Zinc in PDB 4ogc: Crystal Structure of the Type II-C CAS9 Enzyme From Actinomyces Naeslundii

The structure of Crystal Structure of the Type II-C CAS9 Enzyme From Actinomyces Naeslundii, PDB code: 4ogc was solved by F.Jiang, E.Ma, S.Lin, J.A.Doudna, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	68.30 / 2.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	74.610, 132.560, 80.040, 90.00, 95.38, 90.00
R / Rfree (%)	19.4 / 23.3

The structure of Crystal Structure of the Type II-C CAS9 Enzyme From Actinomyces Naeslundii also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Manganese	(Mn)	2 atoms

The binding sites of Zinc atom in the Crystal Structure of the Type II-C CAS9 Enzyme From Actinomyces Naeslundii (pdb code 4ogc). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of the Type II-C CAS9 Enzyme From Actinomyces Naeslundii, PDB code: 4ogc:

Zinc binding site 1 out of 1 in the Crystal Structure of the Type II-C CAS9 Enzyme From Actinomyces Naeslundii


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Type II-C CAS9 Enzyme From Actinomyces Naeslundii within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1201 b:56.3 occ:1.00 SG A:CYS605 2.2 56.3 1.0 SG A:CYS602 2.2 66.3 1.0 SG A:CYS566 2.3 53.8 1.0 SG A:CYS569 2.3 50.5 1.0 CB A:CYS605 3.1 43.5 1.0 CB A:CYS566 3.2 46.7 1.0 CB A:CYS569 3.3 44.2 1.0 CB A:CYS602 3.3 42.9 1.0 NH1 A:ARG604 3.7 85.7 1.0 N A:CYS602 3.9 48.4 1.0 N A:CYS569 3.9 55.1 1.0 CG2 A:THR571 4.1 57.5 1.0 CA A:CYS602 4.2 48.6 1.0 CA A:CYS569 4.2 53.3 1.0 N A:CYS605 4.2 57.2 1.0 CA A:CYS605 4.2 51.5 1.0 CD A:ARG604 4.5 76.0 1.0 CG1 A:VAL601 4.6 60.0 1.0 CB A:TYR568 4.6 56.0 1.0 CA A:CYS566 4.7 49.0 1.0 C A:CYS602 4.8 52.3 1.0 C A:TYR568 4.8 55.8 1.0 O A:CYS602 4.8 42.1 1.0 CZ A:ARG604 4.8 91.1 1.0 C A:CYS569 4.9 52.3 1.0 N A:GLY570 4.9 54.8 1.0

M.Jinek, F.Jiang, D.W.Taylor, S.H.Sternberg, E.Kaya, E.Ma, C.Anders, M.Hauer, K.Zhou, S.Lin, M.Kaplan, A.T.Iavarone, E.Charpentier, E.Nogales, J.A.Doudna. Structures of CAS9 Endonucleases Reveal Rna-Mediated Conformational Activation. Science V. 343 47997 2014.
ISSN: ISSN 0036-8075
PubMed: 24505130
DOI: 10.1126/SCIENCE.1247997