Zinc in PDB 5ohp: Crystal Structure of USP30 (C77A) in Complex with LYS6-Linked Diubiquitin

All present enzymatic activity of Crystal Structure of USP30 (C77A) in Complex with LYS6-Linked Diubiquitin:
3.4.19.12;

The structure of Crystal Structure of USP30 (C77A) in Complex with LYS6-Linked Diubiquitin, PDB code: 5ohp was solved by M.Gersch, D.Komander, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	82.08 / 2.80
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	117.504, 117.504, 138.842, 90.00, 90.00, 120.00
R / Rfree (%)	21.6 / 24.9

The binding sites of Zinc atom in the Crystal Structure of USP30 (C77A) in Complex with LYS6-Linked Diubiquitin (pdb code 5ohp). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of USP30 (C77A) in Complex with LYS6-Linked Diubiquitin, PDB code: 5ohp:

Zinc binding site 1 out of 1 in the Crystal Structure of USP30 (C77A) in Complex with LYS6-Linked Diubiquitin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of USP30 (C77A) in Complex with LYS6-Linked Diubiquitin within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn800 b:65.1 occ:1.00 SG A:CYS284 2.3 64.0 1.0 SG A:CYS237 2.3 62.9 1.0 SG A:CYS287 2.4 70.2 1.0 SG A:CYS234 2.4 65.4 1.0 CB A:CYS237 3.0 64.9 1.0 CB A:CYS234 3.0 58.2 1.0 CB A:CYS284 3.4 64.1 1.0 CB A:CYS287 3.5 73.6 1.0 N A:CYS237 3.8 64.9 1.0 N A:CYS287 3.9 71.7 1.0 CA A:CYS237 3.9 63.8 1.0 CA A:CYS287 4.2 73.7 1.0 CG2 A:THR306 4.2 63.3 1.0 OG1 A:THR306 4.3 56.2 1.0 CA A:CYS234 4.5 59.4 1.0 CB A:ASN286 4.6 67.6 1.0 C A:HIS236 4.7 65.2 1.0 C A:CYS237 4.7 62.6 1.0 CA A:CYS284 4.8 68.5 1.0 C A:ASN286 4.8 75.5 1.0 CB A:HIS236 4.9 64.4 1.0 CB A:THR306 4.9 62.8 1.0 CZ A:PHE308 5.0 59.3 1.0 C A:CYS234 5.0 62.3 1.0

M.Gersch, C.Gladkova, A.F.Schubert, M.A.Michel, S.Maslen, D.Komander. Mechanism and Regulation of the LYS6-Selective Deubiquitinase USP30. Nat. Struct. Mol. Biol. V. 24 920 2017.
ISSN: ESSN 1545-9985
PubMed: 28945249
DOI: 10.1038/NSMB.3475