Zinc in PDB 5obz: Low Resolution Structure of the P34CT/P44CT Minimal Complex

The structure of Low Resolution Structure of the P34CT/P44CT Minimal Complex, PDB code: 5obz was solved by E.Schoenwetter, W.Koelmel, D.R.Schmitt, J.Kuper, C.Kisker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.20 / 3.70
Space group	P 63 2 2
Cell size a, b, c (Å), α, β, γ (°)	138.212, 138.212, 95.235, 90.00, 90.00, 120.00
R / Rfree (%)	19.6 / 24.6

The binding sites of Zinc atom in the Low Resolution Structure of the P34CT/P44CT Minimal Complex (pdb code 5obz). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Low Resolution Structure of the P34CT/P44CT Minimal Complex, PDB code: 5obz:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Low Resolution Structure of the P34CT/P44CT Minimal Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Low Resolution Structure of the P34CT/P44CT Minimal Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn601 b:0.9 occ:1.00 SG B:CYS404 2.3 0.3 1.0 SG B:CYS487 2.3 0.0 1.0 SG B:CYS484 2.3 0.3 1.0 SG B:CYS401 2.4 0.8 1.0 CB B:CYS404 3.3 0.7 1.0 CB B:CYS401 3.3 0.5 1.0 CB B:CYS487 3.3 0.7 1.0 CB B:CYS484 3.5 0.7 1.0 N B:CYS484 3.9 0.9 1.0 N B:CYS404 4.0 0.2 1.0 N B:CYS487 4.1 0.6 1.0 CA B:CYS484 4.1 0.4 1.0 CA B:CYS404 4.2 0.9 1.0 CA B:CYS487 4.3 0.4 1.0 CD A:LYS155 4.3 0.2 1.0 C B:CYS484 4.6 0.6 1.0 CB B:ALA406 4.7 0.2 1.0 O B:CYS484 4.7 0.0 1.0 CA B:CYS401 4.8 0.2 1.0 CG A:LYS155 4.9 0.8 1.0 C B:CYS404 4.9 0.2 1.0 CB B:ALA403 5.0 0.3 1.0 CB A:LYS155 5.0 0.8 1.0

Zinc binding site 2 out of 2 in the Low Resolution Structure of the P34CT/P44CT Minimal Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Low Resolution Structure of the P34CT/P44CT Minimal Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn602 b:0.8 occ:1.00 SG B:CYS479 2.3 0.0 1.0 SG B:CYS498 2.3 0.1 1.0 SG B:CYS476 2.3 0.2 1.0 SG B:CYS501 2.4 0.0 1.0 CB B:CYS479 3.2 0.4 1.0 N B:CYS501 3.3 0.5 1.0 CB B:CYS476 3.3 0.6 1.0 CB B:CYS498 3.3 0.3 1.0 CB B:CYS501 3.5 0.4 1.0 N B:CYS479 3.6 0.3 1.0 CA B:CYS501 3.9 0.2 1.0 C B:GLY500 4.0 0.3 1.0 CA B:CYS479 4.0 0.7 1.0 CA B:GLY500 4.1 0.9 1.0 CB B:THR478 4.4 0.8 1.0 N B:GLY500 4.4 0.0 1.0 CA B:CYS498 4.7 0.8 1.0 CA B:CYS476 4.7 0.1 1.0 C B:THR478 4.7 0.1 1.0 C B:CYS479 4.9 0.2 1.0 O B:GLY500 4.9 0.4 1.0 CA B:THR478 4.9 0.6 1.0 C B:CYS498 5.0 0.7 1.0 N B:GLY480 5.0 0.1 1.0

L.Radu, E.Schoenwetter, C.Braun, J.Marcoux, W.Koelmel, D.R.Schmitt, J.Kuper, S.Cianferani, J.M.Egly, A.Poterszman, C.Kisker. The Intricate Network Between the P34 and P44 Subunits Is Central to the Activity of the Transcription/Dna Repair Factor Tfiih. Nucleic Acids Res. V. 45 10872 2017.
ISSN: ESSN 1362-4962
PubMed: 28977422
DOI: 10.1093/NAR/GKX743