Zinc in PDB 4i1s: Melanoma Differentiation Associated Protein-5 Helicase Domain Complex with Inhibitor Non-Structural Protein V

The structure of Melanoma Differentiation Associated Protein-5 Helicase Domain Complex with Inhibitor Non-Structural Protein V, PDB code: 4i1s was solved by C.Motz, G.Witte, K.P.Hopfner, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.14 / 2.29
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.070, 52.490, 120.790, 90.00, 90.00, 90.00
R / Rfree (%)	17.9 / 23

The binding sites of Zinc atom in the Melanoma Differentiation Associated Protein-5 Helicase Domain Complex with Inhibitor Non-Structural Protein V (pdb code 4i1s). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Melanoma Differentiation Associated Protein-5 Helicase Domain Complex with Inhibitor Non-Structural Protein V, PDB code: 4i1s:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Melanoma Differentiation Associated Protein-5 Helicase Domain Complex with Inhibitor Non-Structural Protein V


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Melanoma Differentiation Associated Protein-5 Helicase Domain Complex with Inhibitor Non-Structural Protein V within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn301 b:54.1 occ:1.00 SG B:CYS211 2.3 57.3 1.0 SG B:CYS194 2.3 52.8 1.0 SG B:CYS206 2.4 60.9 1.0 SG B:CYS208 2.4 56.4 1.0 CB B:CYS211 3.1 72.8 1.0 CB B:CYS194 3.1 56.5 1.0 CB B:CYS206 3.4 54.3 1.0 CB B:CYS208 3.5 47.7 1.0 N B:CYS208 3.6 58.1 1.0 N B:CYS211 3.9 72.5 1.0 CA B:CYS206 3.9 52.9 1.0 CB B:PRO192 3.9 45.3 1.0 CA B:CYS208 4.0 57.5 1.0 N B:HIS209 4.0 72.2 1.0 N B:THR207 4.0 58.0 1.0 N B:CYS194 4.1 48.6 1.0 CA B:CYS211 4.1 73.4 1.0 CA B:CYS194 4.2 50.7 1.0 C B:CYS206 4.3 57.8 1.0 C B:CYS208 4.3 68.5 1.0 CA B:GLY168 4.5 67.1 1.0 N B:GLN210 4.6 73.7 1.0 C B:THR207 4.7 60.6 1.0 N B:GLY169 4.7 72.3 1.0 N B:SER193 4.8 49.1 1.0 CA B:PRO192 4.9 47.9 1.0 C B:PRO192 4.9 49.8 1.0 C B:GLN210 4.9 74.7 1.0 CA B:THR207 5.0 56.5 1.0 CA B:HIS209 5.0 73.8 1.0

Zinc binding site 2 out of 2 in the Melanoma Differentiation Associated Protein-5 Helicase Domain Complex with Inhibitor Non-Structural Protein V


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Melanoma Differentiation Associated Protein-5 Helicase Domain Complex with Inhibitor Non-Structural Protein V within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn302 b:48.1 occ:1.00 ND1 B:HIS171 2.0 48.1 1.0 SG B:CYS218 2.2 52.0 1.0 SG B:CYS215 2.2 59.6 1.0 SG B:CYS190 2.4 45.4 1.0 CE1 B:HIS171 2.8 50.8 1.0 CG B:HIS171 3.1 51.0 1.0 CB B:CYS190 3.1 33.6 1.0 CB B:CYS218 3.3 68.1 1.0 N B:CYS215 3.4 54.2 1.0 CB B:CYS215 3.4 56.0 1.0 CB B:HIS171 3.6 51.2 1.0 N B:CYS218 3.7 62.4 1.0 CA B:CYS215 3.9 54.1 1.0 NE2 B:HIS171 3.9 52.5 1.0 CD2 B:HIS171 4.1 52.0 1.0 CA B:CYS218 4.1 68.7 1.0 C B:THR214 4.3 64.3 1.0 C B:CYS215 4.5 52.4 1.0 O B:CYS215 4.5 50.1 1.0 C B:GLU217 4.6 58.8 1.0 CA B:CYS190 4.6 35.0 1.0 CA B:THR214 4.6 63.6 1.0 CB B:GLU217 4.7 49.2 1.0 NH1 B:ARG173 4.7 47.9 1.0 N B:GLU217 4.9 55.0 1.0 CA B:GLU217 4.9 55.8 1.0

C.Motz, K.M.Schuhmann, A.Kirchhofer, M.Moldt, G.Witte, K.K.Conzelmann, K.P.Hopfner. Paramyxovirus V Proteins Disrupt the Fold of the Rna Sensor MDA5 to Inhibit Antiviral Signaling. Science V. 339 690 2013.
ISSN: ISSN 0036-8075
PubMed: 23328395
DOI: 10.1126/SCIENCE.1230949