Zinc in PDB 5vi8: Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment

Enzymatic activity of Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment

All present enzymatic activity of Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment:
2.7.7.6;

Protein crystallography data

The structure of Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment, PDB code: 5vi8 was solved by E.A.Hubin, E.A.Campbell, S.A.Darst, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 51.99 / 2.76
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 133.012, 161.633, 139.211, 90.00, 107.72, 90.00
R / Rfree (%) 24 / 28.1

Other elements in 5vi8:

The structure of Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment also contains other interesting chemical elements:

Magnesium (Mg) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment (pdb code 5vi8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment, PDB code: 5vi8:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 5vi8

Go back to Zinc Binding Sites List in 5vi8
Zinc binding site 1 out of 2 in the Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2001

b:78.6
occ:1.00
SG D:CYS977 2.2 58.9 1.0
SG D:CYS967 2.2 61.6 1.0
SG D:CYS890 2.3 60.5 1.0
SG D:CYS974 2.3 46.8 1.0
CB D:CYS977 2.9 60.2 1.0
CB D:CYS967 3.2 59.9 1.0
CB D:CYS974 3.4 58.6 1.0
CB D:CYS890 3.4 63.2 1.0
NH1 D:ARG962 3.5 58.2 1.0
CA D:CYS967 3.6 58.8 1.0
N D:CYS890 4.0 70.2 1.0
OG D:SER969 4.1 68.0 1.0
N D:CYS974 4.1 46.0 1.0
CA D:CYS977 4.2 50.1 1.0
N D:THR968 4.2 60.4 1.0
N D:CYS977 4.2 45.7 1.0
CA D:CYS974 4.3 49.8 1.0
CA D:CYS890 4.3 65.1 1.0
C D:CYS967 4.3 57.4 1.0
CG2 D:THR892 4.5 63.4 1.0
O D:CYS974 4.6 41.9 1.0
CZ D:ARG962 4.6 45.2 1.0
N D:SER969 4.7 53.9 1.0
C D:CYS974 4.8 49.0 1.0
N D:CYS967 4.9 61.7 1.0
NH2 D:ARG962 4.9 64.6 1.0

Zinc binding site 2 out of 2 in 5vi8

Go back to Zinc Binding Sites List in 5vi8
Zinc binding site 2 out of 2 in the Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of A Mycobacterium Smegmatis Transcription Initiation Complex with An Upstream-Fork Promoter Fragment within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2002

b:0.8
occ:1.00
SG D:CYS62 2.1 82.8 1.0
SG D:CYS60 2.2 91.5 1.0
SG D:CYS75 2.2 67.8 1.0
SG D:CYS78 2.2 93.1 1.0
CB D:CYS75 2.9 81.6 1.0
CB D:CYS60 3.2 93.6 1.0
N D:CYS62 3.6 87.6 1.0
CB D:CYS62 3.6 0.8 1.0
CB D:CYS78 3.6 82.0 1.0
N D:GLY63 3.8 96.3 1.0
N D:CYS78 3.9 80.7 1.0
CA D:CYS62 4.0 98.1 1.0
N D:LYS64 4.1 92.9 1.0
N D:TYR61 4.3 84.1 1.0
C D:CYS62 4.3 90.4 1.0
C D:CYS60 4.4 85.8 1.0
CA D:CYS78 4.4 77.2 1.0
CA D:CYS75 4.4 66.0 1.0
CA D:CYS60 4.4 87.3 1.0
CB D:LYS64 4.6 96.2 1.0
C D:TYR61 4.7 85.5 1.0
CB D:ARG77 4.7 96.7 1.0
CA D:GLY63 4.8 97.8 1.0
C D:GLY63 4.9 91.2 1.0
O D:CYS60 4.9 89.8 1.0
CA D:LYS64 4.9 92.2 1.0
C D:CYS75 5.0 80.5 1.0
CA D:TYR61 5.0 91.1 1.0

Reference:

E.A.Hubin, M.Lilic, S.A.Darst, E.A.Campbell. Structural Insights Into the Mycobacteria Transcription Initiation Complex From Analysis of X-Ray Crystal Structures. Nat Commun V. 8 16072 2017.
ISSN: ESSN 2041-1723
PubMed: 28703128
DOI: 10.1038/NCOMMS16072
Page generated: Wed Dec 16 11:09:37 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy