Zinc in PDB 5lb3: Crystal Structure of Human RECQL5 Helicase in Complex with Adp/Mg.

All present enzymatic activity of Crystal Structure of Human RECQL5 Helicase in Complex with Adp/Mg.:
3.6.4.12;

The structure of Crystal Structure of Human RECQL5 Helicase in Complex with Adp/Mg., PDB code: 5lb3 was solved by J.A.Newman, H.Aitkenhead, P.Savitsky, T.Krojer, F.Von Delft, C.H.Arrowsmith, A.M.Edwards, C.Bountra, O.Gileadi, Structural Genomicsconsortium (Sgc), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.88 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	72.757, 62.943, 104.703, 90.00, 95.44, 90.00
R / Rfree (%)	19.5 / 23.4

The structure of Crystal Structure of Human RECQL5 Helicase in Complex with Adp/Mg. also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Zinc atom in the Crystal Structure of Human RECQL5 Helicase in Complex with Adp/Mg. (pdb code 5lb3). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Human RECQL5 Helicase in Complex with Adp/Mg., PDB code: 5lb3:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Human RECQL5 Helicase in Complex with Adp/Mg.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Human RECQL5 Helicase in Complex with Adp/Mg. within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn501 b:24.0 occ:1.00 SG B:CYS434 2.3 25.9 1.0 SG B:CYS411 2.4 22.1 1.0 SG B:CYS427 2.4 25.3 1.0 SG B:CYS431 2.4 21.4 1.0 CB B:CYS427 3.1 20.3 1.0 CB B:CYS434 3.1 21.3 1.0 CB B:CYS411 3.2 21.0 1.0 CB B:CYS431 3.5 23.6 1.0 CA B:CYS411 3.6 21.0 1.0 N B:CYS431 4.0 23.1 1.0 N B:ARG412 4.1 21.4 1.0 N B:CYS434 4.2 20.6 1.0 C B:CYS411 4.3 22.2 1.0 CD2 B:HIS413 4.3 23.5 1.0 CA B:CYS434 4.3 24.2 1.0 CA B:CYS431 4.3 23.0 1.0 CA B:CYS427 4.6 32.9 1.0 C B:GLY430 4.6 23.6 1.0 N B:HIS413 4.7 19.7 1.0 O B:HOH906 4.7 37.5 1.0 N B:GLY430 4.7 27.4 1.0 O B:HOH804 4.8 24.3 1.0 N B:CYS411 4.9 25.9 1.0 C B:CYS431 4.9 22.4 1.0 CB B:HIS413 4.9 22.4 1.0 O B:CYS427 5.0 35.0 1.0 CG B:HIS413 5.0 21.0 1.0 O B:CYS431 5.0 26.0 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Human RECQL5 Helicase in Complex with Adp/Mg.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Human RECQL5 Helicase in Complex with Adp/Mg. within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn501 b:21.8 occ:1.00 SG E:CYS427 2.3 22.9 1.0 SG E:CYS431 2.3 20.0 1.0 SG E:CYS411 2.3 19.6 1.0 SG E:CYS434 2.4 20.9 1.0 CB E:CYS411 3.1 21.9 1.0 CB E:CYS434 3.2 17.9 1.0 CB E:CYS431 3.4 19.2 1.0 CB E:CYS427 3.5 29.4 1.0 CA E:CYS411 3.6 21.1 1.0 N E:CYS427 3.6 24.6 1.0 CA E:CYS427 4.0 25.0 1.0 N E:ARG412 4.0 17.7 1.0 N E:CYS431 4.1 17.9 1.0 N E:ALA428 4.1 23.9 1.0 C E:CYS411 4.2 21.9 1.0 N E:CYS434 4.2 16.5 1.0 CA E:CYS431 4.3 18.7 1.0 CA E:CYS434 4.3 18.2 1.0 C E:CYS427 4.4 24.0 1.0 CD2 E:HIS413 4.6 20.8 1.0 N E:HIS413 4.6 17.4 1.0 C E:GLY430 4.7 16.4 1.0 C E:ALA426 4.7 25.2 1.0 C E:CYS431 4.8 21.4 1.0 O E:CYS431 4.8 18.7 1.0 O E:HOH731 4.8 22.2 1.0 N E:CYS411 4.9 22.1 1.0 CB E:HIS413 4.9 19.6 1.0 N E:GLY430 5.0 22.8 1.0 CA E:ALA426 5.0 22.0 1.0

J.A.Newman, H.Aitkenhead, P.Savitsky, O.Gileadi. Insights Into the Recq Helicase Mechanism Revealed By the Structure of the Helicase Domain of Human RECQL5. Nucleic Acids Res. V. 45 4231 2017.
ISSN: ESSN 1362-4962
PubMed: 28100692
DOI: 10.1093/NAR/GKW1362