Zinc in PDB 4h45: Product Complexes of Porcine Liver Fructose-1,6-Bisphosphatase with Mutation E192Q

All present enzymatic activity of Product Complexes of Porcine Liver Fructose-1,6-Bisphosphatase with Mutation E192Q:
3.1.3.11;

The structure of Product Complexes of Porcine Liver Fructose-1,6-Bisphosphatase with Mutation E192Q, PDB code: 4h45 was solved by Y.Gao, R.B.Honzatko, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.42 / 3.10
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	53.996, 83.621, 166.220, 90.00, 90.00, 90.00
R / Rfree (%)	22.7 / 28.3

The binding sites of Zinc atom in the Product Complexes of Porcine Liver Fructose-1,6-Bisphosphatase with Mutation E192Q (pdb code 4h45). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Product Complexes of Porcine Liver Fructose-1,6-Bisphosphatase with Mutation E192Q, PDB code: 4h45:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Product Complexes of Porcine Liver Fructose-1,6-Bisphosphatase with Mutation E192Q


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Product Complexes of Porcine Liver Fructose-1,6-Bisphosphatase with Mutation E192Q within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn402 b:0.9 occ:1.00 OD2 A:ASP118 2.1 41.4 1.0 O A:LEU120 2.1 42.2 1.0 O2 A:PO4404 2.6 78.9 1.0 C A:LEU120 3.0 42.1 1.0 CG A:ASP118 3.0 39.0 1.0 OD1 A:ASP118 3.2 39.0 1.0 N A:LEU120 3.6 40.0 1.0 OE2 A:GLU97 3.6 59.6 1.0 O4 A:PO4404 3.7 78.0 1.0 P A:PO4404 3.7 78.5 1.0 N A:ASP121 3.7 43.8 1.0 CA A:ASP121 3.8 45.7 1.0 CD A:GLU97 3.8 58.2 1.0 CA A:LEU120 3.9 41.0 1.0 CD A:PRO119 3.9 37.7 1.0 CG A:GLU97 4.0 55.2 1.0 ZN A:ZN403 4.0 0.2 1.0 CB A:GLU97 4.1 52.9 1.0 OD1 A:ASP74 4.1 74.4 1.0 CG A:PRO119 4.2 37.2 1.0 CB A:ASP118 4.3 38.3 1.0 O3 A:PO4404 4.3 78.8 1.0 N A:PRO119 4.4 37.9 1.0 OE2 A:GLU98 4.4 56.5 1.0 C A:ASP118 4.5 38.1 1.0 CB A:LEU120 4.5 40.5 1.0 OE1 A:GLU97 4.6 58.2 1.0 CB A:ASP121 4.6 46.0 1.0 CA A:ASP118 4.6 38.5 1.0 C A:PRO119 4.7 38.8 1.0 O1 A:PO4404 4.9 78.0 1.0 C A:ASP121 4.9 47.0 1.0

Zinc binding site 2 out of 2 in the Product Complexes of Porcine Liver Fructose-1,6-Bisphosphatase with Mutation E192Q


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Product Complexes of Porcine Liver Fructose-1,6-Bisphosphatase with Mutation E192Q within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn403 b:0.2 occ:1.00 O2 A:PO4404 2.0 78.9 1.0 OD1 A:ASP118 2.2 39.0 1.0 OE1 A:GLU280 2.4 38.5 1.0 P A:PO4404 2.9 78.5 1.0 CB A:ASP121 3.0 46.0 1.0 O3 A:PO4404 3.1 78.8 1.0 OD1 A:ASP121 3.2 48.8 1.0 O1 A:PO4404 3.2 78.0 1.0 C1 A:F6P401 3.4 35.3 1.0 CA A:ASP121 3.4 45.7 1.0 CG A:ASP118 3.4 39.0 1.0 CG A:ASP121 3.4 47.9 1.0 CD A:GLU280 3.5 38.0 1.0 NH2 A:ARG276 3.6 58.3 1.0 OE2 A:GLU97 3.7 59.6 1.0 ZN A:ZN402 4.0 0.9 1.0 OD2 A:ASP118 4.0 41.4 1.0 N A:GLY122 4.1 48.0 1.0 O1 A:F6P401 4.2 38.6 1.0 CG A:GLU280 4.2 36.3 1.0 C A:ASP121 4.3 47.0 1.0 O4 A:PO4404 4.3 78.0 1.0 OE2 A:GLU280 4.3 39.7 1.0 CD A:GLU97 4.4 58.2 1.0 OE1 A:GLU97 4.5 58.2 1.0 N A:ASP121 4.5 43.8 1.0 OD2 A:ASP121 4.5 49.0 1.0 CB A:ASP118 4.6 38.3 1.0 C2 A:F6P401 4.6 34.6 1.0 O A:LEU120 4.7 42.2 1.0 O3 A:F6P401 4.8 34.6 1.0 CZ A:ARG276 4.8 58.3 1.0 O2 A:F6P401 4.9 35.8 1.0

Y.Gao, R.B.Honzatko. Dimer-Dimer Interface in Porcine Liver Fructose-1,6-Bisphosphatase Is Essential in Cooperative Binding of Amp To Be Published.