Zinc in PDB 5vi5: Structure of Mycobacterium Smegmatis Transcription Initiation Complex with A Full Transcription Bubble

All present enzymatic activity of Structure of Mycobacterium Smegmatis Transcription Initiation Complex with A Full Transcription Bubble:
2.7.7.6;

The structure of Structure of Mycobacterium Smegmatis Transcription Initiation Complex with A Full Transcription Bubble, PDB code: 5vi5 was solved by S.A.Darst, E.A.Campbell, M.Lilic, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.02 / 3.20
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	132.062, 163.555, 139.964, 90.00, 107.90, 90.00
R / Rfree (%)	25.3 / 28

The binding sites of Zinc atom in the Structure of Mycobacterium Smegmatis Transcription Initiation Complex with A Full Transcription Bubble (pdb code 5vi5). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of Mycobacterium Smegmatis Transcription Initiation Complex with A Full Transcription Bubble, PDB code: 5vi5:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of Mycobacterium Smegmatis Transcription Initiation Complex with A Full Transcription Bubble


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Mycobacterium Smegmatis Transcription Initiation Complex with A Full Transcription Bubble within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2001 b:0.1 occ:1.00 SG D:CYS977 2.2 74.0 1.0 SG D:CYS974 2.3 57.9 1.0 SG D:CYS967 2.3 77.5 1.0 SG D:CYS890 2.3 0.2 1.0 CB D:CYS967 2.9 71.5 1.0 CB D:CYS977 3.0 59.0 1.0 CB D:CYS890 3.0 85.0 1.0 CA D:CYS967 3.4 79.5 1.0 CB D:CYS974 3.6 76.7 1.0 OG D:SER969 3.8 80.6 1.0 N D:CYS890 4.0 81.3 1.0 N D:THR968 4.1 86.4 1.0 CA D:CYS890 4.1 81.3 1.0 C D:CYS967 4.2 83.7 1.0 CA D:CYS977 4.2 64.0 1.0 N D:CYS977 4.3 71.9 1.0 N D:CYS974 4.4 47.4 1.0 CA D:CYS974 4.5 55.6 1.0 N D:CYS967 4.6 73.8 1.0 NH2 D:ARG962 4.6 61.2 1.0 O D:CYS974 4.8 52.2 1.0 NH1 D:ARG962 4.8 69.2 1.0 CG2 D:THR892 4.8 72.7 1.0 CZ D:ARG962 4.8 59.5 1.0 C D:CYS890 5.0 80.7 1.0 CB D:SER969 5.0 75.5 1.0 C D:CYS974 5.0 55.1 1.0

Zinc binding site 2 out of 2 in the Structure of Mycobacterium Smegmatis Transcription Initiation Complex with A Full Transcription Bubble


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Mycobacterium Smegmatis Transcription Initiation Complex with A Full Transcription Bubble within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2002 b:0.7 occ:1.00 SG D:CYS78 2.3 0.7 1.0 SG D:CYS62 2.3 90.5 1.0 SG D:CYS75 2.3 75.3 1.0 SG D:CYS60 2.7 0.1 1.0 CB D:CYS60 2.9 86.1 1.0 CB D:CYS75 3.0 69.3 1.0 CB D:CYS78 3.1 84.9 1.0 N D:CYS62 3.2 92.7 1.0 CB D:CYS62 3.4 90.4 1.0 N D:TYR61 3.8 0.7 1.0 CA D:CYS62 3.8 0.5 1.0 N D:CYS78 3.9 93.8 1.0 C D:CYS60 3.9 98.0 1.0 N D:GLY63 4.0 0.8 1.0 CA D:CYS60 4.0 91.5 1.0 CA D:CYS78 4.1 91.8 1.0 C D:CYS62 4.3 0.6 1.0 N D:LYS64 4.3 93.4 1.0 C D:TYR61 4.3 94.0 1.0 CA D:TYR61 4.5 87.3 1.0 CA D:CYS75 4.5 81.2 1.0 CB D:LYS64 4.5 94.4 1.0 O D:CYS60 4.6 88.4 1.0 CG D:LYS64 4.6 97.9 1.0 C D:CYS78 4.9 77.7 1.0 CB D:TYR61 5.0 72.0 1.0 CB D:ARG77 5.0 97.3 1.0

E.A.Hubin, M.Lilic, S.A.Darst, E.A.Campbell. Structural Insights Into the Mycobacteria Transcription Initiation Complex From Analysis of X-Ray Crystal Structures. Nat Commun V. 8 16072 2017.
ISSN: ESSN 2041-1723
PubMed: 28703128
DOI: 10.1038/NCOMMS16072