Zinc in PDB 2yxo: Histidinol Phosphate Phosphatase Complexed with Sulfate

All present enzymatic activity of Histidinol Phosphate Phosphatase Complexed with Sulfate:
3.1.3.15;

The structure of Histidinol Phosphate Phosphatase Complexed with Sulfate, PDB code: 2yxo was solved by R.Omi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.85 / 1.60
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	84.771, 97.190, 74.289, 90.00, 90.00, 90.00
R / Rfree (%)	20.2 / 22.1

The structure of Histidinol Phosphate Phosphatase Complexed with Sulfate also contains other interesting chemical elements:

Iron

(Fe)

4 atoms

The binding sites of Zinc atom in the Histidinol Phosphate Phosphatase Complexed with Sulfate (pdb code 2yxo). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Histidinol Phosphate Phosphatase Complexed with Sulfate, PDB code: 2yxo:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Histidinol Phosphate Phosphatase Complexed with Sulfate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Histidinol Phosphate Phosphatase Complexed with Sulfate within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:20.8 occ:1.00 NE2 A:HIS38 2.1 19.5 1.0 O3 A:SO42001 2.1 21.4 1.0 NE2 A:HIS13 2.2 20.9 1.0 NE2 A:HIS226 2.2 21.0 1.0 O1 A:SO42001 2.7 22.5 1.0 S A:SO42001 3.0 21.8 1.0 CD2 A:HIS13 3.1 20.9 1.0 CE1 A:HIS38 3.1 18.3 1.0 CD2 A:HIS38 3.1 18.3 1.0 CD2 A:HIS226 3.2 20.9 1.0 CE1 A:HIS13 3.2 19.7 1.0 CE1 A:HIS226 3.2 21.5 1.0 O4 A:SO42001 4.0 20.6 1.0 O2 A:SO42001 4.0 22.4 1.0 CE1 A:HIS7 4.1 18.9 1.0 NE2 A:HIS7 4.2 16.2 1.0 ND1 A:HIS38 4.2 18.0 1.0 ND1 A:HIS7 4.2 17.6 1.0 CG A:HIS38 4.2 17.5 1.0 CG A:HIS13 4.2 20.2 1.0 ND1 A:HIS13 4.3 20.2 1.0 ND1 A:HIS226 4.3 20.9 1.0 CD2 A:HIS7 4.3 18.3 1.0 CG A:HIS226 4.3 20.1 1.0 CG A:HIS7 4.4 17.5 1.0 SG A:CYS11 4.5 18.2 1.0 FE A:FE503 4.6 18.7 1.0

Zinc binding site 2 out of 2 in the Histidinol Phosphate Phosphatase Complexed with Sulfate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Histidinol Phosphate Phosphatase Complexed with Sulfate within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1501 b:20.9 occ:1.00 O2 B:SO42002 2.1 19.5 1.0 O B:HOH2132 2.2 16.9 1.0 OE2 B:GLU80 2.2 18.9 1.0 NE2 B:HIS108 2.2 18.3 1.0 NE2 B:HIS154 2.3 19.3 1.0 CE1 B:HIS108 3.0 19.5 1.0 CD2 B:HIS154 3.1 20.3 1.0 CD B:GLU80 3.1 18.1 1.0 S B:SO42002 3.2 20.4 1.0 CE1 B:HIS154 3.3 21.6 1.0 CD2 B:HIS108 3.3 19.1 1.0 OE1 B:GLU80 3.4 18.6 1.0 FE B:FE1503 3.5 17.4 1.0 O1 B:SO42002 3.5 19.6 1.0 O3 B:SO42002 3.7 20.8 1.0 ND1 B:HIS108 4.2 19.0 1.0 O B:HOH2073 4.2 26.2 1.0 OG B:SER106 4.3 18.8 1.0 CB B:SER106 4.3 18.3 1.0 CG B:HIS154 4.3 19.9 1.0 CG B:HIS108 4.3 19.5 1.0 ND1 B:HIS154 4.4 18.8 1.0 OD2 B:ASP224 4.4 18.6 1.0 CE1 B:HIS38 4.4 17.5 1.0 O4 B:SO42002 4.5 21.5 1.0 CG B:GLU80 4.5 16.7 1.0 CE1 B:HIS5 4.7 18.8 1.0 O B:HOH2070 4.8 27.8 1.0 NE2 B:HIS5 4.8 16.9 1.0 OD2 B:ASP116 4.8 24.1 1.0

R.Omi, M.Goto, I.Miyahara, M.Manzoku, A.Ebihara, K.Hirotsu. Crystal Structure of Monofunctional Histidinol Phosphate Phosphatase From Thermus Thermophilus HB8. Biochemistry V. 46 12618 2007.
ISSN: ISSN 0006-2960
PubMed: 17929834
DOI: 10.1021/BI701204R