Atomistry » Zinc » PDB 3tts-3u6m » 3tty
Atomistry »
  Zinc »
    PDB 3tts-3u6m »
      3tty »

Zinc in PDB 3tty: Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose

Enzymatic activity of Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose

All present enzymatic activity of Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose:
3.2.1.23;

Protein crystallography data

The structure of Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose, PDB code: 3tty was solved by M.Maksimainen, N.Hakulinen, J.Rouvinen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.57 / 2.25
Space group H 3
Cell size a, b, c (Å), α, β, γ (°) 228.200, 228.200, 246.800, 90.00, 90.00, 120.00
R / Rfree (%) 16 / 21

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose (pdb code 3tty). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose, PDB code: 3tty:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6;

Zinc binding site 1 out of 6 in 3tty

Go back to Zinc Binding Sites List in 3tty
Zinc binding site 1 out of 6 in the Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn676

b:17.9
occ:1.00
 SG A:CYS160 2.3 16.9 1.0
SG A:CYS155 2.4 16.7 1.0
SG A:CYS115 2.4 16.1 1.0
SG A:CYS157 2.5 17.2 1.0
CB A:CYS155 3.3 15.1 1.0
CB A:CYS160 3.3 11.8 1.0
CB A:CYS115 3.4 18.1 1.0
CB A:CYS157 3.6 17.7 1.0
N A:CYS160 3.8 15.7 1.0
N A:CYS157 4.0 12.4 1.0
CA A:CYS160 4.1 13.4 1.0
CA A:CYS115 4.2 15.8 1.0
CA A:CYS157 4.3 15.3 1.0
CD A:PRO116 4.3 16.3 1.0
C A:CYS155 4.5 16.0 1.0
CA A:CYS155 4.5 13.2 1.0
O A:HOH1003 4.5 16.7 1.0
N A:TYR156 4.7 17.3 1.0
CB A:ASN117 4.7 18.2 1.0
C A:CYS115 4.8 17.1 1.0
N A:ASN117 4.8 16.3 1.0
C A:CYS157 4.8 18.1 1.0
O A:CYS157 4.8 16.4 1.0
O A:CYS155 4.8 14.8 1.0
N A:PRO116 4.8 17.7 1.0
C A:ASN159 4.9 17.6 1.0
CB A:ASN159 5.0 16.0 1.0

Zinc binding site 2 out of 6 in 3tty

Go back to Zinc Binding Sites List in 3tty
Zinc binding site 2 out of 6 in the Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn676

b:18.9
occ:1.00
 SG B:CYS155 2.3 14.4 1.0
SG B:CYS160 2.4 15.2 1.0
SG B:CYS115 2.4 16.8 1.0
SG B:CYS157 2.6 15.4 1.0
CB B:CYS160 3.2 13.8 1.0
CB B:CYS155 3.2 14.0 1.0
CB B:CYS115 3.4 13.7 1.0
CB B:CYS157 3.7 17.5 1.0
N B:CYS160 3.8 13.5 1.0
N B:CYS157 3.9 12.2 1.0
CA B:CYS160 4.1 14.7 1.0
CA B:CYS115 4.2 16.4 1.0
CA B:CYS157 4.3 12.2 1.0
CD B:PRO116 4.4 16.6 1.0
O B:HOH744 4.4 18.6 1.0
C B:CYS155 4.4 15.0 1.0
CA B:CYS155 4.5 15.9 1.0
N B:TYR156 4.6 14.1 1.0
O B:CYS157 4.7 14.6 1.0
C B:CYS157 4.7 16.4 1.0
O B:CYS155 4.7 13.6 1.0
C B:CYS115 4.8 16.2 1.0
N B:ASN117 4.8 13.1 1.0
N B:PRO116 4.9 18.7 1.0
CB B:ASN117 4.9 16.3 1.0
C B:ASN159 4.9 13.5 1.0

Zinc binding site 3 out of 6 in 3tty

Go back to Zinc Binding Sites List in 3tty
Zinc binding site 3 out of 6 in the Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn676

b:19.6
occ:1.00
 SG C:CYS155 2.3 16.8 1.0
SG C:CYS160 2.3 14.9 1.0
SG C:CYS115 2.4 15.9 1.0
SG C:CYS157 2.7 22.0 1.0
CB C:CYS155 3.2 16.3 1.0
CB C:CYS160 3.3 12.8 1.0
CB C:CYS115 3.4 17.3 1.0
CB C:CYS157 3.7 16.5 1.0
N C:CYS160 3.9 16.3 1.0
N C:CYS157 4.0 15.6 1.0
CA C:CYS160 4.1 16.6 1.0
CA C:CYS115 4.1 17.3 1.0
CD C:PRO116 4.3 15.9 1.0
CA C:CYS157 4.4 17.7 1.0
CA C:CYS155 4.5 14.5 1.0
C C:CYS155 4.5 17.9 1.0
O C:HOH700 4.6 15.8 1.0
O C:CYS157 4.6 17.1 1.0
N C:TYR156 4.7 16.8 1.0
C C:CYS115 4.7 14.8 1.0
C C:CYS157 4.7 16.8 1.0
N C:ASN117 4.8 14.8 1.0
N C:PRO116 4.8 16.8 1.0
O C:CYS155 4.8 14.0 1.0
CB C:ASN117 4.9 14.7 1.0
C C:ASN159 4.9 17.5 1.0
CB C:ASN159 5.0 22.3 1.0

Zinc binding site 4 out of 6 in 3tty

Go back to Zinc Binding Sites List in 3tty
Zinc binding site 4 out of 6 in the Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn676

b:18.1
occ:1.00
 SG D:CYS160 2.3 15.9 1.0
SG D:CYS155 2.3 17.3 1.0
SG D:CYS115 2.4 16.8 1.0
SG D:CYS157 2.7 19.5 1.0
CB D:CYS160 3.2 12.3 1.0
CB D:CYS155 3.3 15.0 1.0
CB D:CYS115 3.4 16.6 1.0
CB D:CYS157 3.7 15.3 1.0
N D:CYS160 3.8 13.6 1.0
N D:CYS157 3.9 15.2 1.0
CA D:CYS160 4.0 13.7 1.0
CA D:CYS115 4.2 17.1 1.0
CD D:PRO116 4.3 16.6 1.0
CA D:CYS157 4.3 15.2 1.0
O D:HOH716 4.3 17.7 1.0
C D:CYS155 4.4 18.6 1.0
CA D:CYS155 4.5 14.7 1.0
N D:TYR156 4.6 15.6 1.0
O D:CYS155 4.7 16.0 1.0
C D:CYS115 4.7 15.9 1.0
N D:PRO116 4.7 17.6 1.0
N D:ASN117 4.7 16.8 1.0
C D:CYS157 4.8 19.1 1.0
O D:CYS157 4.8 18.2 1.0
C D:ASN159 4.9 17.1 1.0
CB D:ASN117 4.9 16.5 1.0

Zinc binding site 5 out of 6 in 3tty

Go back to Zinc Binding Sites List in 3tty
Zinc binding site 5 out of 6 in the Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Zn676

b:19.0
occ:1.00
 SG E:CYS155 2.3 15.5 1.0
SG E:CYS160 2.4 16.5 1.0
SG E:CYS115 2.5 18.2 1.0
SG E:CYS157 2.6 20.1 1.0
CB E:CYS155 3.2 13.7 1.0
CB E:CYS160 3.2 14.3 1.0
CB E:CYS115 3.5 16.4 1.0
CB E:CYS157 3.6 18.3 1.0
N E:CYS160 3.8 15.4 1.0
N E:CYS157 4.0 15.4 1.0
CA E:CYS160 4.0 19.7 1.0
CA E:CYS115 4.2 17.2 1.0
CA E:CYS157 4.3 15.9 1.0
C E:CYS155 4.4 17.0 1.0
CD E:PRO116 4.4 16.2 1.0
CA E:CYS155 4.4 12.8 1.0
O E:HOH731 4.5 22.7 1.0
N E:TYR156 4.6 14.8 1.0
O E:CYS155 4.7 15.6 1.0
C E:CYS157 4.7 15.9 1.0
O E:CYS157 4.7 22.1 1.0
C E:CYS115 4.8 14.5 1.0
N E:ASN117 4.8 18.4 1.0
N E:PRO116 4.9 16.9 1.0
C E:ASN159 4.9 17.9 1.0
CB E:ASN159 5.0 21.1 1.0
CB E:ASN117 5.0 20.7 1.0

Zinc binding site 6 out of 6 in 3tty

Go back to Zinc Binding Sites List in 3tty
Zinc binding site 6 out of 6 in the Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Crystal Structure of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus in Complex with Galactose within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Zn676

b:20.1
occ:1.00
 SG F:CYS155 2.3 16.3 1.0
SG F:CYS160 2.3 16.4 1.0
SG F:CYS115 2.4 16.6 1.0
SG F:CYS157 2.7 17.1 1.0
CB F:CYS160 3.2 13.8 1.0
CB F:CYS155 3.3 17.2 1.0
CB F:CYS115 3.5 17.2 1.0
CB F:CYS157 3.8 18.6 1.0
N F:CYS160 3.8 17.8 1.0
N F:CYS157 4.0 19.7 1.0
CA F:CYS160 4.1 15.9 1.0
CA F:CYS115 4.2 19.0 1.0
CD F:PRO116 4.3 13.4 1.0
CA F:CYS157 4.4 16.4 1.0
C F:CYS155 4.5 17.9 1.0
CA F:CYS155 4.5 17.1 1.0
O F:HOH706 4.7 20.2 1.0
N F:TYR156 4.7 17.8 1.0
O F:CYS155 4.7 17.3 1.0
N F:ASN117 4.8 17.9 1.0
C F:CYS115 4.8 15.1 1.0
C F:CYS157 4.8 17.7 1.0
CB F:ASN117 4.8 17.5 1.0
O F:CYS157 4.8 20.3 1.0
N F:PRO116 4.8 17.2 1.0
C F:ASN159 4.9 17.9 1.0

Reference:

M.Maksimainen, S.Paavilainen, N.Hakulinen, J.Rouvinen. Structural Analysis, Enzymatic Characterization, and Catalytic Mechanisms of Beta-Galactosidase From Bacillus Circulans Sp. Alkalophilus. Febs J. V. 279 1788 2012.
ISSN: ISSN 1742-464X
PubMed: 22385475
DOI: 10.1111/J.1742-4658.2012.08555.X
Page generated: Sat Oct 26 16:48:10 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy