Zinc in PDB 4c9z: Crystal Structure of SIAH1 at 1.95 A Resolution

The structure of Crystal Structure of SIAH1 at 1.95 A Resolution, PDB code: 4c9z was solved by V.Rimsa, T.C.Eadsforth, W.N.Hunter, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 1.95
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	75.140, 104.590, 133.160, 90.00, 90.00, 90.00
R / Rfree (%)	19.761 / 23.855

The structure of Crystal Structure of SIAH1 at 1.95 A Resolution also contains other interesting chemical elements:

Chlorine

(Cl)

4 atoms

The binding sites of Zinc atom in the Crystal Structure of SIAH1 at 1.95 A Resolution (pdb code 4c9z). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structure of SIAH1 at 1.95 A Resolution, PDB code: 4c9z:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Crystal Structure of SIAH1 at 1.95 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of SIAH1 at 1.95 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn283 b:41.3 occ:1.00 NE2 A:HIS147 1.9 38.9 1.0 NE2 A:HIS152 2.1 31.5 1.0 SG A:CYS128 2.2 45.4 1.0 SG A:CYS135 2.4 47.9 1.0 CE1 A:HIS152 2.7 47.6 1.0 CE1 A:HIS147 2.9 42.1 1.0 CB A:CYS128 2.9 48.3 1.0 CD2 A:HIS147 2.9 37.1 1.0 CB A:CYS135 3.2 54.8 1.0 CD2 A:HIS152 3.2 41.7 1.0 ND1 A:HIS152 3.9 43.0 1.0 ND1 A:HIS147 4.0 48.7 1.0 CG A:HIS147 4.1 38.5 1.0 CG A:HIS152 4.2 37.0 1.0 CA A:CYS128 4.3 44.5 1.0 CB A:TRP137 4.3 53.1 1.0 CA A:CYS135 4.6 62.0 1.0 SG A:CYS130 4.8 35.7 0.5 CB A:CYS130 4.9 43.7 0.5 CB A:CYS130 4.9 43.2 0.5 C A:CYS128 5.0 40.4 1.0

Zinc binding site 2 out of 4 in the Crystal Structure of SIAH1 at 1.95 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of SIAH1 at 1.95 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn284 b:53.9 occ:1.00 NE2 A:HIS117 2.0 62.4 1.0 SG A:CYS105 2.1 67.8 1.0 SG A:CYS98 2.4 49.2 1.0 SG A:CYS121 2.5 52.3 1.0 CE1 A:HIS117 3.0 70.8 1.0 CD2 A:HIS117 3.0 58.5 1.0 CB A:CYS98 3.1 50.5 1.0 CB A:CYS105 3.1 66.8 1.0 CB A:CYS121 3.3 59.8 1.0 ND1 A:HIS117 4.1 73.2 1.0 CG A:HIS117 4.1 67.2 1.0 CA A:CYS121 4.5 64.0 1.0 CA A:CYS105 4.5 70.7 1.0 CA A:CYS98 4.6 49.9 1.0 CG1 A:ILE107 4.6 71.5 1.0 CB A:TYR100 4.7 59.0 1.0 CD2 A:TYR100 4.7 56.7 1.0 CD2 A:PHE123 4.8 75.7 0.5

Zinc binding site 3 out of 4 in the Crystal Structure of SIAH1 at 1.95 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of SIAH1 at 1.95 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn283 b:40.2 occ:1.00 NE2 B:HIS152 2.0 36.0 1.0 NE2 B:HIS147 2.0 42.3 1.0 SG B:CYS128 2.1 74.5 1.0 SG B:CYS135 2.2 98.6 1.0 CE1 B:HIS152 2.9 48.1 1.0 CE1 B:HIS147 2.9 46.7 1.0 CD2 B:HIS152 2.9 38.8 1.0 CD2 B:HIS147 3.0 36.7 1.0 CB B:CYS135 3.2 0.6 1.0 CB B:CYS128 3.2 72.0 1.0 ND1 B:HIS152 3.9 39.4 1.0 CG B:HIS152 4.0 36.5 1.0 ND1 B:HIS147 4.0 42.2 1.0 CG B:HIS147 4.1 41.8 1.0 CB B:TRP137 4.5 89.6 1.0 CA B:CYS128 4.5 69.0 1.0 CA B:CYS135 4.6 0.4 1.0 CB B:GLN151 4.8 42.6 1.0

Zinc binding site 4 out of 4 in the Crystal Structure of SIAH1 at 1.95 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of SIAH1 at 1.95 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn284 b:60.5 occ:1.00 NE2 B:HIS117 2.2 77.0 1.0 SG B:CYS98 2.4 70.3 1.0 SG B:CYS121 2.5 72.5 1.0 SG B:CYS105 2.5 73.4 1.0 CB B:CYS98 3.0 70.5 1.0 CD2 B:HIS117 3.0 77.8 1.0 CE1 B:HIS117 3.2 81.4 1.0 CB B:CYS121 3.3 74.0 1.0 CB B:CYS105 3.6 72.4 1.0 CG B:HIS117 4.2 82.2 1.0 ND1 B:HIS117 4.2 84.5 1.0 CA B:CYS98 4.5 70.0 1.0 CA B:CYS121 4.5 76.3 1.0 CD2 B:TYR100 4.6 69.1 1.0 CB B:TYR100 4.8 68.1 1.0 CB B:ILE107 5.0 79.5 1.0

V.Rimsa, T.C.Eadsforth, W.N.Hunter. Two High-Resolution Structures of the Human E3 Ubiquitin Ligase SIAH1. Acta Crystallogr.,Sect.F V. 96 1339 2013.
ISSN: ISSN 1744-3091
PubMed: 24316825
DOI: 10.1107/S1744309113031448