Zinc in PDB 4gbk: Crystal Structure of Aspart Insulin at pH 8.5

The structure of Crystal Structure of Aspart Insulin at pH 8.5, PDB code: 4gbk was solved by L.M.T.R.Lima, M.P.Favero-Retto, L.C.Palmieri, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.75 / 2.40
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	77.500, 77.500, 37.600, 90.00, 90.00, 120.00
R / Rfree (%)	19.8 / 26.6

The structure of Crystal Structure of Aspart Insulin at pH 8.5 also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the Crystal Structure of Aspart Insulin at pH 8.5 (pdb code 4gbk). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Aspart Insulin at pH 8.5, PDB code: 4gbk:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Aspart Insulin at pH 8.5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Aspart Insulin at pH 8.5 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn101 b:49.9 occ:0.33 NE2 B:HIS10 2.0 35.2 1.0 CL B:CL102 2.4 33.7 0.3 CE1 B:HIS10 2.8 44.7 1.0 CD2 B:HIS10 3.0 34.6 1.0 ND1 B:HIS10 4.0 40.7 1.0 CG B:HIS10 4.1 36.7 1.0 O B:LEU6 4.2 52.8 1.0 CB B:LEU6 4.9 43.3 1.0 C B:LEU6 5.0 43.9 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Aspart Insulin at pH 8.5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Aspart Insulin at pH 8.5 within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn101 b:51.0 occ:0.33 NE2 D:HIS10 2.1 35.2 1.0 CL D:CL102 2.7 58.6 0.3 CD2 D:HIS10 3.0 38.3 1.0 CE1 D:HIS10 3.2 39.7 1.0 CG D:HIS10 4.2 38.5 1.0 ND1 D:HIS10 4.3 41.3 1.0

L.C.Palmieri, M.P.Favero-Retto, D.Lourenco, L.M.Lima. A T3R3 Hexamer of the Human Insulin Variant B28ASP. Biophys.Chem. V. 173 1 2013.
ISSN: ISSN 0301-4622
PubMed: 23428413
DOI: 10.1016/J.BPC.2013.01.003