Zinc in PDB 5ow3: Crystal Structure of A C-Terminally Truncated Trimeric Ectodomain of the Arabidopsis Thaliana Gamete Fusion Protein HAP2

The structure of Crystal Structure of A C-Terminally Truncated Trimeric Ectodomain of the Arabidopsis Thaliana Gamete Fusion Protein HAP2, PDB code: 5ow3 was solved by J.Fedry, P.Legrand, F.A.Rey, T.Krey, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.43 / 2.75
Space group	P 63
Cell size a, b, c (Å), α, β, γ (°)	77.255, 77.255, 219.526, 90.00, 90.00, 120.00
R / Rfree (%)	23.1 / 27.7

The binding sites of Zinc atom in the Crystal Structure of A C-Terminally Truncated Trimeric Ectodomain of the Arabidopsis Thaliana Gamete Fusion Protein HAP2 (pdb code 5ow3). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of A C-Terminally Truncated Trimeric Ectodomain of the Arabidopsis Thaliana Gamete Fusion Protein HAP2, PDB code: 5ow3:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of A C-Terminally Truncated Trimeric Ectodomain of the Arabidopsis Thaliana Gamete Fusion Protein HAP2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A C-Terminally Truncated Trimeric Ectodomain of the Arabidopsis Thaliana Gamete Fusion Protein HAP2 within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn1003 b:65.9 occ:1.00 OE1 C:GLU263 1.9 87.2 1.0 NE2 C:HIS361 2.3 64.0 1.0 NE2 C:HIS194 2.6 61.6 1.0 CE1 C:HIS194 2.8 61.2 1.0 CD C:GLU263 2.9 99.8 1.0 CE1 C:HIS361 3.1 63.7 1.0 CD2 C:HIS361 3.4 64.2 1.0 CD2 C:HIS194 3.5 61.8 1.0 CG C:GLU263 3.5 86.6 1.0 CB C:GLU263 3.7 78.0 1.0 ND1 C:HIS194 3.7 61.7 1.0 OE2 C:GLU263 3.9 92.3 1.0 N C:GLU263 4.0 55.5 1.0 CG C:HIS194 4.0 59.8 1.0 ND1 C:HIS361 4.3 64.1 1.0 CG C:HIS361 4.4 62.2 1.0 CA C:GLU263 4.5 76.8 1.0 O C:PRO261 4.9 57.0 1.0 C C:ARG262 5.0 59.3 1.0 CA C:ARG262 5.0 55.1 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of A C-Terminally Truncated Trimeric Ectodomain of the Arabidopsis Thaliana Gamete Fusion Protein HAP2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of A C-Terminally Truncated Trimeric Ectodomain of the Arabidopsis Thaliana Gamete Fusion Protein HAP2 within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn1004 b:81.3 occ:1.00 CD2 C:HIS321 2.5 43.9 1.0 CG C:HIS321 3.4 42.1 1.0 NE2 C:HIS321 3.5 43.7 1.0 CB C:HIS321 3.8 38.6 1.0 ND2 C:ASN326 3.9 40.5 1.0 CA C:HIS321 4.2 37.6 1.0 CE1 C:HIS321 4.6 43.4 1.0 ND1 C:HIS321 4.6 43.9 1.0 N C:ASN322 4.7 35.3 1.0 CG C:ASN326 5.0 51.9 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of A C-Terminally Truncated Trimeric Ectodomain of the Arabidopsis Thaliana Gamete Fusion Protein HAP2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of A C-Terminally Truncated Trimeric Ectodomain of the Arabidopsis Thaliana Gamete Fusion Protein HAP2 within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn1005 b:0.5 occ:1.00 OD2 C:ASP170 2.0 0.5 1.0 OD1 C:ASP173 2.7 97.9 1.0 OD2 C:ASP173 2.9 0.2 1.0 CG C:ASP173 2.9 97.1 1.0 CG C:ASP170 3.1 99.5 1.0 OD1 C:ASP170 3.5 0.3 1.0 CB C:ASP173 4.0 86.9 1.0 CB C:ASP170 4.3 88.9 1.0

J.Fedry, J.Forcina, P.Legrand, G.Pehau-Arnaudet, A.Haouz, M.Johnson, F.A.Rey, T.Krey. Evolutionary Diversification of the HAP2 Membrane Insertion Motifs to Drive Gamete Fusion Across Eukaryotes. Plos Biol. V. 16 06357 2018.
ISSN: ESSN 1545-7885
PubMed: 30102690
DOI: 10.1371/JOURNAL.PBIO.2006357