Zinc in PDB 5nus: Structure of A Minimal Complex Between P44 and P34 From Chaetomium Thermophilum

The structure of Structure of A Minimal Complex Between P44 and P34 From Chaetomium Thermophilum, PDB code: 5nus was solved by W.Koelmel, E.Schoenwetter, J.Kuper, D.R.Schmitt, C.Kisker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.42 / 2.20
Space group	P 63 2 2
Cell size a, b, c (Å), α, β, γ (°)	147.056, 147.056, 87.599, 90.00, 90.00, 120.00
R / Rfree (%)	21 / 22.7

The binding sites of Zinc atom in the Structure of A Minimal Complex Between P44 and P34 From Chaetomium Thermophilum (pdb code 5nus). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of A Minimal Complex Between P44 and P34 From Chaetomium Thermophilum, PDB code: 5nus:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of A Minimal Complex Between P44 and P34 From Chaetomium Thermophilum


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of A Minimal Complex Between P44 and P34 From Chaetomium Thermophilum within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn601 b:62.5 occ:1.00 SG B:CYS487 2.3 64.1 1.0 SG B:CYS404 2.3 75.4 1.0 SG B:CYS401 2.3 71.0 1.0 SG B:CYS484 2.6 66.6 1.0 H B:CYS404 2.9 69.0 1.0 HB3 B:CYS404 2.9 62.9 1.0 HB3 B:CYS401 3.0 74.9 1.0 CB B:CYS401 3.1 62.4 1.0 HB2 B:CYS487 3.1 72.3 1.0 H B:CYS484 3.1 80.0 1.0 HB2 B:CYS401 3.2 74.9 1.0 CB B:CYS404 3.2 52.4 1.0 HB3 B:CYS484 3.3 78.3 1.0 CB B:CYS487 3.3 60.3 1.0 CB B:CYS484 3.5 65.2 1.0 HB3 B:ALA403 3.6 65.3 1.0 N B:CYS404 3.6 57.5 1.0 HD3 A:LYS155 3.7 76.2 1.0 H B:CYS487 3.8 74.1 1.0 N B:CYS484 3.9 66.7 1.0 HB2 B:CYS404 4.0 62.9 1.0 CA B:CYS404 4.0 56.7 1.0 HB3 B:CYS487 4.0 72.3 1.0 HB3 B:ALA406 4.1 79.6 1.0 HZ1 A:LYS155 4.1 85.1 1.0 HE2 A:LYS155 4.2 81.2 1.0 H B:ALA406 4.3 80.8 1.0 CA B:CYS484 4.3 68.4 1.0 HB2 B:CYS484 4.3 78.3 1.0 N B:CYS487 4.3 61.7 1.0 H B:ALA403 4.4 73.9 1.0 CA B:CYS487 4.4 60.4 1.0 HB3 B:PHE483 4.5 80.3 1.0 CD A:LYS155 4.5 63.5 1.0 CB B:ALA403 4.5 54.4 1.0 HG2 A:LYS155 4.5 72.7 1.0 HB2 A:LYS155 4.6 68.9 1.0 CA B:CYS401 4.6 67.0 1.0 HB2 B:ASP486 4.6 81.9 1.0 H B:LEU405 4.7 77.4 1.0 CE A:LYS155 4.7 67.6 1.0 HA B:PHE483 4.7 81.5 1.0 C B:ALA403 4.7 56.2 1.0 C B:CYS404 4.7 60.7 1.0 HA B:CYS487 4.7 72.5 1.0 HA B:CYS404 4.7 68.1 1.0 HB2 B:ALA406 4.7 79.6 1.0 HD1 B:PHE483 4.8 74.5 1.0 O A:HOH307 4.8 56.5 1.0 NZ A:LYS155 4.8 70.9 1.0 CB B:ALA406 4.9 66.3 1.0 HB1 B:ALA403 4.9 65.3 1.0 C B:CYS484 4.9 70.6 1.0 N B:LEU405 4.9 64.5 1.0 HA B:CYS401 4.9 80.4 1.0 CG A:LYS155 5.0 60.6 1.0 N B:ALA403 5.0 61.6 1.0 CA B:ALA403 5.0 57.1 1.0

Zinc binding site 2 out of 2 in the Structure of A Minimal Complex Between P44 and P34 From Chaetomium Thermophilum


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of A Minimal Complex Between P44 and P34 From Chaetomium Thermophilum within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn602 b:67.9 occ:1.00 SG B:CYS501 2.2 64.7 1.0 SG B:CYS479 2.2 81.9 1.0 SG B:CYS498 2.2 76.8 1.0 SG B:CYS476 2.4 80.3 1.0 H B:CYS501 2.8 80.4 1.0 HB3 B:CYS476 2.9 93.9 1.0 H B:CYS479 3.0 0.2 1.0 HB3 B:CYS479 3.0 90.9 1.0 CB B:CYS476 3.1 78.3 1.0 HB3 B:CYS498 3.2 80.8 1.0 CB B:CYS479 3.2 75.7 1.0 HB2 B:CYS476 3.2 93.9 1.0 CB B:CYS498 3.3 67.3 1.0 N B:CYS501 3.3 67.0 1.0 CB B:CYS501 3.4 69.1 1.0 HB2 B:CYS498 3.5 80.8 1.0 HB B:THR478 3.5 0.8 1.0 HB2 B:CYS501 3.6 82.9 1.0 N B:CYS479 3.7 86.8 1.0 HA3 B:GLY500 3.8 79.0 1.0 CA B:CYS501 3.9 67.4 1.0 HB2 B:CYS479 4.0 90.9 1.0 HA B:CYS501 4.0 80.8 1.0 CA B:CYS479 4.1 84.7 1.0 C B:GLY500 4.1 67.5 1.0 H B:GLY500 4.2 77.6 1.0 HB3 B:CYS501 4.3 82.9 1.0 CA B:GLY500 4.3 65.8 1.0 O B:HOH703 4.4 66.5 1.0 HE2 B:PHE483 4.4 80.6 1.0 HE1 B:TRP385 4.4 85.3 1.0 HZ B:PHE483 4.5 77.2 1.0 CB B:THR478 4.5 93.2 1.0 CA B:CYS476 4.6 81.7 1.0 H B:GLY480 4.6 0.3 1.0 N B:GLY500 4.6 64.7 1.0 H B:THR478 4.6 0.9 1.0 CA B:CYS498 4.6 65.3 1.0 HB2 B:LYS481 4.7 89.3 1.0 H B:LYS481 4.7 94.0 1.0 HA B:CYS479 4.8 0.7 1.0 C B:THR478 4.8 92.5 1.0 HZ2 B:TRP385 4.8 82.3 1.0 C B:CYS498 4.9 64.8 1.0 NE1 B:TRP385 4.9 71.1 1.0 C B:CYS479 4.9 85.1 1.0 C B:CYS476 5.0 87.0 1.0 HA B:CYS498 5.0 78.3 1.0

L.Radu, E.Schoenwetter, C.Braun, J.Marcoux, W.Koelmel, D.R.Schmitt, J.Kuper, S.Cianferani, J.M.Egly, A.Poterszman, C.Kisker. The Intricate Network Between the P34 and P44 Subunits Is Central to the Activity of the Transcription/Dna Repair Factor Tfiih. Nucleic Acids Res. V. 45 10872 2017.
ISSN: ESSN 1362-4962
PubMed: 28977422
DOI: 10.1093/NAR/GKX743