Zinc in PDB 3p57: Crystal Structure of the P300 TAZ2 Domain Bound to MEF2 on Dna

All present enzymatic activity of Crystal Structure of the P300 TAZ2 Domain Bound to MEF2 on Dna:
2.3.1.48;

The structure of Crystal Structure of the P300 TAZ2 Domain Bound to MEF2 on Dna, PDB code: 3p57 was solved by J.He, J.Ye, C.Riquelme, J.O.Liu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.67 / 2.19
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	74.846, 90.926, 144.802, 90.00, 90.00, 90.00
R / Rfree (%)	21.9 / 27.2

The binding sites of Zinc atom in the Crystal Structure of the P300 TAZ2 Domain Bound to MEF2 on Dna (pdb code 3p57). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of the P300 TAZ2 Domain Bound to MEF2 on Dna, PDB code: 3p57:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of the P300 TAZ2 Domain Bound to MEF2 on Dna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the P300 TAZ2 Domain Bound to MEF2 on Dna within 5.0Å range: probe atom residue distance (Å) B Occ P:Zn120 b:29.4 occ:1.00 NE2 P:HIS22 2.1 24.4 1.0 SG P:CYS36 2.2 29.4 1.0 SG P:CYS31 2.4 32.3 1.0 SG P:CYS26 2.5 33.4 1.0 CD2 P:HIS22 3.0 22.3 1.0 CB P:CYS31 3.1 33.8 1.0 CB P:CYS36 3.2 29.1 1.0 CE1 P:HIS22 3.2 29.2 1.0 CB P:CYS26 3.3 32.4 1.0 NZ P:LYS40 4.1 40.4 1.0 CG P:HIS22 4.2 24.5 1.0 ND1 P:HIS22 4.3 24.1 1.0 O P:HOH130 4.4 39.0 1.0 CA P:CYS31 4.5 36.9 1.0 CA P:CYS36 4.5 31.5 1.0 CB P:LEU33 4.7 30.3 1.0 CA P:CYS26 4.7 40.0 1.0 O P:ASN28 4.9 41.4 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of the P300 TAZ2 Domain Bound to MEF2 on Dna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the P300 TAZ2 Domain Bound to MEF2 on Dna within 5.0Å range: probe atom residue distance (Å) B Occ P:Zn121 b:92.5 occ:1.00 SG P:CYS60 2.2 60.5 1.0 ND1 P:HIS45 2.7 87.8 1.0 SG P:CYS49 2.9 93.8 1.0 SG P:CYS57 2.9 0.0 1.0 CE1 P:HIS45 3.0 90.0 1.0 CB P:CYS49 3.7 0.4 1.0 N P:CYS60 3.7 72.4 1.0 CB P:CYS60 3.8 64.6 1.0 O P:PRO58 3.9 86.5 1.0 CG P:HIS45 4.0 79.3 1.0 CA P:CYS60 4.3 66.9 1.0 NE2 P:HIS45 4.3 91.7 1.0 CB P:CYS57 4.3 0.2 1.0 C P:ILE59 4.4 71.6 1.0 O P:CYS57 4.5 0.1 1.0 C P:PRO58 4.6 89.9 1.0 CA P:ILE59 4.6 77.2 1.0 CB P:HIS45 4.7 70.7 1.0 CD2 P:HIS45 4.8 80.4 1.0 C P:CYS57 4.8 0.8 1.0 N P:ILE59 5.0 87.9 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of the P300 TAZ2 Domain Bound to MEF2 on Dna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of the P300 TAZ2 Domain Bound to MEF2 on Dna within 5.0Å range: probe atom residue distance (Å) B Occ P:Zn122 b:71.8 occ:1.00 NE2 P:HIS70 2.3 55.8 1.0 SG P:CYS79 2.4 82.2 1.0 SG P:CYS74 2.4 56.9 1.0 CB P:CYS79 2.5 61.8 1.0 SG P:CYS84 2.5 56.5 1.0 CB P:CYS84 2.9 39.4 1.0 CD2 P:HIS70 2.9 45.1 1.0 CB P:CYS74 3.2 56.6 1.0 CE1 P:HIS70 3.5 48.1 1.0 CB P:VAL81 3.5 42.7 1.0 CG2 P:VAL81 3.7 42.2 1.0 CA P:CYS79 3.9 62.7 1.0 CG P:HIS70 4.2 44.6 1.0 N P:VAL81 4.3 49.5 1.0 CA P:CYS84 4.4 41.9 1.0 ND1 P:HIS70 4.4 49.2 1.0 CG1 P:VAL81 4.5 46.7 1.0 CA P:VAL81 4.5 45.7 1.0 C P:CYS79 4.5 55.9 1.0 OE2 P:GLU76 4.6 79.3 1.0 O P:VAL81 4.7 44.7 1.0 CA P:CYS74 4.7 62.1 1.0 N P:PRO80 4.9 50.3 1.0 N P:CYS79 4.9 67.7 1.0

J.He, J.Ye, Y.Cai, C.Riquelme, J.O.Liu, X.Liu, A.Han, L.Chen. Structure of P300 Bound to MEF2 on Dna Reveals A Mechanism of Enhanceosome Assembly. Nucleic Acids Res. V. 39 4464 2011.
ISSN: ISSN 0305-1048
PubMed: 21278418
DOI: 10.1093/NAR/GKR030