Zinc in PDB 4a4b: Structure of Modified PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex

All present enzymatic activity of Structure of Modified PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex:
2.7.10.2; 6.3.2.19;

The structure of Structure of Modified PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex, PDB code: 4a4b was solved by H.Dou, L.Buetow, A.Hock, G.J.Sibbet, K.H.Vousden, D.T.Huang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.725 / 2.79
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	74.112, 74.112, 449.706, 90.00, 90.00, 120.00
R / Rfree (%)	18.74 / 26.68

The structure of Structure of Modified PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex also contains other interesting chemical elements:

Calcium

(Ca)

1 atom

The binding sites of Zinc atom in the Structure of Modified PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex (pdb code 4a4b). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of Modified PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex, PDB code: 4a4b:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of Modified PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Modified PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1436 b:81.1 occ:1.00 SG A:CYS381 2.2 71.3 1.0 SG A:CYS404 2.3 75.5 1.0 SG A:CYS401 2.5 83.8 1.0 SG A:CYS384 2.6 96.9 1.0 CB A:CYS401 3.0 0.7 1.0 CB A:CYS381 3.2 87.0 1.0 CB A:CYS384 3.3 85.9 1.0 CB A:CYS404 3.4 68.6 1.0 N A:CYS384 3.7 81.5 1.0 CA A:CYS401 4.0 76.5 1.0 N A:CYS401 4.0 66.3 1.0 CA A:CYS384 4.1 84.3 1.0 OD1 A:ASN387 4.3 94.2 1.0 N A:CYS404 4.3 73.0 1.0 CB A:ASN387 4.3 97.5 1.0 O A:CYS401 4.4 73.4 1.0 CA A:CYS404 4.5 68.5 1.0 C A:CYS401 4.5 76.1 1.0 CB A:ILE383 4.6 77.6 1.0 CA A:CYS381 4.6 87.0 1.0 O A:CYS381 4.7 83.2 1.0 CG A:ASN387 4.8 98.4 1.0 C A:ILE383 4.8 81.5 1.0 C A:CYS381 4.9 87.0 1.0 N A:ALA385 4.9 86.8 1.0 C A:CYS384 5.0 88.7 1.0

Zinc binding site 2 out of 2 in the Structure of Modified PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Modified PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1437 b:77.2 occ:1.00 ND1 A:HIS398 2.1 76.5 1.0 SG A:CYS416 2.2 66.0 1.0 SG A:CYS419 2.3 74.3 1.0 SG A:CYS396 2.3 69.7 1.0 CB A:CYS416 2.9 58.8 1.0 CE1 A:HIS398 3.0 73.8 1.0 CB A:CYS396 3.1 68.3 1.0 CG A:HIS398 3.1 81.0 1.0 CB A:HIS398 3.5 76.8 1.0 CB A:CYS419 3.5 85.8 1.0 N A:CYS419 3.7 92.9 1.0 NE2 A:HIS398 4.1 79.2 1.0 CA A:CYS419 4.1 86.2 1.0 CD2 A:HIS398 4.2 79.2 1.0 CB A:PHE418 4.4 67.9 1.0 CA A:CYS416 4.4 66.1 1.0 CA A:CYS396 4.5 75.0 1.0 N A:HIS398 4.6 74.2 1.0 N A:ARG420 4.6 82.5 1.0 CA A:HIS398 4.7 76.1 1.0 C A:CYS419 4.7 80.8 1.0 C A:PHE418 4.7 95.3 1.0 C A:CYS396 4.8 76.0 1.0 CA A:PHE418 4.9 80.4 1.0 N A:PHE418 4.9 81.1 1.0 C A:CYS416 4.9 69.5 1.0

H.Dou, L.Buetow, A.Hock, G.J.Sibbet, K.H.Vousden, D.T.Huang. Structural Basis For Autoinhibition and Phosphorylation-Dependent Activation of C-Cbl Nat.Struct.Mol.Biol. V. 19 184 2012.
ISSN: ISSN 1545-9993
PubMed: 22266821
DOI: 10.1038/NSMB.2231