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Zinc in PDB 4yfx: Escherichia Coli Rna Polymerase in Complex with Myxopyronin B

Enzymatic activity of Escherichia Coli Rna Polymerase in Complex with Myxopyronin B

All present enzymatic activity of Escherichia Coli Rna Polymerase in Complex with Myxopyronin B:
2.7.7.6;

Protein crystallography data

The structure of Escherichia Coli Rna Polymerase in Complex with Myxopyronin B, PDB code: 4yfx was solved by V.Molodtsov, P.R.Fleming, C.J.Eyermann, A.D.Ferguson, M.A.Foulk, D.C.Mckinney, C.E.Masse, E.T.Buurman, K.S.Murakami, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 29.95 / 3.84
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 188.520, 205.177, 310.140, 90.00, 90.00, 90.00
R / Rfree (%) 23.6 / 30

Other elements in 4yfx:

The structure of Escherichia Coli Rna Polymerase in Complex with Myxopyronin B also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Escherichia Coli Rna Polymerase in Complex with Myxopyronin B (pdb code 4yfx). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Escherichia Coli Rna Polymerase in Complex with Myxopyronin B, PDB code: 4yfx:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in 4yfx

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Zinc binding site 1 out of 4 in the Escherichia Coli Rna Polymerase in Complex with Myxopyronin B


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Escherichia Coli Rna Polymerase in Complex with Myxopyronin B within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2002

b:0.1
occ:1.00
SG D:CYS85 2.1 1.0 1.0
SG D:CYS70 2.1 0.6 1.0
SG D:CYS88 2.2 0.2 1.0
SG D:CYS72 2.3 0.7 1.0
CB D:CYS85 2.3 0.1 1.0
CB D:CYS72 3.1 0.5 1.0
N D:CYS72 3.2 0.3 1.0
CB D:CYS70 3.2 0.6 1.0
CA D:CYS72 3.6 0.8 1.0
N D:GLY73 3.6 0.5 1.0
CA D:CYS85 3.7 0.5 1.0
N D:LEU71 3.9 0.8 1.0
CB D:CYS88 3.9 0.4 1.0
N D:CYS88 4.0 0.1 1.0
C D:CYS72 4.1 0.0 1.0
O D:CYS88 4.1 0.7 1.0
C D:LEU71 4.3 0.9 1.0
CA D:CYS70 4.3 0.6 1.0
N D:LYS74 4.3 0.3 1.0
C D:CYS70 4.3 0.7 1.0
CA D:CYS88 4.4 0.2 1.0
CD1 D:TYR75 4.5 0.7 1.0
C D:CYS85 4.5 0.1 1.0
CA D:LEU71 4.6 0.8 1.0
N D:CYS85 4.7 0.3 1.0
CA D:GLY73 4.7 0.9 1.0
CB D:LYS87 4.7 0.9 1.0
C D:CYS88 4.7 0.5 1.0
CE1 D:TYR75 4.8 0.4 1.0
CB D:LYS74 4.9 0.8 1.0
N D:LYS87 4.9 0.3 1.0
C D:GLY73 5.0 0.8 1.0

Zinc binding site 2 out of 4 in 4yfx

Go back to Zinc Binding Sites List in 4yfx
Zinc binding site 2 out of 4 in the Escherichia Coli Rna Polymerase in Complex with Myxopyronin B


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Escherichia Coli Rna Polymerase in Complex with Myxopyronin B within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2003

b:0.9
occ:1.00
SG D:CYS895 2.1 0.0 1.0
SG D:CYS888 2.1 0.4 1.0
SG D:CYS814 2.2 0.2 1.0
SG D:CYS898 2.2 0.2 1.0
CB D:CYS898 2.4 0.4 1.0
NH2 D:ARG883 2.5 0.6 1.0
CB D:CYS888 2.9 0.0 1.0
CB D:CYS895 2.9 0.7 1.0
CB D:CYS814 3.1 0.5 1.0
CA D:CYS888 3.3 0.9 1.0
CA D:CYS898 3.5 0.8 1.0
N D:CYS898 3.6 0.4 1.0
N D:ASP889 3.7 0.8 1.0
CZ D:ARG883 3.7 0.6 1.0
N D:CYS895 3.8 0.2 1.0
C D:CYS888 3.8 0.9 1.0
CA D:CYS895 3.9 1.0 1.0
N D:CYS814 3.9 0.6 1.0
CA D:CYS814 4.1 0.2 1.0
NE D:ARG883 4.2 0.9 1.0
N D:THR890 4.3 0.3 1.0
C D:CYS895 4.5 0.8 1.0
O D:CYS895 4.5 0.5 1.0
N D:CYS888 4.5 0.2 1.0
CG2 D:THR890 4.7 0.1 1.0
C D:CYS898 4.8 0.8 1.0
CB D:THR890 4.8 0.2 1.0
NH1 D:ARG883 4.8 0.6 1.0
O D:CYS888 4.8 0.1 1.0
C D:HIS897 4.8 0.8 1.0
CA D:ASP889 5.0 0.4 1.0
N D:GLY815 5.0 0.7 1.0
CG2 D:THR816 5.0 0.8 1.0
C D:CYS814 5.0 0.2 1.0

Zinc binding site 3 out of 4 in 4yfx

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Zinc binding site 3 out of 4 in the Escherichia Coli Rna Polymerase in Complex with Myxopyronin B


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Escherichia Coli Rna Polymerase in Complex with Myxopyronin B within 5.0Å range:
probe atom residue distance (Å) B Occ
J:Zn2002

b:0.7
occ:1.00
SG J:CYS88 2.1 0.8 1.0
SG J:CYS85 2.1 0.0 1.0
SG J:CYS70 2.1 0.5 1.0
SG J:CYS72 2.2 1.0 1.0
CB J:CYS72 2.7 0.9 1.0
CB J:CYS85 2.7 0.7 1.0
N J:CYS72 2.9 0.4 1.0
N J:CYS88 3.2 0.9 1.0
CA J:CYS72 3.3 0.1 1.0
CB J:CYS88 3.4 0.9 1.0
N J:GLY73 3.7 0.2 1.0
CB J:CYS70 3.7 0.5 1.0
O J:CYS88 3.7 0.7 1.0
CA J:CYS88 3.8 0.0 1.0
CB J:LYS87 3.9 0.4 1.0
N J:LEU71 3.9 0.9 1.0
C J:CYS72 4.0 0.8 1.0
C J:LEU71 4.0 0.1 1.0
CA J:CYS85 4.2 0.9 1.0
C J:CYS88 4.2 0.1 1.0
C J:LYS87 4.3 0.4 1.0
C J:CYS70 4.4 0.7 1.0
CA J:LEU71 4.5 0.6 1.0
CA J:LYS87 4.5 0.2 1.0
N J:LYS87 4.5 0.3 1.0
N J:LYS74 4.6 0.8 1.0
CA J:CYS70 4.6 0.0 1.0
C J:CYS85 4.7 0.3 1.0
CB J:LEU71 4.9 0.2 1.0
CA J:GLY73 4.9 0.2 1.0
CG J:LYS87 5.0 0.4 1.0

Zinc binding site 4 out of 4 in 4yfx

Go back to Zinc Binding Sites List in 4yfx
Zinc binding site 4 out of 4 in the Escherichia Coli Rna Polymerase in Complex with Myxopyronin B


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Escherichia Coli Rna Polymerase in Complex with Myxopyronin B within 5.0Å range:
probe atom residue distance (Å) B Occ
J:Zn2003

b:0.5
occ:1.00
SG J:CYS895 2.1 0.1 1.0
SG J:CYS898 2.1 0.7 1.0
SG J:CYS888 2.2 0.5 1.0
CB J:CYS898 2.3 0.8 1.0
CB J:CYS888 2.6 0.5 1.0
SG J:CYS814 2.9 0.1 1.0
CA J:CYS888 3.5 0.3 1.0
CB J:CYS895 3.5 0.6 1.0
CA J:CYS898 3.5 0.2 1.0
NH2 J:ARG883 3.6 0.5 1.0
N J:CYS898 3.6 1.0 1.0
CB J:CYS814 4.0 0.9 1.0
OG1 J:THR890 4.1 0.4 1.0
O J:CYS895 4.1 0.5 1.0
N J:CYS895 4.1 0.4 1.0
N J:ASP889 4.1 0.1 1.0
C J:CYS888 4.2 0.4 1.0
CA J:CYS895 4.2 0.1 1.0
C J:CYS895 4.5 0.1 1.0
N J:CYS888 4.6 0.6 1.0
CZ J:ARG883 4.6 0.3 1.0
CG2 J:THR816 4.6 0.9 1.0
N J:THR890 4.7 0.8 1.0
N J:CYS814 4.7 0.3 1.0
C J:CYS898 4.7 0.4 1.0
NE J:ARG883 4.9 0.2 1.0
CB J:THR890 4.9 0.9 1.0
OD1 J:ASP813 4.9 0.7 1.0
C J:HIS897 4.9 0.4 1.0

Reference:

V.Molodtsov, P.R.Fleming, C.J.Eyermann, A.D.Ferguson, M.A.Foulk, D.C.Mckinney, C.E.Masse, E.T.Buurman, K.S.Murakami. X-Ray Crystal Structures of Escherichia Coli Rna Polymerase with Switch Region Binding Inhibitors Enable Rational Design of Squaramides with An Improved Fraction Unbound to Human Plasma Protein To Be Published.
Page generated: Sun Oct 27 11:10:52 2024

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