Zinc in PDB 5ohn: Crystal Structure of USP30 in Covalent Complex with Ubiquitin Propargylamide (Low Resolution)

All present enzymatic activity of Crystal Structure of USP30 in Covalent Complex with Ubiquitin Propargylamide (Low Resolution):
3.4.19.12;

The structure of Crystal Structure of USP30 in Covalent Complex with Ubiquitin Propargylamide (Low Resolution), PDB code: 5ohn was solved by M.Gersch, D.Komander, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	157.47 / 3.60
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	181.834, 181.834, 94.957, 90.00, 90.00, 120.00
R / Rfree (%)	21.1 / 25.3

The binding sites of Zinc atom in the Crystal Structure of USP30 in Covalent Complex with Ubiquitin Propargylamide (Low Resolution) (pdb code 5ohn). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of USP30 in Covalent Complex with Ubiquitin Propargylamide (Low Resolution), PDB code: 5ohn:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of USP30 in Covalent Complex with Ubiquitin Propargylamide (Low Resolution)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of USP30 in Covalent Complex with Ubiquitin Propargylamide (Low Resolution) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn800 b:0.2 occ:1.00 SG A:CYS237 2.3 0.8 1.0 SG A:CYS287 2.3 0.3 1.0 SG A:CYS234 2.4 0.5 1.0 SG A:CYS284 2.4 0.8 1.0 CB A:CYS237 3.0 0.2 1.0 CB A:CYS234 3.4 0.5 1.0 CB A:CYS287 3.4 0.1 1.0 N A:CYS237 3.5 0.7 1.0 CB A:CYS284 3.6 0.5 1.0 N A:CYS287 3.8 0.1 1.0 CA A:CYS237 3.8 0.7 1.0 CA A:CYS287 4.2 0.6 1.0 CB A:HIS236 4.4 0.2 1.0 CB A:ASN286 4.5 0.8 1.0 C A:HIS236 4.6 0.7 1.0 C A:CYS237 4.6 0.0 1.0 N A:GLU238 4.7 0.2 1.0 C A:ASN286 4.8 0.1 1.0 CA A:CYS234 4.8 0.7 1.0 CG2 A:THR306 4.9 0.1 1.0 CA A:HIS236 5.0 0.5 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of USP30 in Covalent Complex with Ubiquitin Propargylamide (Low Resolution)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of USP30 in Covalent Complex with Ubiquitin Propargylamide (Low Resolution) within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn800 b:0.4 occ:1.00 SG C:CYS287 2.3 0.5 1.0 SG C:CYS237 2.3 0.3 1.0 SG C:CYS234 2.4 0.3 1.0 SG C:CYS284 2.4 0.0 1.0 CB C:CYS237 3.0 0.4 1.0 CB C:CYS234 3.4 1.0 1.0 CB C:CYS287 3.4 1.0 1.0 N C:CYS237 3.5 0.0 1.0 CB C:CYS284 3.6 1.0 1.0 N C:CYS287 3.8 0.3 1.0 CA C:CYS237 3.8 0.1 1.0 CA C:CYS287 4.2 0.0 1.0 CB C:ASN286 4.2 0.6 1.0 CB C:HIS236 4.4 0.1 1.0 C C:HIS236 4.6 0.5 1.0 C C:CYS237 4.7 0.4 1.0 N C:GLU238 4.7 0.4 1.0 C C:ASN286 4.8 0.6 1.0 CA C:CYS234 4.8 0.8 1.0 CG2 C:THR306 4.9 0.3 1.0 ND2 C:ASN286 4.9 0.7 1.0 CA C:HIS236 5.0 0.3 1.0 CA C:ASN286 5.0 0.3 1.0

M.Gersch, C.Gladkova, A.F.Schubert, M.A.Michel, S.Maslen, D.Komander. Mechanism and Regulation of the LYS6-Selective Deubiquitinase USP30. Nat. Struct. Mol. Biol. V. 24 920 2017.
ISSN: ESSN 1545-9985
PubMed: 28945249
DOI: 10.1038/NSMB.3475