Zinc in PDB 4nei: ALG17C PL17 Family Alginate Lyase

The structure of ALG17C PL17 Family Alginate Lyase, PDB code: 4nei was solved by D.S.Park, S.K.Nair, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 1.85
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	85.530, 126.351, 87.631, 90.00, 111.51, 90.00
R / Rfree (%)	16.8 / 19.2

The binding sites of Zinc atom in the ALG17C PL17 Family Alginate Lyase (pdb code 4nei). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the ALG17C PL17 Family Alginate Lyase, PDB code: 4nei:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the ALG17C PL17 Family Alginate Lyase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of ALG17C PL17 Family Alginate Lyase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn800 b:19.9 occ:1.00 O A:HOH1447 2.2 14.2 1.0 O A:HOH919 2.2 14.4 1.0 NE2 A:HIS464 2.2 15.0 1.0 O A:HOH933 2.2 13.7 1.0 ND1 A:HIS415 2.2 16.1 1.0 OD1 A:ASP433 2.3 15.0 1.0 CE1 A:HIS415 3.0 16.2 1.0 CD2 A:HIS464 3.0 14.8 1.0 CE1 A:HIS464 3.2 15.1 1.0 CG A:ASP433 3.2 15.5 1.0 CG A:HIS415 3.3 16.2 1.0 OD2 A:ASP433 3.4 15.4 1.0 CB A:HIS415 3.8 16.5 1.0 O A:HOH914 3.9 15.0 1.0 O A:ALA458 4.0 17.4 1.0 N A:ASN310 4.1 16.0 1.0 OD2 A:ASP417 4.1 17.3 1.0 NE2 A:HIS415 4.2 16.2 1.0 CG A:HIS464 4.2 14.8 1.0 O A:ASN310 4.3 15.8 1.0 ND1 A:HIS464 4.3 14.9 1.0 N A:GLY435 4.3 15.2 1.0 O A:GLY435 4.4 16.0 1.0 CD2 A:HIS415 4.4 16.4 1.0 O A:PRO308 4.5 16.0 1.0 CA A:ASN310 4.5 15.8 1.0 CB A:ASP433 4.6 15.3 1.0 CA A:GLY435 4.7 15.7 1.0 C A:ASN310 4.7 16.0 1.0 N A:TYR434 4.7 15.1 1.0 CA A:ILE309 4.8 16.1 1.0 C A:ILE309 4.8 16.0 1.0 C A:GLY435 4.9 16.0 1.0 CG A:ASP417 4.9 17.0 1.0

Zinc binding site 2 out of 2 in the ALG17C PL17 Family Alginate Lyase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of ALG17C PL17 Family Alginate Lyase within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn800 b:18.0 occ:1.00 NE2 B:HIS464 2.1 14.1 1.0 O B:HOH902 2.2 10.9 1.0 O B:HOH906 2.2 12.5 1.0 O B:HOH903 2.2 12.2 1.0 ND1 B:HIS415 2.2 14.1 1.0 OD1 B:ASP433 2.3 13.6 1.0 CE1 B:HIS415 3.0 14.2 1.0 CD2 B:HIS464 3.0 14.3 1.0 CG B:ASP433 3.2 13.8 1.0 CE1 B:HIS464 3.2 14.4 1.0 CG B:HIS415 3.4 14.4 1.0 OD2 B:ASP433 3.4 13.7 1.0 CB B:HIS415 3.9 14.5 1.0 O B:ALA458 4.0 14.4 1.0 O B:HOH937 4.0 13.8 1.0 N B:ASN310 4.1 14.5 1.0 NE2 B:HIS415 4.2 14.3 1.0 OD2 B:ASP417 4.2 16.9 1.0 CG B:HIS464 4.2 14.3 1.0 ND1 B:HIS464 4.3 14.7 1.0 O B:ASN310 4.3 14.8 1.0 O B:GLY435 4.3 15.0 1.0 N B:GLY435 4.3 14.5 1.0 CD2 B:HIS415 4.4 14.2 1.0 O B:PRO308 4.4 14.4 1.0 CA B:ASN310 4.6 14.7 1.0 CB B:ASP433 4.6 13.8 1.0 N B:TYR434 4.7 14.1 1.0 CA B:GLY435 4.7 14.7 1.0 C B:ASN310 4.7 14.9 1.0 CA B:ILE309 4.8 14.1 1.0 C B:ILE309 4.8 14.5 1.0 C B:GLY435 4.9 14.9 1.0 CG B:ASP417 4.9 16.9 1.0

D.Park, S.Jagtap, S.K.Nair. Structure of A PL17 Family Alginate Lyase Demonstrates Functional Similarities Among Exotype Depolymerases. J.Biol.Chem. V. 289 8645 2014.
ISSN: ISSN 0021-9258
PubMed: 24478312
DOI: 10.1074/JBC.M113.531111