Zinc in PDB 5tl7: Crystal Structure of Sars-Cov Papain-Like Protease in Complex with C- Terminal Domain Mouse ISG15

All present enzymatic activity of Crystal Structure of Sars-Cov Papain-Like Protease in Complex with C- Terminal Domain Mouse ISG15:
2.7.7.48; 3.4.19.12; 3.4.22.69; 3.6.4.12; 3.6.4.13;

The structure of Crystal Structure of Sars-Cov Papain-Like Protease in Complex with C- Terminal Domain Mouse ISG15, PDB code: 5tl7 was solved by C.D.Daczkowski, J.V.Dzimianski, S.D.Pegan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	72.23 / 2.44
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	75.981, 98.191, 106.636, 90.00, 90.00, 90.00
R / Rfree (%)	19.2 / 26.7

The binding sites of Zinc atom in the Crystal Structure of Sars-Cov Papain-Like Protease in Complex with C- Terminal Domain Mouse ISG15 (pdb code 5tl7). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Sars-Cov Papain-Like Protease in Complex with C- Terminal Domain Mouse ISG15, PDB code: 5tl7:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Sars-Cov Papain-Like Protease in Complex with C- Terminal Domain Mouse ISG15


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Sars-Cov Papain-Like Protease in Complex with C- Terminal Domain Mouse ISG15 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn401 b:81.4 occ:1.00 SG B:CYS227 2.3 0.3 1.0 SG B:CYS190 2.3 69.9 1.0 SG B:CYS193 2.3 85.3 1.0 SG B:CYS225 2.3 94.5 1.0 CB B:CYS193 3.1 77.8 1.0 CB B:CYS227 3.2 0.3 1.0 CB B:CYS225 3.3 91.9 1.0 CB B:CYS190 3.3 71.9 1.0 N B:CYS193 3.7 72.5 1.0 N B:CYS227 3.9 0.3 1.0 CA B:CYS193 4.0 74.9 1.0 CA B:CYS227 4.1 0.5 1.0 CB B:HIS192 4.4 73.6 1.0 CG2 B:VAL226 4.5 0.8 1.0 ND1 B:HIS192 4.5 74.3 1.0 CA B:CYS225 4.6 92.6 1.0 C B:CYS225 4.7 96.1 1.0 C B:HIS192 4.7 72.7 1.0 CA B:CYS190 4.7 71.9 1.0 N B:VAL226 4.8 97.4 1.0 CA B:HIS192 4.9 73.8 1.0 CG B:HIS192 5.0 73.9 1.0 N B:HIS192 5.0 75.3 1.0 C B:CYS227 5.0 0.8 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Sars-Cov Papain-Like Protease in Complex with C- Terminal Domain Mouse ISG15


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Sars-Cov Papain-Like Protease in Complex with C- Terminal Domain Mouse ISG15 within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn401 b:74.1 occ:1.00 SG D:CYS190 2.1 64.5 1.0 SG D:CYS227 2.3 79.8 1.0 SG D:CYS225 2.4 86.9 1.0 SG D:CYS193 2.7 72.8 1.0 CB D:CYS190 3.2 66.9 1.0 CB D:CYS225 3.4 79.5 1.0 CB D:CYS227 3.4 89.1 1.0 CB D:CYS193 3.5 73.3 1.0 N D:CYS193 3.8 76.4 1.0 CB D:HIS192 4.2 78.0 1.0 ND1 D:HIS192 4.2 85.6 1.0 CA D:CYS193 4.3 76.0 1.0 N D:CYS227 4.4 83.2 1.0 NE D:ARG229 4.4 85.0 1.0 CA D:CYS227 4.5 86.1 1.0 CA D:CYS190 4.6 66.9 1.0 CG D:HIS192 4.7 84.5 1.0 CB D:ARG229 4.7 78.1 1.0 C D:HIS192 4.7 77.1 1.0 CA D:CYS225 4.8 80.5 1.0 CA D:HIS192 4.9 76.5 1.0 CD D:ARG229 4.9 81.2 1.0 N D:HIS192 5.0 73.0 1.0

C.M.Daczkowski, J.V.Dzimianski, J.R.Clasman, O.Goodwin, A.D.Mesecar, S.D.Pegan. Structural Insights Into the Interaction of Coronavirus Papain-Like Proteases and Interferon-Stimulated Gene Product 15 From Different Species. J. Mol. Biol. V. 429 1661 2017.
ISSN: ESSN 1089-8638
PubMed: 28438633
DOI: 10.1016/J.JMB.2017.04.011