Zinc in PDB 4i68: Crystal Structure of the R444A / R449A Double Mutant of the Hera Rna Helicase Rrm Domain

All present enzymatic activity of Crystal Structure of the R444A / R449A Double Mutant of the Hera Rna Helicase Rrm Domain:
3.6.4.13;

The structure of Crystal Structure of the R444A / R449A Double Mutant of the Hera Rna Helicase Rrm Domain, PDB code: 4i68 was solved by M.G.Rudolph, D.Klostermeier, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.43 / 1.63
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	25.160, 46.030, 59.610, 90.00, 90.00, 90.00
R / Rfree (%)	17.9 / 23.1

The structure of Crystal Structure of the R444A / R449A Double Mutant of the Hera Rna Helicase Rrm Domain also contains other interesting chemical elements:

Chlorine

(Cl)

3 atoms

The binding sites of Zinc atom in the Crystal Structure of the R444A / R449A Double Mutant of the Hera Rna Helicase Rrm Domain (pdb code 4i68). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of the R444A / R449A Double Mutant of the Hera Rna Helicase Rrm Domain, PDB code: 4i68:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of the R444A / R449A Double Mutant of the Hera Rna Helicase Rrm Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the R444A / R449A Double Mutant of the Hera Rna Helicase Rrm Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn604 b:12.5 occ:1.00 OE1 A:GLU425 2.0 15.1 1.0 O A:HOH713 2.1 18.1 1.0 O A:HOH774 2.2 25.1 1.0 OE2 A:GLU425 2.7 15.3 1.0 CD A:GLU425 2.7 11.2 1.0 NE A:ARG436 3.9 9.1 1.0 CG A:GLU425 4.1 8.6 1.0 O A:HOH712 4.2 9.7 1.0 O A:HOH701 4.4 7.1 1.0 NH2 A:ARG436 4.4 7.1 1.0 CZ A:ARG436 4.6 10.3 1.0 CD A:ARG436 4.8 4.8 1.0 CG A:ARG436 4.9 4.6 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of the R444A / R449A Double Mutant of the Hera Rna Helicase Rrm Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the R444A / R449A Double Mutant of the Hera Rna Helicase Rrm Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn605 b:20.3 occ:1.00 O A:HOH744 2.0 19.3 1.0 OE1 A:GLU499 2.1 19.6 1.0 CD A:GLU499 2.9 20.1 1.0 OE2 A:GLU499 3.0 17.5 1.0 O A:HOH773 3.5 44.1 1.0 O A:HOH797 4.0 26.8 1.0 O A:HOH760 4.1 22.4 1.0 CD2 A:LEU447 4.2 25.3 1.0 CG A:GLU499 4.3 15.0 1.0 OE1 A:GLU466 4.6 32.1 1.0 CB A:GLU499 4.7 16.3 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of the R444A / R449A Double Mutant of the Hera Rna Helicase Rrm Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of the R444A / R449A Double Mutant of the Hera Rna Helicase Rrm Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn606 b:32.7 occ:1.00 OE1 A:GLU432 2.2 15.7 1.0 O A:HOH729 2.4 17.8 1.0 OE2 A:GLU432 2.7 28.0 1.0 CD A:GLU432 2.8 18.6 1.0 O A:HOH752 3.2 20.2 1.0 NE1 A:TRP435 3.6 8.8 1.0 CG A:GLU432 4.2 10.0 1.0 O A:HOH709 4.2 10.9 1.0 O A:HOH764 4.4 22.7 1.0 NH2 A:ARG503 4.4 40.2 1.0 CE2 A:TRP435 4.4 8.7 1.0 CD1 A:TRP435 4.5 7.8 1.0 CZ2 A:TRP435 4.6 10.3 1.0 O A:HOH787 4.7 30.7 1.0 CB A:GLU432 5.0 6.4 1.0

L.Steimer, J.P.Wurm, M.H.Linden, M.G.Rudolph, J.Wohnert, D.Klostermeier. Recognition of Two Distinct Elements in the Rna Substrate By the Rna-Binding Domain of the T. Thermophilus Dead Box Helicase Hera. Nucleic Acids Res. V. 41 6259 2013.
ISSN: ISSN 0305-1048
PubMed: 23625962
DOI: 10.1093/NAR/GKT323