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Zinc in PDB 4gzz: Crystal Structures of Bacterial Rna Polymerase Paused Elongation Complexes

Enzymatic activity of Crystal Structures of Bacterial Rna Polymerase Paused Elongation Complexes

All present enzymatic activity of Crystal Structures of Bacterial Rna Polymerase Paused Elongation Complexes:
2.7.7.6;

Protein crystallography data

The structure of Crystal Structures of Bacterial Rna Polymerase Paused Elongation Complexes, PDB code: 4gzz was solved by A.Weixlbaumer, K.Leon, R.Landick, S.A.Darst, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 38.79 / 4.29
Space group H 3
Cell size a, b, c (Å), α, β, γ (°) 286.549, 286.549, 199.411, 90.00, 90.00, 120.00
R / Rfree (%) 23.5 / 28.5

Other elements in 4gzz:

The structure of Crystal Structures of Bacterial Rna Polymerase Paused Elongation Complexes also contains other interesting chemical elements:

Magnesium (Mg) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structures of Bacterial Rna Polymerase Paused Elongation Complexes (pdb code 4gzz). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structures of Bacterial Rna Polymerase Paused Elongation Complexes, PDB code: 4gzz:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 4gzz

Go back to Zinc Binding Sites List in 4gzz
Zinc binding site 1 out of 2 in the Crystal Structures of Bacterial Rna Polymerase Paused Elongation Complexes


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structures of Bacterial Rna Polymerase Paused Elongation Complexes within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn1601

b:70.0
occ:1.00
SG D:CYS73 2.7 0.8 1.0
SG D:CYS58 3.4 0.9 1.0
CB D:CYS73 3.4 0.9 1.0
SG D:CYS60 3.4 0.2 1.0
SG D:CYS76 3.5 0.8 1.0
CB D:LYS62 3.5 0.9 1.0
CG D:LYS62 3.6 0.2 1.0
N D:LYS62 3.8 0.2 1.0
CA D:LYS62 4.2 0.4 1.0
N D:GLY61 4.3 0.3 1.0
CB D:CYS60 4.4 0.6 1.0
O D:CYS76 4.5 0.2 1.0
CB D:CYS58 4.6 0.5 1.0
CD1 D:TYR63 4.6 0.6 1.0
N D:CYS76 4.6 0.2 1.0
CB D:CYS76 4.6 0.1 1.0
CG D:ARG75 4.8 0.8 1.0
N D:TYR63 4.8 0.8 1.0
N D:CYS60 4.8 0.5 1.0
C D:GLY61 4.9 0.6 1.0
CA D:CYS73 4.9 0.5 1.0
CD D:LYS62 5.0 0.6 1.0

Zinc binding site 2 out of 2 in 4gzz

Go back to Zinc Binding Sites List in 4gzz
Zinc binding site 2 out of 2 in the Crystal Structures of Bacterial Rna Polymerase Paused Elongation Complexes


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structures of Bacterial Rna Polymerase Paused Elongation Complexes within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn1602

b:70.0
occ:1.00
SG D:CYS1204 2.3 80.0 1.0
SG D:CYS1112 2.6 0.6 1.0
SG D:CYS1201 2.6 81.1 1.0
SG D:CYS1194 2.7 0.9 1.0
CB D:CYS1204 3.2 76.0 1.0
CB D:CYS1194 3.3 0.0 1.0
CB D:CYS1112 3.3 0.1 1.0
CB D:CYS1201 3.5 80.3 1.0
CA D:CYS1194 3.6 83.5 1.0
N D:CYS1201 4.0 74.9 1.0
N D:GLN1195 4.1 0.0 1.0
CA D:CYS1201 4.4 75.6 1.0
C D:CYS1194 4.4 86.4 1.0
CA D:CYS1204 4.5 80.0 1.0
CA D:CYS1112 4.6 0.1 1.0
N D:CYS1112 4.6 0.8 1.0
N D:CYS1204 4.6 80.7 1.0
N D:CYS1194 4.7 82.8 1.0
CG2 D:THR1114 4.7 0.4 1.0

Reference:

A.Weixlbaumer, K.Leon, R.Landick, S.A.Darst. Structural Basis of Transcriptional Pausing in Bacteria. Cell(Cambridge,Mass.) V. 152 431 2013.
ISSN: ISSN 0092-8674
PubMed: 23374340
DOI: 10.1016/J.CELL.2012.12.020
Page generated: Wed Dec 16 05:21:22 2020

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