Zinc in PDB 4gx5: Gsuk Channel

The structure of Gsuk Channel, PDB code: 4gx5 was solved by C.Kong, W.Zeng, S.Ye, L.Chen, D.B.Sauer, Y.Lam, M.G.Derebe, Y.Jiang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.39 / 3.70
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	234.976, 108.364, 165.787, 90.00, 134.96, 90.00
R / Rfree (%)	26.1 / 29.3

The structure of Gsuk Channel also contains other interesting chemical elements:

Potassium	(K)	12 atoms
Calcium	(Ca)	4 atoms

The binding sites of Zinc atom in the Gsuk Channel (pdb code 4gx5). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Gsuk Channel, PDB code: 4gx5:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Gsuk Channel


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Gsuk Channel within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn605 b:0.0 occ:1.00 NE2 A:HIS359 2.5 0.4 1.0 ND1 A:HIS391 2.7 0.8 1.0 SG A:CYS364 2.8 0.3 1.0 SG A:CYS388 2.8 0.1 1.0 CG A:HIS391 3.0 0.9 1.0 CB A:HIS391 3.1 0.8 1.0 CB A:CYS364 3.1 0.4 1.0 CB A:CYS388 3.2 0.7 1.0 CE1 A:HIS359 3.5 0.4 1.0 CD2 A:HIS359 3.5 0.1 1.0 CE1 A:HIS391 3.6 0.4 1.0 CA A:CYS364 3.8 0.7 1.0 CD2 A:HIS391 4.0 1.0 1.0 NE2 A:HIS391 4.3 0.8 1.0 CD1 A:LEU379 4.4 0.6 1.0 CA A:HIS391 4.4 0.7 1.0 O A:CYS364 4.4 0.2 1.0 N A:HIS391 4.5 0.8 1.0 C A:CYS364 4.5 0.4 1.0 ND1 A:HIS359 4.6 0.3 1.0 CG A:HIS359 4.6 0.6 1.0 CA A:CYS388 4.7 0.4 1.0 N A:CYS364 5.0 0.9 1.0

Zinc binding site 2 out of 4 in the Gsuk Channel


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Gsuk Channel within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn601 b:0.7 occ:1.00 NE2 B:HIS359 2.5 0.0 1.0 SG B:CYS364 2.7 0.1 1.0 ND1 B:HIS391 2.7 0.5 1.0 SG B:CYS388 2.9 0.6 1.0 CB B:CYS364 3.0 0.6 1.0 CG B:HIS391 3.1 0.2 1.0 CB B:HIS391 3.2 1.0 1.0 CB B:CYS388 3.3 0.6 1.0 CD2 B:HIS359 3.4 0.2 1.0 CE1 B:HIS359 3.5 0.4 1.0 CE1 B:HIS391 3.6 0.1 1.0 CA B:CYS364 3.6 0.7 1.0 CD2 B:HIS391 4.0 0.1 1.0 NE2 B:HIS391 4.3 0.7 1.0 O B:CYS364 4.3 0.8 1.0 CD1 B:LEU379 4.3 0.9 1.0 C B:CYS364 4.4 0.8 1.0 CA B:HIS391 4.5 0.2 1.0 CG B:HIS359 4.6 0.9 1.0 ND1 B:HIS359 4.6 1.0 1.0 N B:HIS391 4.6 0.7 1.0 CA B:CYS388 4.8 0.2 1.0 N B:CYS364 4.9 0.6 1.0

Zinc binding site 3 out of 4 in the Gsuk Channel


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Gsuk Channel within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn605 b:0.1 occ:1.00 NE2 C:HIS359 2.5 0.0 1.0 ND1 C:HIS391 2.7 0.6 1.0 SG C:CYS364 2.8 0.9 1.0 CG C:HIS391 3.0 0.4 1.0 CB C:CYS364 3.0 0.7 1.0 SG C:CYS388 3.0 0.0 1.0 CB C:HIS391 3.1 0.7 1.0 CD2 C:HIS359 3.3 0.7 1.0 CB C:CYS388 3.4 0.8 1.0 CE1 C:HIS359 3.5 0.2 1.0 CE1 C:HIS391 3.6 0.3 1.0 CA C:CYS364 3.6 0.8 1.0 CD2 C:HIS391 3.9 0.7 1.0 NE2 C:HIS391 4.2 0.8 1.0 CD1 C:LEU379 4.2 0.3 1.0 O C:CYS364 4.3 0.7 1.0 C C:CYS364 4.3 1.0 1.0 CA C:HIS391 4.5 0.8 1.0 CG C:HIS359 4.5 0.7 1.0 ND1 C:HIS359 4.6 0.6 1.0 N C:HIS391 4.7 0.5 1.0 N C:CYS364 4.8 0.6 1.0 CA C:CYS388 4.9 0.1 1.0

Zinc binding site 4 out of 4 in the Gsuk Channel


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Gsuk Channel within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn601 b:0.6 occ:1.00 NE2 D:HIS359 2.6 0.1 1.0 ND1 D:HIS391 2.7 0.1 1.0 SG D:CYS388 2.7 0.5 1.0 SG D:CYS364 2.8 0.1 1.0 CG D:HIS391 3.0 1.0 1.0 CB D:CYS388 3.1 0.8 1.0 CB D:HIS391 3.1 0.3 1.0 CB D:CYS364 3.2 0.1 1.0 CE1 D:HIS359 3.5 0.4 1.0 CD2 D:HIS359 3.6 0.8 1.0 CE1 D:HIS391 3.6 0.8 1.0 CA D:CYS364 3.9 0.3 1.0 CD2 D:HIS391 4.1 0.3 1.0 NE2 D:HIS391 4.3 0.9 1.0 CA D:HIS391 4.4 0.3 1.0 N D:HIS391 4.5 0.5 1.0 O D:CYS364 4.5 0.7 1.0 CD1 D:LEU379 4.5 0.7 1.0 C D:CYS364 4.6 0.8 1.0 CA D:CYS388 4.6 0.7 1.0 ND1 D:HIS359 4.6 0.2 1.0 CG D:HIS359 4.7 0.2 1.0

C.Kong, W.Zeng, S.Ye, L.Chen, D.B.Sauer, Y.Lam, M.G.Derebe, Y.Jiang. Distinct Gating Mechanisms Revealed By the Structures of A Multi-Ligand Gated K(+) Channel. Elife V. 1 00184 2012.
PubMed: 23240087
DOI: 10.7554/ELIFE.00184