Zinc in PDB 4f4v: Human Insulin

The structure of Human Insulin, PDB code: 4f4v was solved by M.P.Favero-Retto, L.C.Palmieri, L.M.T.R.Lima, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.43 / 1.64
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	81.970, 81.970, 33.690, 90.00, 90.00, 120.00
R / Rfree (%)	20.9 / 26.4

The structure of Human Insulin also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the Human Insulin (pdb code 4f4v). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Human Insulin, PDB code: 4f4v:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Human Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Human Insulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn101 b:17.4 occ:0.33 NE2 B:HIS10 2.0 13.7 1.0 CE1 B:HIS10 2.9 14.8 1.0 CL B:CL102 2.9 45.3 0.3 CD2 B:HIS10 3.0 13.5 1.0 ND1 B:HIS10 4.0 14.2 1.0 CG B:HIS10 4.1 14.3 1.0 O D:HOH228 4.6 18.3 0.3 O B:HOH206 4.9 28.5 1.0

Zinc binding site 2 out of 2 in the Human Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Human Insulin within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn101 b:16.3 occ:0.33 NE2 D:HIS10 2.1 13.5 1.0 CL D:CL102 2.7 40.4 0.3 CE1 D:HIS10 3.1 15.9 1.0 CD2 D:HIS10 3.1 9.9 1.0 O D:HOH227 3.7 35.1 1.0 O D:HOH216 3.8 34.0 1.0 ND1 D:HIS10 4.2 12.1 1.0 CG D:HIS10 4.3 14.9 1.0 O D:HOH202 4.8 15.7 1.0

M.P.Favero-Retto, L.C.Palmieri, T.A.Souza, F.C.Almeida, L.M.Lima. Structural Meta-Analysis of Regular Human Insulin in Pharmaceutical Formulations. Eur J Pharm Biopharm V. 85 1112 2013.
PubMed: 23692694
DOI: 10.1016/J.EJPB.2013.05.005