Zinc in PDB 3p8b: X-Ray Crystal Structure of Pyrococcus Furiosus Transcription Elongation Factor SPT4/5

The structure of X-Ray Crystal Structure of Pyrococcus Furiosus Transcription Elongation Factor SPT4/5, PDB code: 3p8b was solved by K.S.Murakami, B.J.Klein, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.80
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.532, 87.213, 133.107, 90.00, 90.00, 90.00
R / Rfree (%)	23.6 / 29.2

The binding sites of Zinc atom in the X-Ray Crystal Structure of Pyrococcus Furiosus Transcription Elongation Factor SPT4/5 (pdb code 3p8b). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the X-Ray Crystal Structure of Pyrococcus Furiosus Transcription Elongation Factor SPT4/5, PDB code: 3p8b:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the X-Ray Crystal Structure of Pyrococcus Furiosus Transcription Elongation Factor SPT4/5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of X-Ray Crystal Structure of Pyrococcus Furiosus Transcription Elongation Factor SPT4/5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn101 b:21.3 occ:1.00 SG A:CYS18 2.2 31.7 1.0 SG A:CYS6 2.3 24.2 1.0 SG A:CYS21 2.3 28.9 1.0 SG A:CYS9 2.5 24.9 1.0 CB A:CYS18 3.1 32.7 1.0 CB A:CYS6 3.3 24.5 1.0 CB A:CYS21 3.4 34.0 1.0 CB A:CYS9 3.6 23.0 1.0 N A:CYS21 3.7 33.4 1.0 N A:CYS9 3.8 25.7 1.0 CA A:CYS21 4.1 33.6 1.0 CA A:CYS9 4.2 23.4 1.0 CA A:CYS18 4.5 33.8 1.0 CB A:SER23 4.7 35.5 1.0 C A:CYS9 4.7 21.5 1.0 CA A:CYS6 4.7 23.6 1.0 CB A:HIS8 4.7 30.6 1.0 N A:HIS10 4.8 20.1 1.0 C A:CYS21 4.8 35.0 1.0 CB A:VAL20 4.8 32.5 1.0 N A:GLY22 4.8 35.5 1.0 C A:VAL20 4.8 34.2 1.0 C A:HIS8 4.8 28.1 1.0 CB A:TYR11 4.8 21.5 1.0 N A:SER23 4.9 35.5 1.0 N A:TYR11 4.9 19.7 1.0 C A:CYS18 5.0 34.2 1.0

Zinc binding site 2 out of 2 in the X-Ray Crystal Structure of Pyrococcus Furiosus Transcription Elongation Factor SPT4/5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of X-Ray Crystal Structure of Pyrococcus Furiosus Transcription Elongation Factor SPT4/5 within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn101 b:21.8 occ:1.00 SG C:CYS6 2.3 28.0 1.0 SG C:CYS9 2.4 29.7 1.0 SG C:CYS18 2.4 27.8 1.0 SG C:CYS21 2.4 32.1 1.0 CB C:CYS6 3.2 28.1 1.0 CB C:CYS18 3.2 32.4 1.0 CB C:CYS21 3.5 31.9 1.0 CB C:CYS9 3.6 26.8 1.0 N C:CYS9 3.8 27.5 1.0 N C:CYS21 3.9 31.6 1.0 CA C:CYS9 4.2 26.1 1.0 CA C:CYS21 4.2 32.0 1.0 CB C:HIS8 4.5 31.8 1.0 CA C:CYS6 4.6 27.2 1.0 CB C:VAL20 4.7 32.0 1.0 CA C:CYS18 4.7 32.0 1.0 CB C:TYR11 4.7 22.4 1.0 C C:CYS9 4.7 23.9 1.0 CB C:SER23 4.7 35.5 1.0 N C:HIS10 4.7 22.2 1.0 C C:HIS8 4.8 30.1 1.0 C C:CYS21 4.9 33.1 1.0 C C:VAL20 4.9 32.0 1.0 N C:TYR11 5.0 22.6 1.0

B.J.Klein, D.Bose, K.J.Baker, Z.M.Yusoff, X.Zhang, K.S.Murakami. Rna Polymerase and Transcription Elongation Factor SPT4/5 Complex Structure. Proc.Natl.Acad.Sci.Usa V. 108 546 2011.
ISSN: ISSN 0027-8424
PubMed: 21187417
DOI: 10.1073/PNAS.1013828108