Zinc in PDB 3c2s: Structural Characterization of A Human Fc Fragment Engineered For Lack of Effector Functions

The structure of Structural Characterization of A Human Fc Fragment Engineered For Lack of Effector Functions, PDB code: 3c2s was solved by V.Oganesyan, H.Wu, W.F.Dall'acqua, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.30
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	50.178, 147.301, 75.473, 90.00, 90.00, 90.00
R / Rfree (%)	21.6 / 27.5

The binding sites of Zinc atom in the Structural Characterization of A Human Fc Fragment Engineered For Lack of Effector Functions (pdb code 3c2s). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Structural Characterization of A Human Fc Fragment Engineered For Lack of Effector Functions, PDB code: 3c2s:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Structural Characterization of A Human Fc Fragment Engineered For Lack of Effector Functions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structural Characterization of A Human Fc Fragment Engineered For Lack of Effector Functions within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn448 b:37.9 occ:1.00 NE2 A:HIS310 2.0 34.9 1.0 NE2 A:HIS435 2.1 37.0 1.0 O A:HOH471 2.2 21.4 1.0 O A:HOH485 2.3 36.1 1.0 CE1 A:HIS310 2.9 34.9 1.0 CE1 A:HIS435 2.9 38.6 1.0 CD2 A:HIS310 3.0 35.3 1.0 CD2 A:HIS435 3.1 38.6 1.0 O A:HOH499 3.6 38.5 1.0 CD1 A:LEU314 3.7 35.6 1.0 ND1 A:HIS310 4.1 34.0 1.0 ND1 A:HIS435 4.1 37.4 1.0 CG A:HIS310 4.1 36.4 1.0 CG A:HIS435 4.2 37.9 1.0

Zinc binding site 2 out of 4 in the Structural Characterization of A Human Fc Fragment Engineered For Lack of Effector Functions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structural Characterization of A Human Fc Fragment Engineered For Lack of Effector Functions within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn449 b:66.1 occ:1.00 O A:HOH472 2.1 36.3 1.0 O A:HOH498 2.3 52.7 1.0 ND1 A:HIS285 2.5 44.6 1.0 CG A:HIS285 3.2 44.9 1.0 CB A:HIS285 3.2 44.7 1.0 CE1 A:HIS285 3.6 45.3 1.0 CA A:HIS285 3.7 44.3 1.0 O A:HIS285 4.3 44.6 1.0 CD2 A:HIS285 4.4 44.8 1.0 C A:HIS285 4.6 44.6 1.0 O A:VAL284 4.6 43.0 1.0 NE2 A:HIS285 4.6 43.4 1.0 N A:HIS285 4.9 43.8 1.0 OE2 A:GLU283 5.0 45.9 1.0

Zinc binding site 3 out of 4 in the Structural Characterization of A Human Fc Fragment Engineered For Lack of Effector Functions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structural Characterization of A Human Fc Fragment Engineered For Lack of Effector Functions within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn450 b:60.9 occ:0.50 OE1 A:GLU318 2.2 46.9 1.0 OE2 A:GLU318 2.3 46.7 1.0 CD A:GLU318 2.5 38.3 1.0 O A:HOH524 3.1 50.9 1.0 CG A:GLU318 4.0 41.1 1.0 CG2 A:THR335 4.6 42.0 1.0 NZ A:LYS340 4.7 42.1 1.0 CB A:GLU318 4.7 39.1 1.0

Zinc binding site 4 out of 4 in the Structural Characterization of A Human Fc Fragment Engineered For Lack of Effector Functions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Structural Characterization of A Human Fc Fragment Engineered For Lack of Effector Functions within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn451 b:63.5 occ:0.50 O A:HOH483 2.6 25.1 1.0 O A:HOH511 2.7 52.3 1.0 OE1 A:GLU345 2.7 41.6 1.0 OE2 A:GLU345 2.8 41.3 1.0 CD A:GLU345 2.9 39.2 1.0 O A:HOH482 3.6 40.0 1.0 CG A:GLU345 4.2 38.9 1.0 N A:HIS433 4.5 38.2 1.0 CB A:HIS433 4.7 39.2 1.0 CD2 A:LEU432 4.8 34.9 1.0

V.Oganesyan, C.Gao, L.Shirinian, H.Wu, W.F.Dall'acqua. Structural Characterization of A Human Fc Fragment Engineered For Lack of Effector Functions. Acta Crystallogr.,Sect.D V. 64 700 2008.
ISSN: ISSN 0907-4449
PubMed: 18560159
DOI: 10.1107/S0907444908007877