Zinc in PDB 4f8z: Carboxypeptidase T with Boc-Leu

All present enzymatic activity of Carboxypeptidase T with Boc-Leu:
3.4.17.18;

The structure of Carboxypeptidase T with Boc-Leu, PDB code: 4f8z was solved by V.I.Timofeev, S.A.Kuznetsov, V.K.Akparov, I.P.Kuranova, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.00 / 1.38
Space group	P 63 2 2
Cell size a, b, c (Å), α, β, γ (°)	157.914, 157.914, 104.068, 90.00, 90.00, 120.00
R / Rfree (%)	13.9 / 15.2

The structure of Carboxypeptidase T with Boc-Leu also contains other interesting chemical elements:

Calcium

(Ca)

4 atoms

The binding sites of Zinc atom in the Carboxypeptidase T with Boc-Leu (pdb code 4f8z). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Carboxypeptidase T with Boc-Leu, PDB code: 4f8z:

Zinc binding site 1 out of 1 in the Carboxypeptidase T with Boc-Leu


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Carboxypeptidase T with Boc-Leu within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:9.3 occ:1.00 ND1 A:HIS204 2.1 8.1 1.0 ND1 A:HIS69 2.1 8.9 1.0 O A:BL2402 2.2 18.1 1.0 OE2 A:GLU72 2.3 11.0 1.0 OE1 A:GLU72 2.3 9.9 1.0 CD A:GLU72 2.6 9.8 1.0 OXT A:BL2402 2.6 15.3 1.0 C A:BL2402 2.7 15.8 1.0 CE1 A:HIS69 3.0 9.2 1.0 CE1 A:HIS204 3.0 8.4 1.0 CG A:HIS204 3.1 7.6 1.0 CG A:HIS69 3.2 9.2 1.0 CB A:HIS204 3.5 8.1 1.0 CB A:HIS69 3.6 9.6 1.0 O A:HOH511 4.0 12.2 1.0 O A:THR205 4.0 8.8 1.0 O1 A:SO4403 4.0 17.9 0.8 CG A:GLU72 4.1 9.4 1.0 NE2 A:HIS69 4.2 9.2 1.0 NE2 A:HIS204 4.2 8.7 1.0 CA A:BL2402 4.2 18.3 1.0 O4 A:SO4403 4.2 16.2 0.8 CD2 A:HIS204 4.2 8.0 1.0 O A:HOH507 4.3 13.8 1.0 CD2 A:HIS69 4.3 9.3 1.0 NH1 A:ARG129 4.3 10.3 1.0 CA A:HIS204 4.3 7.8 1.0 O2 A:SO4403 4.4 9.2 0.8 S A:SO4403 4.5 15.5 0.8 OE2 A:GLU277 4.6 17.2 1.0 N A:THR205 4.6 8.3 1.0 N A:HIS69 4.8 9.7 1.0 CA A:HIS69 4.8 9.3 1.0 N A:BL2402 4.9 18.7 1.0 OE1 A:GLU277 5.0 13.7 1.0 CB A:GLU72 5.0 8.8 1.0 C A:HIS204 5.0 8.1 1.0

V.I.Timofeev, S.A.Kuznetsov, V.K.H.Akparov, G.G.Chestukhina, I.P.Kuranova. Three-Dimensional Structure of Carboxypeptidase T From Thermoactinomyces Vulgaris in Complex with N-Boc-L-Leucine. Biochemistry Mosc. V. 78 252 2013.
ISSN: ISSN 0006-2979
PubMed: 23586718
DOI: 10.1134/S0006297913030061