Zinc in PDB 3hgz: Crystal Structure of Human Insulin-Degrading Enzyme in Complex with Amylin

All present enzymatic activity of Crystal Structure of Human Insulin-Degrading Enzyme in Complex with Amylin:
3.4.24.56;

The structure of Crystal Structure of Human Insulin-Degrading Enzyme in Complex with Amylin, PDB code: 3hgz was solved by Q.Guo, Y.Bian, W.J.Tang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.69 / 2.91
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	262.868, 262.868, 90.854, 90.00, 90.00, 120.00
R / Rfree (%)	17.6 / 22.4

The binding sites of Zinc atom in the Crystal Structure of Human Insulin-Degrading Enzyme in Complex with Amylin (pdb code 3hgz). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Human Insulin-Degrading Enzyme in Complex with Amylin, PDB code: 3hgz:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Human Insulin-Degrading Enzyme in Complex with Amylin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Human Insulin-Degrading Enzyme in Complex with Amylin within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1 b:2.0 occ:1.00 O D:HOH432 1.3 28.3 1.0 OE2 A:GLU189 1.5 38.0 1.0 NE2 A:HIS108 1.8 40.4 1.0 NE2 A:HIS112 1.9 43.8 1.0 CD A:GLU189 2.4 42.9 1.0 O D:PHE15 2.5 0.1 1.0 OE1 A:GLU189 2.6 45.3 1.0 CE1 A:HIS108 2.7 41.6 1.0 CE1 A:HIS112 2.9 44.8 1.0 CD2 A:HIS112 2.9 44.0 1.0 CD2 A:HIS108 2.9 42.4 1.0 C D:PHE15 3.7 0.6 1.0 ND1 A:HIS108 3.8 41.8 1.0 CG A:GLU189 3.9 42.1 1.0 ND1 A:HIS112 4.0 44.3 1.0 CG A:HIS108 4.0 42.5 1.0 CG A:HIS112 4.0 44.1 1.0 CA D:LEU16 4.4 0.0 1.0 O D:ASN14 4.4 0.6 1.0 N D:LEU16 4.4 0.8 1.0 C D:ASN14 4.5 0.5 1.0 CB A:GLU189 4.5 43.5 1.0 CE1 A:TYR831 4.5 48.9 1.0 CB D:ASN14 4.5 0.8 1.0 N D:PHE15 4.5 0.7 1.0 OH A:TYR831 4.6 48.9 1.0 CA D:PHE15 4.7 0.8 1.0 CG2 A:VAL186 4.7 44.8 1.0 OE1 A:GLN111 4.9 44.8 1.0 NE2 A:GLN111 4.9 43.3 1.0 CZ A:TYR831 5.0 48.0 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Human Insulin-Degrading Enzyme in Complex with Amylin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Human Insulin-Degrading Enzyme in Complex with Amylin within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2 b:2.0 occ:1.00 O E:HOH334 1.3 26.1 1.0 NE2 B:HIS108 1.7 42.0 1.0 OE2 B:GLU189 1.7 50.0 1.0 NE2 B:HIS112 1.8 52.9 1.0 CE1 B:HIS108 2.3 44.1 1.0 CD B:GLU189 2.4 51.5 1.0 OE1 B:GLU189 2.5 53.3 1.0 CE1 B:HIS112 2.7 54.0 1.0 O E:PHE15 2.7 0.7 1.0 CD2 B:HIS112 2.9 53.1 1.0 CD2 B:HIS108 2.9 44.3 1.0 ND1 B:HIS108 3.5 44.9 1.0 CG B:HIS108 3.8 45.0 1.0 ND1 B:HIS112 3.8 53.2 1.0 C E:PHE15 3.9 0.2 1.0 CG B:GLU189 3.9 50.2 1.0 CG B:HIS112 3.9 52.8 1.0 O E:ASN14 4.1 0.1 1.0 C E:ASN14 4.4 0.9 1.0 CB B:GLU189 4.5 50.2 1.0 CE1 B:TYR831 4.6 56.1 1.0 N E:PHE15 4.6 0.0 1.0 N E:LEU16 4.6 0.7 1.0 CA E:LEU16 4.7 0.8 1.0 NE2 B:GLN111 4.7 47.9 1.0 CB E:ASN14 4.7 0.2 1.0 CG2 B:VAL186 4.7 51.4 1.0 CA E:PHE15 4.9 0.1 1.0 OH B:TYR831 4.9 57.9 1.0

Q.Guo, M.Manolopoulou, Y.Bian, A.B.Schilling, W.J.Tang. Molecular Basis For the Recognition and Cleavages of Igf-II, Tgf-Alpha, and Amylin By Human Insulin-Degrading Enzyme. J.Mol.Biol. V. 395 430 2010.
ISSN: ISSN 0022-2836
PubMed: 19896952
DOI: 10.1016/J.JMB.2009.10.072