Zinc in PDB 5jch: Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned).

The structure of Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned)., PDB code: 5jch was solved by S.Cusack, E.Uchikawa, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.95
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	72.080, 139.730, 103.190, 90.00, 110.14, 90.00
R / Rfree (%)	25.6 / 27

The structure of Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned). also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Zinc atom in the Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned). (pdb code 5jch). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned)., PDB code: 5jch:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned).


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned). within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1001 b:74.3 occ:1.00 SG A:CYS939 2.3 75.6 1.0 SG A:CYS884 2.3 77.9 1.0 SG A:CYS936 2.3 77.2 1.0 SG A:CYS881 2.3 72.7 1.0 CB A:CYS936 3.3 77.7 1.0 CB A:CYS881 3.4 72.8 1.0 CB A:CYS939 3.4 77.7 1.0 CB A:CYS884 3.4 81.1 1.0 N A:CYS884 4.0 82.2 1.0 N A:CYS939 4.2 79.7 1.0 CA A:CYS884 4.2 83.6 1.0 CA A:CYS939 4.3 78.7 1.0 CB A:GLN941 4.5 75.1 1.0 CZ2 A:TRP943 4.6 74.1 1.0 NE1 A:TRP943 4.6 74.2 1.0 CA A:CYS936 4.8 78.2 1.0 CA A:CYS881 4.8 72.9 1.0 CB A:ASN883 4.8 75.5 1.0 C A:CYS884 4.9 87.8 1.0 C A:CYS939 5.0 78.2 1.0 CE2 A:TRP943 5.0 73.8 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned).


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned). within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1001 b:0.7 occ:1.00 SG B:CYS939 2.3 0.2 1.0 SG B:CYS884 2.3 0.2 1.0 SG B:CYS881 2.3 97.9 1.0 SG B:CYS936 2.3 0.6 1.0 CB B:CYS936 3.3 0.7 1.0 CB B:CYS881 3.3 97.4 1.0 CB B:CYS939 3.4 0.7 1.0 CB B:CYS884 3.4 0.7 1.0 N B:CYS884 4.0 0.6 1.0 N B:CYS939 4.2 0.0 1.0 CA B:CYS884 4.3 0.9 1.0 CA B:CYS939 4.4 0.5 1.0 CB B:GLN941 4.5 97.0 1.0 CZ2 B:TRP943 4.6 1.0 1.0 NE1 B:TRP943 4.6 0.6 1.0 CA B:CYS936 4.8 0.5 1.0 CA B:CYS881 4.8 96.8 1.0 CB B:ASN883 4.8 94.6 1.0 C B:CYS884 4.9 0.1 1.0 CE2 B:TRP943 5.0 0.6 1.0 C B:CYS939 5.0 0.3 1.0

E.Uchikawa, M.Lethier, H.Malet, J.Brunel, D.Gerlier, S.Cusack. Structural Analysis of Dsrna Binding to Anti-Viral Pattern Recognition Receptors LGP2 and MDA5. Mol.Cell V. 62 586 2016.
ISSN: ISSN 1097-2765
PubMed: 27203181
DOI: 10.1016/J.MOLCEL.2016.04.021