Zinc in PDB 3hsv: Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2

The structure of Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2, PDB code: 3hsv was solved by M.Zhuang, B.A.Schulman, D.Miller, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.23 / 1.43
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	88.953, 43.086, 87.453, 90.00, 118.15, 90.00
R / Rfree (%)	17.4 / 20.6

The binding sites of Zinc atom in the Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2 (pdb code 3hsv). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 5 binding sites of Zinc where determined in the Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2, PDB code: 3hsv:
Jump to Zinc binding site number: 1; 2; 3; 4; 5;

Zinc binding site 1 out of 5 in the Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2 b:11.1 occ:0.50 ZN A:ZN2 0.0 11.1 0.5 ZN A:ZN2 1.7 12.2 0.5 OE2 A:GLU113 2.0 7.3 1.0 OE2 A:GLU160 2.1 9.6 1.0 O A:HOH407 2.1 8.5 0.5 CD A:GLU160 2.7 10.1 1.0 OE1 A:GLU160 2.8 9.8 1.0 CD A:GLU113 2.9 8.6 1.0 OE1 A:GLU113 3.1 11.1 1.0 CE2 A:PHE158 4.0 7.2 1.0 CG A:GLU160 4.1 8.6 1.0 NZ A:LYS103 4.1 17.6 1.0 CE A:LYS103 4.3 14.5 1.0 CG A:GLU113 4.3 8.1 1.0 CD2 A:PHE158 4.4 7.1 1.0 O A:HOH185 4.5 8.3 1.0 O A:HOH236 4.5 37.2 1.0

Zinc binding site 2 out of 5 in the Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2 b:12.2 occ:0.50 ZN A:ZN2 0.0 12.2 0.5 ZN A:ZN2 1.7 11.1 0.5 O A:HOH407 2.1 8.5 0.5 OE2 A:GLU160 2.1 9.6 1.0 OE1 A:GLU113 2.1 11.1 1.0 OE2 A:GLU113 2.4 7.3 1.0 NZ A:LYS103 2.6 17.6 1.0 CD A:GLU113 2.6 8.6 1.0 CE A:LYS103 2.7 14.5 1.0 CD A:GLU160 3.1 10.1 1.0 OE1 A:GLU160 3.3 9.8 1.0 O A:HOH185 3.7 8.3 1.0 CG A:GLU113 4.1 8.1 1.0 CD A:LYS103 4.2 10.4 1.0 CG A:GLU160 4.4 8.6 1.0 O A:HOH188 4.5 22.6 1.0 O A:HOH236 4.5 37.2 1.0 O A:HOH232 4.8 14.8 1.0 CB A:GLU113 4.9 7.3 1.0 CE2 A:PHE158 4.9 7.2 1.0 CG A:LYS103 4.9 7.0 1.0 CD2 A:PHE158 5.0 7.1 1.0

Zinc binding site 3 out of 5 in the Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn3 b:8.7 occ:0.50 ZN B:ZN3 0.0 8.7 0.5 ZN B:ZN3 1.8 11.7 0.5 OE2 B:GLU113 1.9 8.0 1.0 OE2 B:GLU160 2.2 7.5 1.0 O B:HOH408 2.2 3.5 0.5 CD B:GLU113 2.8 8.0 1.0 CD B:GLU160 2.9 6.9 1.0 OE1 B:GLU113 2.9 10.9 1.0 OE1 B:GLU160 3.1 6.7 1.0 NZ B:LYS103 3.8 16.0 0.5 NZ B:LYS103 3.9 16.3 0.5 CE2 B:PHE158 3.9 5.9 1.0 CG B:GLU113 4.2 7.5 1.0 CG B:GLU160 4.2 5.8 1.0 CD2 B:PHE158 4.3 5.3 1.0 CE B:LYS103 4.4 14.4 0.5 CE B:LYS103 4.5 14.1 0.5 O B:HOH191 4.5 7.5 1.0 O B:HOH387 4.9 31.2 1.0

Zinc binding site 4 out of 5 in the Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn3 b:11.7 occ:0.50 ZN B:ZN3 0.0 11.7 0.5 ZN B:ZN3 1.8 8.7 0.5 NZ B:LYS103 2.0 16.0 0.5 OE2 B:GLU160 2.1 7.5 1.0 O B:HOH408 2.1 3.5 0.5 OE1 B:GLU113 2.1 10.9 1.0 NZ B:LYS103 2.3 16.3 0.5 OE2 B:GLU113 2.6 8.0 1.0 CD B:GLU113 2.6 8.0 1.0 CE B:LYS103 2.8 14.4 0.5 CE B:LYS103 2.8 14.1 0.5 CD B:GLU160 3.0 6.9 1.0 OE1 B:GLU160 3.4 6.7 1.0 O B:HOH191 3.6 7.5 1.0 CG B:GLU113 4.1 7.5 1.0 CD B:LYS103 4.2 11.8 0.5 CD B:LYS103 4.2 11.8 0.5 O B:HOH262 4.3 27.0 1.0 CG B:GLU160 4.3 5.8 1.0 CB B:GLU113 4.8 6.5 1.0 CG B:LYS103 4.8 8.2 0.5 CD2 B:PHE158 4.8 5.3 1.0 CE2 B:PHE158 4.8 5.9 1.0 OG B:SER105 4.9 6.3 1.0 O B:HOH387 4.9 31.2 1.0

Zinc binding site 5 out of 5 in the Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases: Spopmathx-MACROH2ASBCPEP2 within 5.0Å range: probe atom residue distance (Å) B Occ M:Zn1 b:22.0 occ:1.00 O M:HOH308 2.0 16.0 0.5 SG A:CYS93 2.0 10.8 1.0 O M:HOH308 2.1 16.0 0.5 OD2 M:ASP180 2.3 3.0 1.0 CG M:ASP180 2.9 2.8 1.0 CB M:ASP180 3.0 2.4 1.0 CB A:CYS93 3.3 7.2 1.0 N M:GLY181 3.4 2.6 1.0 CE B:LYS134 3.5 6.4 1.0 C M:ASP180 3.8 2.2 1.0 CD2 B:LEU76 3.9 10.8 1.0 OE1 A:GLN127 3.9 7.1 1.0 CA M:ASP180 3.9 2.1 1.0 CA M:GLY181 4.0 5.1 1.0 O A:HOH177 4.0 3.7 0.5 NZ B:LYS134 4.0 5.6 1.0 OD1 M:ASP180 4.0 3.8 1.0 OH B:TYR87 4.1 7.1 1.0 C M:GLY181 4.3 7.1 1.0 CA A:CYS93 4.5 6.7 1.0 O M:ASP180 4.6 2.0 1.0 O M:GLY181 4.8 8.9 1.0 CD B:LYS134 4.9 5.7 1.0 CE1 B:TYR87 4.9 4.6 1.0 CZ B:TYR87 5.0 5.2 1.0

M.Zhuang, M.F.Calabrese, J.Liu, M.B.Waddell, A.Nourse, M.Hammel, D.J.Miller, H.Walden, D.M.Duda, S.N.Seyedin, T.Hoggard, J.W.Harper, K.P.White, B.A.Schulman. Structures of Spop-Substrate Complexes: Insights Into Molecular Architectures of Btb-CUL3 Ubiquitin Ligases. Mol.Cell V. 36 39 2009.
ISSN: ISSN 1097-2765
PubMed: 19818708
DOI: 10.1016/J.MOLCEL.2009.09.022