Zinc in PDB 4w74: Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group

The structure of Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group, PDB code: 4w74 was solved by D.J.Leibly, G.S.Waldo, T.O.Yeates, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	88.27 / 2.10
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	67.440, 119.790, 130.570, 90.00, 89.99, 90.00
R / Rfree (%)	21.2 / 23.5

The binding sites of Zinc atom in the Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group (pdb code 4w74). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group, PDB code: 4w74:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6;

Zinc binding site 1 out of 6 in the Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn301 b:45.0 occ:1.00 NE2 A:HIS118 2.2 39.4 1.0 SG G:CYS115 2.6 62.6 1.0 O A:HOH402 2.7 52.0 1.0 CE1 A:HIS118 3.1 42.0 1.0 SG A:CYS115 3.2 66.7 1.0 CB A:CYS115 3.2 33.8 1.0 CD2 A:HIS118 3.3 39.5 1.0 CB G:CYS115 3.4 39.9 1.0 ND1 A:HIS118 4.2 43.7 1.0 CD1 A:LEU15 4.4 40.2 1.0 CG A:HIS118 4.4 40.7 1.0 CA G:CYS115 4.7 35.5 1.0 CA A:CYS115 4.7 39.6 1.0 O A:CYS115 4.9 44.8 1.0

Zinc binding site 2 out of 6 in the Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn301 b:42.6 occ:1.00 O B:HOH401 1.9 29.3 1.0 NE2 B:HIS118 1.9 47.1 1.0 CE1 B:HIS118 2.8 45.6 1.0 SG B:CYS115 2.9 81.1 1.0 CD2 B:HIS118 2.9 43.8 1.0 CB B:CYS115 3.3 37.1 1.0 ND1 B:HIS118 4.0 42.2 1.0 CG B:HIS118 4.0 41.6 1.0 O B:CYS115 4.2 42.0 1.0 CG2 B:VAL120 4.3 46.9 1.0 CA B:CYS115 4.5 39.1 1.0 C B:CYS115 4.6 41.5 1.0 CD2 B:LEU15 5.0 37.2 1.0

Zinc binding site 3 out of 6 in the Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn302 b:43.9 occ:1.00 NE2 H:HIS118 2.0 38.4 1.0 OD1 B:ASP102 2.0 49.2 1.0 O H:HOH406 2.5 28.8 1.0 CE1 H:HIS118 2.7 39.7 1.0 CG B:ASP102 2.8 45.6 1.0 SG H:CYS115 3.0 62.0 1.0 OD2 B:ASP102 3.1 44.9 1.0 CD2 H:HIS118 3.1 36.3 1.0 CB H:CYS115 3.6 38.1 1.0 ND1 H:HIS118 3.9 36.6 1.0 CG H:HIS118 4.1 36.6 1.0 CB B:ASP102 4.2 42.3 1.0 O H:CYS115 4.5 36.0 1.0 NZ B:LYS101 4.6 47.7 1.0 CA B:ASP102 4.7 40.6 1.0 CG2 H:VAL120 4.7 34.0 1.0 CA H:CYS115 4.9 36.2 1.0 C H:CYS115 4.9 38.2 1.0

Zinc binding site 4 out of 6 in the Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn301 b:43.0 occ:1.00 NE2 C:HIS118 1.8 44.0 1.0 SG C:CYS115 2.7 84.2 1.0 CE1 C:HIS118 2.7 42.8 1.0 CD2 C:HIS118 2.9 38.5 1.0 CB C:CYS115 3.0 43.3 1.0 O C:CYS115 3.8 46.1 1.0 ND1 C:HIS118 3.9 42.0 1.0 CG C:HIS118 4.0 39.5 1.0 CA C:CYS115 4.2 43.4 1.0 C C:CYS115 4.3 45.1 1.0

Zinc binding site 5 out of 6 in the Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn301 b:42.1 occ:1.00 NE2 D:HIS118 2.0 32.2 1.0 O D:HOH401 2.8 45.6 1.0 CE1 D:HIS118 3.0 35.1 1.0 CD2 D:HIS118 3.0 31.7 1.0 SG D:CYS115 3.3 67.5 1.0 CB D:CYS115 3.3 31.3 1.0 CG2 D:VAL120 3.6 45.3 1.0 CD1 D:LEU15 4.1 41.2 1.0 ND1 D:HIS118 4.1 38.6 1.0 CG D:HIS118 4.2 34.2 1.0 CA D:CYS115 4.8 42.6 1.0 CB D:VAL120 4.8 42.1 1.0 O D:CYS115 4.9 45.1 1.0 CG1 D:VAL120 5.0 42.5 1.0

Zinc binding site 6 out of 6 in the Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Crystal Structure of Full-Length Split Gfp Mutant E115C/T118H with Metal Mediated Crystal Contacts, P 1 21 1 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn301 b:42.8 occ:1.00 NE2 E:HIS118 1.9 35.5 1.0 CE1 E:HIS118 2.3 36.7 1.0 O E:HOH407 2.3 37.6 1.0 SG E:CYS115 2.9 68.0 1.0 CD2 E:HIS118 3.2 34.5 1.0 CB E:CYS115 3.5 36.8 1.0 ND1 E:HIS118 3.6 33.8 1.0 CG E:HIS118 4.0 35.0 1.0 O E:CYS115 4.5 38.8 1.0 CA E:CYS115 4.8 35.4 1.0 CG2 E:VAL120 4.8 37.4 1.0 C E:CYS115 4.8 40.4 1.0

D.J.Leibly, M.A.Arbing, I.Pashkov, N.Devore, G.S.Waldo, T.C.Terwilliger, T.O.Yeates. Engineering Novel Oligomeric Gfp Molecules For Synthetic Symmetrization Applications To Be Published.