Zinc in PDB 5kvl: Humanized 10G4 Anti-Leukotriene C4 Antibody Fab Fragment in Complex with Leukotriene C4

The structure of Humanized 10G4 Anti-Leukotriene C4 Antibody Fab Fragment in Complex with Leukotriene C4, PDB code: 5kvl was solved by T.Huxford, A.King, J.K.Fleming, J.M.Wojciak, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	90.08 / 1.74
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	51.880, 41.631, 93.126, 90.00, 104.69, 90.00
R / Rfree (%)	19 / 22

The binding sites of Zinc atom in the Humanized 10G4 Anti-Leukotriene C4 Antibody Fab Fragment in Complex with Leukotriene C4 (pdb code 5kvl). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Humanized 10G4 Anti-Leukotriene C4 Antibody Fab Fragment in Complex with Leukotriene C4, PDB code: 5kvl:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Humanized 10G4 Anti-Leukotriene C4 Antibody Fab Fragment in Complex with Leukotriene C4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Humanized 10G4 Anti-Leukotriene C4 Antibody Fab Fragment in Complex with Leukotriene C4 within 5.0Å range: probe atom residue distance (Å) B Occ H:Zn301 b:21.9 occ:1.00 O L:HOH500 1.8 20.3 1.0 O L:HOH402 2.0 23.4 1.0 NE2 H:HIS162 2.0 17.7 1.0 O H:HOH566 2.1 29.1 1.0 O L:HOH535 2.1 39.6 1.0 O H:HOH417 2.3 35.0 1.0 CD2 H:HIS162 3.0 16.9 1.0 CE1 H:HIS162 3.1 17.2 1.0 OD1 L:ASN138 3.5 25.2 1.0 CG H:HIS162 4.1 16.2 1.0 ND1 H:HIS162 4.1 16.9 1.0 O H:GLY160 4.3 15.7 1.0 CG L:ASN138 4.4 22.0 1.0 ND2 L:ASN138 4.4 24.1 1.0 O L:HOH508 4.4 21.3 1.0 CA H:GLY160 4.5 16.4 1.0 OG1 H:THR181 4.5 16.1 1.0 OD1 L:ASN137 4.5 24.2 1.0 O H:HOH517 4.5 24.6 1.0 C H:GLY160 4.6 15.9 1.0 OG L:SER174 4.7 14.9 1.0

Zinc binding site 2 out of 3 in the Humanized 10G4 Anti-Leukotriene C4 Antibody Fab Fragment in Complex with Leukotriene C4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Humanized 10G4 Anti-Leukotriene C4 Antibody Fab Fragment in Complex with Leukotriene C4 within 5.0Å range: probe atom residue distance (Å) B Occ L:Zn301 b:31.9 occ:1.00 ND1 L:HIS189 2.0 28.0 1.0 OD2 L:ASP151 2.1 28.8 1.0 CE1 L:HIS189 2.9 28.1 1.0 CG L:ASP151 3.1 28.6 1.0 CG L:HIS189 3.1 27.6 1.0 CB L:ASP151 3.5 28.4 1.0 CB L:HIS189 3.6 27.5 1.0 CA L:HIS189 3.8 27.3 1.0 NE2 L:HIS189 4.1 28.1 1.0 CD2 L:HIS189 4.2 28.1 1.0 OD1 L:ASP151 4.3 29.0 1.0 O L:LYS188 4.3 28.0 1.0 N L:HIS189 4.6 27.6 1.0 C L:LYS188 4.7 27.8 1.0 N L:LYS190 4.9 26.6 1.0 CG L:LYS188 4.9 28.8 1.0 C L:HIS189 5.0 27.1 1.0 CA L:ASP151 5.0 28.4 1.0

Zinc binding site 3 out of 3 in the Humanized 10G4 Anti-Leukotriene C4 Antibody Fab Fragment in Complex with Leukotriene C4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Humanized 10G4 Anti-Leukotriene C4 Antibody Fab Fragment in Complex with Leukotriene C4 within 5.0Å range: probe atom residue distance (Å) B Occ L:Zn302 b:45.7 occ:1.00 NE2 L:HIS189 2.0 28.1 1.0 CE1 L:HIS189 3.0 28.1 1.0 CD2 L:HIS189 3.0 28.1 1.0 CG L:ASP185 4.0 26.2 1.0 O L:HOH404 4.0 48.4 1.0 CB L:ASP185 4.0 24.9 1.0 ND1 L:HIS189 4.1 28.0 1.0 CG L:HIS189 4.1 27.6 1.0 OD1 L:ASP185 4.2 28.1 1.0 OD2 L:ASP185 4.4 28.2 1.0 O L:ASP185 4.4 24.6 1.0 CA L:ASP185 4.6 24.6 1.0 CD L:LYS188 4.7 30.6 1.0 C L:ASP185 4.9 24.6 1.0

T.Huxford, A.King, J.K.Fleming, J.M.Wojciak. Humanized 10G4 Anti-Leukotriene C4 Antibody Fab Fragment in Complex with Leukotriene C4 To Be Published.