Zinc in PDB 4n0n: Crystal Structure of Arterivirus Nonstructural Protein 10 (Helicase)

All present enzymatic activity of Crystal Structure of Arterivirus Nonstructural Protein 10 (Helicase):
2.7.7.48; 3.4.19.12; 3.6.4.12; 3.6.4.13;

The structure of Crystal Structure of Arterivirus Nonstructural Protein 10 (Helicase), PDB code: 4n0n was solved by Z.Deng, Z.Chen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.00
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	89.763, 91.041, 57.739, 90.00, 90.00, 90.00
R / Rfree (%)	19.5 / 22.4

The structure of Crystal Structure of Arterivirus Nonstructural Protein 10 (Helicase) also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Crystal Structure of Arterivirus Nonstructural Protein 10 (Helicase) (pdb code 4n0n). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of Arterivirus Nonstructural Protein 10 (Helicase), PDB code: 4n0n:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of Arterivirus Nonstructural Protein 10 (Helicase)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Arterivirus Nonstructural Protein 10 (Helicase) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:31.0 occ:1.00 ND1 A:HIS32 2.0 32.8 1.0 NE2 A:HIS29 2.1 31.1 1.0 SG A:CYS33 2.2 31.0 1.0 SG A:CYS17 2.3 32.2 1.0 CE1 A:HIS32 2.9 34.0 1.0 CE1 A:HIS29 3.1 31.0 1.0 CD2 A:HIS29 3.1 30.4 1.0 CG A:HIS32 3.1 32.4 1.0 CB A:CYS17 3.2 31.9 1.0 CB A:CYS33 3.4 34.0 1.0 CB A:HIS32 3.6 32.2 1.0 N A:CYS33 3.8 33.1 1.0 OG A:SER15 3.9 33.2 1.0 CB A:SER15 4.0 33.3 1.0 N A:CYS17 4.0 33.1 1.0 NE2 A:HIS32 4.1 32.1 1.0 CA A:CYS33 4.1 33.4 1.0 C A:HIS32 4.2 33.6 1.0 CD2 A:HIS32 4.2 32.5 1.0 ND1 A:HIS29 4.2 32.1 1.0 CA A:CYS17 4.2 32.2 1.0 CG A:HIS29 4.3 31.0 1.0 CD2 A:LEU76 4.3 29.6 1.0 CA A:HIS32 4.6 32.8 1.0 C A:CYS33 4.7 34.9 1.0 O A:HIS32 4.8 33.5 1.0 O A:CYS33 4.8 33.6 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of Arterivirus Nonstructural Protein 10 (Helicase)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Arterivirus Nonstructural Protein 10 (Helicase) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn502 b:47.0 occ:0.80 ND1 A:HIS44 2.0 49.7 1.0 SG A:CYS53 2.3 52.5 1.0 SG A:CYS42 2.3 61.3 1.0 SG A:CYS56 2.5 59.2 1.0 CE1 A:HIS44 2.9 54.6 1.0 CG A:HIS44 3.1 52.9 1.0 CB A:CYS53 3.1 55.6 1.0 CB A:CYS42 3.3 64.1 1.0 CB A:CYS56 3.4 61.7 1.0 CB A:HIS44 3.5 49.9 1.0 N A:CYS56 3.6 57.0 1.0 CA A:CYS56 4.0 61.0 1.0 NE2 A:HIS44 4.1 54.3 1.0 CD2 A:HIS44 4.2 52.7 1.0 O A:HOH697 4.3 44.8 1.0 CB A:MET55 4.4 56.5 1.0 C A:MET55 4.5 56.5 1.0 CA A:CYS53 4.6 55.9 1.0 NH2 A:ARG49 4.6 65.1 1.0 CA A:CYS42 4.7 66.1 1.0 CA A:HIS44 4.8 50.0 1.0 N A:HIS44 4.8 52.9 1.0 CA A:MET55 4.9 56.6 1.0 O A:CYS53 4.9 55.1 1.0 C A:CYS53 5.0 55.1 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of Arterivirus Nonstructural Protein 10 (Helicase)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Arterivirus Nonstructural Protein 10 (Helicase) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn503 b:33.1 occ:1.00 SG A:CYS25 2.3 31.8 1.0 SG A:CYS7 2.3 35.9 1.0 SG A:CYS22 2.4 34.0 1.0 SG A:CYS4 2.4 34.5 1.0 CB A:CYS25 3.1 30.5 1.0 CB A:CYS4 3.3 34.8 1.0 CB A:CYS7 3.4 36.8 1.0 CB A:CYS22 3.6 32.9 1.0 N A:CYS7 3.7 35.8 1.0 O A:HOH762 4.0 46.6 1.0 CA A:CYS7 4.1 38.1 1.0 N A:CYS22 4.1 32.1 1.0 N A:CYS25 4.2 32.4 1.0 CA A:CYS25 4.3 31.2 1.0 CA A:CYS22 4.4 33.2 1.0 CB A:VAL6 4.5 36.0 1.0 O A:HOH724 4.6 41.7 1.0 CB A:ALA9 4.7 40.1 1.0 CA A:CYS4 4.8 37.1 1.0 N A:GLY8 4.8 39.2 1.0 C A:CYS7 4.8 39.4 1.0 C A:VAL6 4.8 35.4 1.0 CB A:ASN24 4.8 35.5 1.0 N A:ALA9 4.8 40.2 1.0 C A:CYS22 4.9 33.5 1.0 O A:CYS22 4.9 32.8 1.0 CA A:VAL6 5.0 35.2 1.0

Z.Deng, K.C.Lehmann, X.Li, C.Feng, G.Wang, Q.Zhang, X.Qi, L.Yu, X.Zhang, W.Feng, W.Wu, P.Gong, Y.Tao, C.C.Posthuma, E.J.Snijder, A.E.Gorbalenya, Z.Chen. Structural Basis For the Regulatory Function of A Complex Zinc-Binding Domain in A Replicative Arterivirus Helicase Resembling A Nonsense-Mediated Mrna Decay Helicase. Nucleic Acids Res. V. 42 3464 2014.
ISSN: ISSN 0305-1048
PubMed: 24369429
DOI: 10.1093/NAR/GKT1310