66.0 1.0
CA D:CYS60 4.4 87.3 1.0
CB D:LYS64 4.6 96.2 1.0
C D:TYR61 4.7 85.5 1.0
CB D:ARG77 4.7 96.7 1.0
CA D:GLY63 4.8 97.8 1.0
C D:GLY63 4.9 91.2 1.0
O D:CYS60 4.9 89.8 1.0
CA D:LYS64 4.9 92.2 1.0
C D:CYS75 5.0 80.5 1.0
CA D:TYR61 5.0 91.1 1.0

Reference:

E.A.Hubin, M.Lilic, S.A.Darst, E.A.Campbell. Structural Insights Into the Mycobacteria Transcription Initiation Complex From Analysis of X-Ray Crystal Structures. Nat Commun V. 8 16072 2017.
ISSN: ESSN 2041-1723
PubMed: 28703128
DOI: 10.1038/NCOMMS16072
Page generated: Mon Oct 28 12:57:43 2024

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