Zinc in PDB 3asn: Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State Measured at 1.7470 Angstrom Wavelength

All present enzymatic activity of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State Measured at 1.7470 Angstrom Wavelength:
1.9.3.1;

The structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State Measured at 1.7470 Angstrom Wavelength, PDB code: 3asn was solved by M.Suga, N.Yano, K.Muramoto, K.Shinzawa-Itoh, T.Maeda, E.Yamashita, T.Tsukihara, S.Yoshikawa, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 3.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	181.826, 204.098, 177.829, 90.00, 90.00, 90.00
R / Rfree (%)	15 / 18.7

The structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State Measured at 1.7470 Angstrom Wavelength also contains other interesting chemical elements:

Sodium	(Na)	2 atoms
Copper	(Cu)	6 atoms
Iron	(Fe)	4 atoms
Magnesium	(Mg)	2 atoms

The binding sites of Zinc atom in the Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State Measured at 1.7470 Angstrom Wavelength (pdb code 3asn). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State Measured at 1.7470 Angstrom Wavelength, PDB code: 3asn:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State Measured at 1.7470 Angstrom Wavelength


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State Measured at 1.7470 Angstrom Wavelength within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn99 b:22.0 occ:1.00 SG F:CYS60 2.3 23.6 1.0 SG F:CYS62 2.3 19.9 1.0 SG F:CYS82 2.4 21.9 1.0 SG F:CYS85 2.4 22.4 1.0 CB F:CYS82 3.2 20.4 1.0 CB F:CYS60 3.3 17.6 1.0 CB F:CYS85 3.4 20.3 1.0 CB F:CYS62 3.4 21.7 1.0 CA F:CYS62 3.6 22.1 1.0 N F:CYS85 3.8 20.9 1.0 N F:CYS62 4.1 21.9 1.0 CA F:CYS85 4.2 21.2 1.0 O F:CYS60 4.2 19.6 1.0 C F:CYS60 4.4 18.6 1.0 CA F:CYS60 4.5 17.9 1.0 O F:HOH2514 4.5 28.1 1.0 CA F:CYS82 4.7 19.9 1.0 OG F:SER84 4.7 22.1 1.0 CB F:SER84 4.8 20.6 1.0 C F:SER84 4.8 20.6 1.0 C F:ILE61 4.9 20.8 1.0 CG2 F:THR87 4.9 26.3 1.0 CG1 F:ILE70 4.9 15.8 1.0 C F:CYS85 4.9 22.0 1.0 C F:CYS62 4.9 23.0 1.0 CB F:ILE70 5.0 16.2 1.0

Zinc binding site 2 out of 2 in the Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State Measured at 1.7470 Angstrom Wavelength


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State Measured at 1.7470 Angstrom Wavelength within 5.0Å range: probe atom residue distance (Å) B Occ S:Zn99 b:22.7 occ:1.00 SG S:CYS62 2.3 25.3 1.0 SG S:CYS60 2.3 15.7 1.0 SG S:CYS85 2.4 19.6 1.0 SG S:CYS82 2.4 22.0 1.0 CB S:CYS82 3.2 18.0 1.0 CB S:CYS62 3.2 23.3 1.0 CB S:CYS60 3.3 16.4 1.0 CB S:CYS85 3.3 18.5 1.0 CA S:CYS62 3.6 23.9 1.0 N S:CYS85 3.7 18.3 1.0 N S:CYS62 4.1 22.5 1.0 CA S:CYS85 4.1 18.8 1.0 O S:CYS60 4.4 17.8 1.0 C S:CYS60 4.4 17.9 1.0 CA S:CYS60 4.4 16.8 1.0 OG1 S:THR87 4.6 24.9 1.0 OG S:SER84 4.6 20.2 1.0 CA S:CYS82 4.6 18.2 1.0 CB S:SER84 4.7 18.6 1.0 C S:SER84 4.7 18.4 1.0 O S:HOH3514 4.8 37.1 1.0 C S:ILE61 4.9 21.2 1.0 C S:CYS85 4.9 19.6 1.0 CB S:ILE70 5.0 16.2 1.0 C S:CYS62 5.0 25.6 1.0

M.Suga, N.Yano, K.Muramoto, K.Shinzawa-Itoh, T.Maeda, E.Yamashita, T.Tsukihara, S.Yoshikawa. Distinguishing Between Cl- and O2(2-) As the Bridging Element Between FE3+ and CU2+ in Resting-Oxidized Cytochrome C Oxidase Acta Crystallogr.,Sect.D V. 67 742 2011.
ISSN: ISSN 0907-4449
PubMed: 21795816
DOI: 10.1107/S0907444911022803