Zinc in PDB 3ie4: B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor

The structure of B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor, PDB code: 3ie4 was solved by Y.Mishima, F.Coste, C.Kellenberger, A.Roussel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.85 / 1.45
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	135.530, 30.680, 51.650, 90.00, 107.57, 90.00
R / Rfree (%)	16.5 / 19.8

The binding sites of Zinc atom in the B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor (pdb code 3ie4). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor, PDB code: 3ie4:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6;

Zinc binding site 1 out of 6 in the B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn200 b:3.0 occ:0.50 OE1 A:GLU43 2.0 12.3 1.0 O A:HOH187 2.0 32.4 1.0 O A:HOH175 2.0 15.8 1.0 O A:HOH188 2.4 18.7 1.0 CD A:GLU43 2.8 9.5 1.0 OE2 A:GLU43 3.0 9.9 1.0 NZ A:LYS34 4.0 14.8 1.0 O A:HOH113 4.1 10.3 1.0 CG A:GLU43 4.2 8.8 1.0 CB A:GLU43 4.7 7.6 1.0 O A:HOH189 4.7 44.4 1.0 CB A:GLU40 4.9 13.5 1.0 CE A:LYS34 5.0 16.4 1.0

Zinc binding site 2 out of 6 in the B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn201 b:4.2 occ:0.50 O A:HOH181 2.0 29.7 1.0 NE2 A:HIS32 2.0 9.3 1.0 O A:HOH176 2.2 21.1 1.0 CE1 A:HIS32 2.9 11.8 1.0 CD2 A:HIS32 3.1 12.4 1.0 O A:HOH182 3.9 30.4 1.0 ND1 A:HIS32 4.1 9.4 1.0 O A:HOH138 4.1 19.9 1.0 CG A:HIS32 4.2 8.5 1.0 CH2 A:TRP77 4.2 7.0 1.0 OD1 A:ASP50 4.4 15.6 1.0 CB A:ALA30 4.6 8.6 1.0 CZ2 A:TRP77 4.6 7.0 1.0

Zinc binding site 3 out of 6 in the B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn202 b:3.5 occ:0.50 OE1 A:GLU2 2.0 10.8 1.0 O A:HOH178 2.2 15.2 1.0 OE2 A:GLU2 2.4 13.3 1.0 CD A:GLU2 2.5 8.6 1.0 OE2 B:GLU40 3.9 21.3 1.0 CG A:GLU2 4.0 7.0 1.0 OE1 B:GLU40 4.0 23.4 1.0 CD B:GLU40 4.2 19.3 1.0 O A:HOH133 4.2 35.1 1.0 NZ A:LYS5 4.5 17.6 1.0 O B:HOH144 4.5 15.6 1.0 CB A:GLU2 4.8 7.0 1.0 O A:HOH121 4.8 16.0 1.0

Zinc binding site 4 out of 6 in the B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn108 b:3.7 occ:0.50 OE2 A:GLU24 2.0 8.8 1.0 O A:HOH170 2.1 18.4 1.0 OD2 B:ASP50 2.2 15.1 1.0 O A:HOH169 2.2 10.8 1.0 CD A:GLU24 2.8 10.8 1.0 OE1 A:GLU24 3.0 10.9 1.0 O B:HOH173 3.0 24.3 1.0 CG B:ASP50 3.2 13.8 1.0 CB B:ASP50 3.4 10.9 1.0 O B:HOH195 3.9 29.9 1.0 O B:HOH161 4.0 19.8 1.0 CG A:GLU24 4.2 10.3 1.0 OD1 B:ASP50 4.3 16.0 1.0 O B:HOH177 4.4 30.8 1.0 CA B:ASP50 4.8 10.3 1.0

Zinc binding site 5 out of 6 in the B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn200 b:4.0 occ:0.50 NE2 B:HIS32 2.0 11.6 1.0 O B:HOH180 2.2 28.9 1.0 O B:HOH171 2.4 24.0 1.0 O B:HOH179 2.5 21.5 1.0 CE1 B:HIS32 3.0 12.0 1.0 CD2 B:HIS32 3.0 11.3 1.0 O B:HOH177 4.0 30.8 1.0 ND1 B:HIS32 4.1 9.2 1.0 CG B:HIS32 4.2 9.7 1.0 O B:HOH174 4.2 32.2 1.0 CH2 B:TRP77 4.2 9.4 1.0 O B:HOH198 4.3 48.0 1.0 OD1 B:ASP50 4.3 16.0 1.0 CZ2 B:TRP77 4.5 8.9 1.0 OE2 A:GLU23 4.6 24.3 1.0 CB B:ALA30 4.8 9.6 1.0 O B:HOH173 4.9 24.3 1.0 O A:HOH209 4.9 27.1 1.0

Zinc binding site 6 out of 6 in the B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of B-Glucan Binding Domain of Drosophila GNBP3 Defines A Novel Family of Pattern Recognition Receptor within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn201 b:7.3 occ:0.50 OE1 B:GLU2 2.1 10.8 1.0 OE2 B:GLU2 2.3 10.2 1.0 O B:HOH186 2.3 25.3 1.0 CD B:GLU2 2.5 8.7 1.0 CG B:GLU2 4.0 7.0 1.0 O B:HOH120 4.3 15.3 1.0 CB B:GLU2 4.9 6.8 1.0 O B:HOH184 4.9 39.5 1.0

Y.Mishima, J.Quintin, V.Aimanianda, C.Kellenberger, F.Coste, C.Clavaud, C.Hetru, J.A.Hoffmann, J.P.Latge, D.Ferrandon, A.Roussel. The N-Terminal Domain of Drosophila Gram-Negative Binding Protein 3 (GNBP3) Defines A Novel Family of Fungal Pattern Recognition Receptors To Be Published.