Zinc in PDB 4k4s: Poliovirus Polymerase Elongation Complex (R3_FORM)

All present enzymatic activity of Poliovirus Polymerase Elongation Complex (R3_FORM):
2.7.7.48;

The structure of Poliovirus Polymerase Elongation Complex (R3_FORM), PDB code: 4k4s was solved by P.Gong, O.B.Peersen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.92 / 2.40
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	58.768, 58.859, 97.586, 77.88, 77.80, 79.97
R / Rfree (%)	19.8 / 24.5

The binding sites of Zinc atom in the Poliovirus Polymerase Elongation Complex (R3_FORM) (pdb code 4k4s). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Poliovirus Polymerase Elongation Complex (R3_FORM), PDB code: 4k4s:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Poliovirus Polymerase Elongation Complex (R3_FORM)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Poliovirus Polymerase Elongation Complex (R3_FORM) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:44.3 occ:1.00 NE2 A:HIS272 2.1 56.8 1.0 CE1 A:HIS272 2.3 60.6 1.0 SG A:CYS281 2.4 56.0 1.0 NE2 A:HIS270 2.8 65.6 1.0 CE1 A:HIS270 2.8 61.3 1.0 CD2 A:HIS272 3.3 58.1 1.0 CB A:CYS281 3.4 54.2 1.0 ND1 A:HIS272 3.5 64.1 1.0 ND1 A:HIS270 3.9 68.3 1.0 CD2 A:HIS270 3.9 66.7 1.0 CG A:HIS272 3.9 50.4 1.0 NE1 A:TRP5 4.2 56.9 1.0 CG A:HIS270 4.5 61.4 1.0 CA A:CYS281 4.6 47.9 1.0 CZ2 A:TRP5 4.6 54.9 1.0 CE2 A:TRP5 4.7 53.8 1.0

Zinc binding site 2 out of 2 in the Poliovirus Polymerase Elongation Complex (R3_FORM)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Poliovirus Polymerase Elongation Complex (R3_FORM) within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn501 b:42.9 occ:1.00 NE2 E:HIS272 2.0 52.7 1.0 CE1 E:HIS272 2.3 58.2 1.0 SG E:CYS281 2.4 56.5 1.0 CE1 E:HIS270 2.7 63.1 1.0 NE2 E:HIS270 2.8 66.8 1.0 CD2 E:HIS272 3.2 55.6 1.0 CB E:CYS281 3.4 50.0 1.0 ND1 E:HIS272 3.5 64.3 1.0 ND1 E:HIS270 3.8 65.8 1.0 CD2 E:HIS270 3.9 64.8 1.0 CG E:HIS272 3.9 48.4 1.0 NE1 E:TRP5 4.2 54.7 1.0 CG E:HIS270 4.4 61.7 1.0 CA E:CYS281 4.6 48.2 1.0 O E:HOH644 4.6 61.0 1.0 CZ2 E:TRP5 4.7 52.1 1.0 CE2 E:TRP5 4.7 50.4 1.0

P.Gong, M.G.Kortus, J.C.Nix, R.E.Davis, O.B.Peersen. Structures of Coxsackievirus, Rhinovirus, and Poliovirus Polymerase Elongation Complexes Solved By Engineering Rna Mediated Crystal Contacts. Plos One V. 8 60272 2013.
ISSN: ESSN 1932-6203
PubMed: 23667424
DOI: 10.1371/JOURNAL.PONE.0060272