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Zinc in PDB 3s15: Rna Polymerase II Initiation Complex with A 7-Nt Rna

Enzymatic activity of Rna Polymerase II Initiation Complex with A 7-Nt Rna

All present enzymatic activity of Rna Polymerase II Initiation Complex with A 7-Nt Rna:
2.7.7.6;

Protein crystallography data

The structure of Rna Polymerase II Initiation Complex with A 7-Nt Rna, PDB code: 3s15 was solved by X.Liu, D.A.Bushnell, D.A.Silva, X.Huang, R.D.Kornberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 44.11 / 3.30
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 167.928, 220.891, 194.623, 90.00, 100.16, 90.00
R / Rfree (%) 17.4 / 22.8

Other elements in 3s15:

The structure of Rna Polymerase II Initiation Complex with A 7-Nt Rna also contains other interesting chemical elements:

Magnesium (Mg) 3 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Rna Polymerase II Initiation Complex with A 7-Nt Rna (pdb code 3s15). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 8 binding sites of Zinc where determined in the Rna Polymerase II Initiation Complex with A 7-Nt Rna, PDB code: 3s15:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Zinc binding site 1 out of 8 in 3s15

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Zinc binding site 1 out of 8 in the Rna Polymerase II Initiation Complex with A 7-Nt Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Rna Polymerase II Initiation Complex with A 7-Nt Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1734

b:0.7
occ:1.00
 SG A:CYS107 2.3 0.5 1.0
SG A:CYS110 2.3 0.0 1.0
SG A:CYS148 2.3 0.1 1.0
SG A:CYS167 2.4 0.6 1.0
CB A:CYS107 3.2 0.6 1.0
CB A:CYS167 3.4 0.0 1.0
CB A:CYS148 3.5 0.3 1.0
CB A:CYS110 3.5 0.8 1.0
N A:CYS167 3.6 0.3 1.0
N A:CYS110 3.7 0.2 1.0
CA A:CYS167 4.1 0.5 1.0
CA A:CYS110 4.1 0.9 1.0
N A:GLY168 4.5 0.5 1.0
CB A:HIS109 4.5 0.4 1.0
CA A:CYS107 4.6 0.9 1.0
C A:GLY166 4.7 1.0 1.0
C A:CYS167 4.7 0.1 1.0
C A:HIS109 4.8 0.4 1.0
CA A:GLY166 4.8 0.3 1.0
CA A:CYS148 4.8 0.7 1.0
C A:CYS110 4.9 0.2 1.0
CA A:HIS109 5.0 0.0 1.0
N A:HIS109 5.0 0.3 1.0

Zinc binding site 2 out of 8 in 3s15

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Zinc binding site 2 out of 8 in the Rna Polymerase II Initiation Complex with A 7-Nt Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Rna Polymerase II Initiation Complex with A 7-Nt Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1735

b:0.7
occ:1.00
 NE2 A:HIS80 2.0 0.9 1.0
SG A:CYS67 2.3 0.4 1.0
SG A:CYS77 2.3 1.0 1.0
SG A:CYS70 2.4 0.9 1.0
CE1 A:HIS80 3.0 0.6 1.0
CD2 A:HIS80 3.1 0.7 1.0
CB A:CYS70 3.1 0.5 1.0
CB A:CYS67 3.4 0.9 1.0
CB A:CYS77 3.4 0.9 1.0
OE1 A:GLN68 3.8 0.1 1.0
ND1 A:HIS80 4.1 0.6 1.0
CG A:HIS80 4.2 0.5 1.0
CA A:CYS77 4.4 0.3 1.0
CA A:CYS70 4.6 0.8 1.0
CA A:CYS67 4.6 0.6 1.0
O A:CYS67 4.6 0.9 1.0
C A:CYS67 4.8 1.0 1.0
CD A:PRO78 4.8 0.6 1.0
CD A:GLN68 5.0 0.5 1.0

Zinc binding site 3 out of 8 in 3s15

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Zinc binding site 3 out of 8 in the Rna Polymerase II Initiation Complex with A 7-Nt Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Rna Polymerase II Initiation Complex with A 7-Nt Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1307

b:0.3
occ:1.00
 SG B:CYS1163 2.3 0.4 1.0
SG B:CYS1185 2.3 1.0 1.0
SG B:CYS1166 2.3 0.4 1.0
SG B:CYS1182 2.4 0.1 1.0
CB B:CYS1163 3.0 0.4 1.0
CB B:CYS1185 3.3 0.9 1.0
CB B:CYS1166 3.5 1.0 1.0
N B:CYS1166 3.7 0.9 1.0
CB B:CYS1182 3.7 0.6 1.0
CA B:CYS1166 4.1 0.1 1.0
CD1 A:ILE30 4.3 0.2 1.0
CB B:ILE1165 4.5 0.5 1.0
CA B:CYS1163 4.5 0.3 1.0
OG1 B:THR1170 4.6 0.3 1.0
CA B:CYS1185 4.6 0.2 1.0
C B:ILE1165 4.8 0.2 1.0
N B:CYS1185 4.9 0.6 1.0
O B:CYS1182 4.9 0.5 1.0
N B:GLY1167 4.9 0.1 1.0
C B:CYS1166 5.0 0.6 1.0
O B:LYS1183 5.0 0.9 1.0
CG2 B:ILE1165 5.0 0.6 1.0

Zinc binding site 4 out of 8 in 3s15

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Zinc binding site 4 out of 8 in the Rna Polymerase II Initiation Complex with A 7-Nt Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Rna Polymerase II Initiation Complex with A 7-Nt Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn319

b:78.7
occ:1.00
 SG C:CYS92 2.3 75.0 1.0
SG C:CYS88 2.3 69.8 1.0
SG C:CYS95 2.3 76.5 1.0
SG C:CYS86 2.3 73.0 1.0
CB C:CYS92 3.0 71.3 1.0
N C:CYS92 3.2 70.6 1.0
CB C:CYS86 3.2 68.0 1.0
CB C:CYS95 3.3 72.8 1.0
CB C:CYS88 3.4 68.1 1.0
CA C:CYS92 3.5 70.9 1.0
C C:HIS91 3.7 75.2 1.0
N C:CYS95 3.9 74.2 1.0
C C:CYS92 4.1 76.3 1.0
CA C:HIS91 4.1 72.3 1.0
CA C:CYS95 4.2 73.0 1.0
O C:CYS92 4.2 75.1 1.0
O C:HIS91 4.3 75.3 1.0
O C:ASP90 4.5 77.4 1.0
N C:CYS88 4.5 69.3 1.0
CA C:CYS88 4.6 69.0 1.0
CA C:CYS86 4.6 68.3 1.0
CB C:LYS94 4.7 79.9 1.0
N C:LYS94 4.7 77.4 1.0
C C:LYS94 4.8 79.0 1.0
N C:ASP93 4.9 75.5 1.0
CA C:LYS94 5.0 77.1 1.0
N C:HIS91 5.0 72.8 1.0
C C:CYS86 5.0 72.1 1.0

Zinc binding site 5 out of 8 in 3s15

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Zinc binding site 5 out of 8 in the Rna Polymerase II Initiation Complex with A 7-Nt Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Rna Polymerase II Initiation Complex with A 7-Nt Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Zn203

b:98.5
occ:1.00
 SG I:CYS10 2.2 0.2 1.0
SG I:CYS29 2.2 0.7 1.0
SG I:CYS32 2.4 0.8 1.0
SG I:CYS7 2.4 0.5 1.0
CB I:CYS10 2.9 0.0 1.0
CB I:CYS7 3.1 0.0 1.0
CB I:CYS32 3.4 0.1 1.0
CB I:CYS29 3.4 0.5 1.0
N I:CYS10 3.7 0.7 1.0
N I:CYS32 3.7 0.0 1.0
CA I:CYS10 3.9 0.4 1.0
CA I:CYS32 4.1 0.8 1.0
CB I:THR31 4.3 0.0 1.0
CB I:TYR34 4.5 0.2 1.0
CA I:CYS7 4.5 0.4 1.0
C I:THR31 4.6 0.8 1.0
CA I:CYS29 4.8 0.6 1.0
C I:CYS32 4.8 0.3 1.0
C I:ASP9 4.8 0.7 1.0
CA I:THR31 4.8 0.9 1.0
CB I:ASP9 4.9 0.4 1.0
N I:THR31 4.9 0.2 1.0
OG1 I:THR31 4.9 0.1 1.0

Zinc binding site 6 out of 8 in 3s15

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Zinc binding site 6 out of 8 in the Rna Polymerase II Initiation Complex with A 7-Nt Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Rna Polymerase II Initiation Complex with A 7-Nt Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Zn204

b:74.4
occ:1.00
 SG I:CYS106 2.2 72.2 1.0
SG I:CYS78 2.2 75.2 1.0
SG I:CYS103 2.3 74.0 1.0
SG I:CYS75 2.4 69.4 1.0
CB I:CYS103 3.1 69.3 1.0
CB I:CYS75 3.1 65.2 1.0
CB I:CYS106 3.4 70.2 1.0
CB I:CYS78 3.5 73.3 1.0
N I:CYS106 3.6 70.1 1.0
N I:CYS78 3.7 72.5 1.0
CA I:CYS106 4.0 69.8 1.0
CA I:CYS78 4.1 73.4 1.0
C I:CYS78 4.4 77.4 1.0
CB I:SER80 4.5 74.4 1.0
O I:CYS78 4.5 76.5 1.0
CA I:CYS103 4.5 68.1 1.0
CB I:HIS108 4.5 75.3 1.0
CB I:LYS77 4.6 71.3 1.0
CA I:CYS75 4.6 65.4 1.0
C I:CYS106 4.7 74.4 1.0
N I:SER80 4.8 73.2 1.0
C I:SER105 4.8 76.2 1.0
CB I:SER105 4.8 82.2 1.0
N I:SER107 4.8 71.9 1.0
C I:LYS77 4.8 76.2 1.0
N I:HIS108 4.9 72.4 1.0
N I:SER105 4.9 70.9 1.0

Zinc binding site 7 out of 8 in 3s15

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Zinc binding site 7 out of 8 in the Rna Polymerase II Initiation Complex with A 7-Nt Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Rna Polymerase II Initiation Complex with A 7-Nt Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
J:Zn101

b:75.4
occ:1.00
 SG J:CYS45 2.2 56.9 1.0
SG J:CYS10 2.2 58.8 1.0
SG J:CYS7 2.3 59.6 1.0
SG J:CYS46 2.4 68.5 1.0
CB J:CYS7 3.1 56.5 1.0
N J:CYS46 3.2 60.2 1.0
CB J:CYS10 3.3 54.5 1.0
CB J:CYS45 3.4 52.5 1.0
C J:CYS45 3.5 60.2 1.0
CB J:CYS46 3.6 63.8 1.0
N J:CYS10 3.6 52.1 1.0
CA J:CYS46 3.6 61.7 1.0
CA J:CYS10 4.0 53.1 1.0
NE J:ARG43 4.0 74.0 1.0
CA J:CYS45 4.0 53.1 1.0
O J:CYS45 4.1 59.6 1.0
CD J:ARG43 4.3 73.8 1.0
CG J:ARG43 4.5 73.9 1.0
CA J:CYS7 4.6 55.9 1.0
CB J:SER9 4.6 48.3 1.0
N J:GLY11 4.7 53.9 1.0
C J:SER9 4.7 54.4 1.0
N J:CYS45 4.8 54.8 1.0
C J:CYS10 4.8 56.4 1.0
O J:CYS7 4.9 57.5 1.0
CB J:LYS12 4.9 64.1 1.0
C J:CYS7 4.9 57.4 1.0
OG J:SER9 5.0 52.4 1.0
CA J:SER9 5.0 48.2 1.0
O J:ARG43 5.0 62.6 1.0

Zinc binding site 8 out of 8 in 3s15

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Zinc binding site 8 out of 8 in the Rna Polymerase II Initiation Complex with A 7-Nt Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of Rna Polymerase II Initiation Complex with A 7-Nt Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Zn105

b:0.2
occ:1.00
 SG L:CYS48 2.3 0.3 1.0
SG L:CYS34 2.3 0.0 1.0
SG L:CYS31 2.3 0.4 1.0
SG L:CYS51 2.4 0.7 1.0
CB L:CYS51 2.4 0.7 1.0
CB L:CYS48 2.9 0.1 1.0
CB L:CYS31 3.2 97.8 1.0
CB L:CYS34 3.5 0.3 1.0
CA L:CYS51 3.9 0.7 1.0
N L:CYS34 4.0 0.4 1.0
CB L:SER36 4.1 0.8 1.0
CA L:CYS34 4.3 0.8 1.0
CA L:CYS48 4.4 0.7 1.0
O L:ASP50 4.5 0.3 1.0
C L:CYS51 4.6 0.4 1.0
N L:CYS51 4.6 0.4 1.0
CA L:CYS31 4.6 96.5 1.0
CB L:GLU33 4.8 0.4 1.0
N L:GLY52 4.8 0.5 1.0
N L:SER36 4.9 0.6 1.0
C L:ASP50 4.9 0.9 1.0
C L:CYS48 4.9 0.4 1.0
C L:CYS34 5.0 0.4 1.0

Reference:

X.Liu, D.A.Bushnell, D.A.Silva, X.Huang, R.D.Kornberg. Initiation Complex Structure and Promoter Proofreading. Science V. 333 633 2011.
ISSN: ISSN 0036-8075
PubMed: 21798951
DOI: 10.1126/SCIENCE.1206629
Page generated: Sat Oct 26 15:12:25 2024

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