Zinc in PDB 2xl9: Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Zn- Cuca-Closed (Semet)

The structure of Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Zn- Cuca-Closed (Semet), PDB code: 2xl9 was solved by K.J.Waldron, S.J.Firbank, S.J.Dainty, M.Perez-Rama, S.Tottey, N.J.Robinson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.11 / 2.06
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	46.750, 96.970, 114.510, 90.00, 90.00, 90.00
R / Rfree (%)	19 / 23.7

The binding sites of Zinc atom in the Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Zn- Cuca-Closed (Semet) (pdb code 2xl9). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Zn- Cuca-Closed (Semet), PDB code: 2xl9:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Zn- Cuca-Closed (Semet)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Zn- Cuca-Closed (Semet) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1269 b:29.1 occ:1.00 O A:HOH2143 2.2 29.0 1.0 NE2 A:HIS149 2.2 24.7 1.0 NE2 A:HIS90 2.2 29.0 1.0 OE1 A:GLU95 2.2 33.1 1.0 NE2 A:HIS88 2.2 27.4 1.0 O A:HOH2032 2.6 25.9 1.0 CD2 A:HIS90 3.1 26.6 1.0 CE1 A:HIS149 3.1 23.4 1.0 CD2 A:HIS88 3.1 26.2 1.0 CD2 A:HIS149 3.2 26.4 1.0 CD A:GLU95 3.2 34.2 1.0 CE1 A:HIS90 3.2 25.7 1.0 CE1 A:HIS88 3.2 24.8 1.0 OE2 A:GLU95 3.5 35.6 1.0 ND1 A:HIS149 4.2 26.8 1.0 CG A:HIS90 4.2 26.5 1.0 CG A:HIS88 4.3 26.8 1.0 ND1 A:HIS90 4.3 25.4 1.0 CG A:HIS149 4.3 25.4 1.0 ND1 A:HIS88 4.3 26.6 1.0 CZ A:PHE97 4.5 29.4 1.0 NE1 A:TRP165 4.5 33.0 1.0 CG A:GLU95 4.6 29.9 1.0 CB A:GLU95 4.9 28.9 1.0 CD1 A:TRP165 5.0 32.2 1.0 CE1 A:PHE97 5.0 31.1 1.0

Zinc binding site 2 out of 3 in the Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Zn- Cuca-Closed (Semet)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Zn- Cuca-Closed (Semet) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1270 b:38.5 occ:1.00 N A:TAM1271 2.2 42.6 1.0 NE2 A:HIS177 2.2 34.8 1.0 CE1 A:HIS177 3.1 33.5 1.0 C5 A:TAM1271 3.1 41.8 1.0 CD2 A:HIS177 3.2 33.4 1.0 C A:TAM1271 3.2 42.6 1.0 C2 A:TAM1271 3.4 42.2 1.0 C3 A:TAM1271 3.7 41.7 1.0 O A:PRO175 3.9 38.1 1.0 ND1 A:HIS177 4.3 32.6 1.0 CG A:HIS177 4.3 34.1 1.0 C1 A:TAM1271 4.5 42.9 1.0 C4 A:TAM1271 4.9 44.1 1.0

Zinc binding site 3 out of 3 in the Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Zn- Cuca-Closed (Semet)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Zn- Cuca-Closed (Semet) within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1269 b:31.8 occ:1.00 NE2 B:HIS149 2.1 25.5 1.0 OE1 B:GLU95 2.1 31.7 1.0 NE2 B:HIS90 2.1 32.9 1.0 NE2 B:HIS88 2.2 30.7 1.0 O B:HOH2032 2.7 27.3 1.0 CD2 B:HIS90 2.9 30.3 1.0 CE1 B:HIS149 3.0 26.3 1.0 CD B:GLU95 3.0 30.5 1.0 CD2 B:HIS149 3.1 27.0 1.0 CD2 B:HIS88 3.1 31.5 1.0 CE1 B:HIS88 3.2 30.1 1.0 CE1 B:HIS90 3.2 33.2 1.0 OE2 B:GLU95 3.3 30.6 1.0 ND1 B:HIS149 4.1 26.7 1.0 CG B:HIS90 4.2 30.5 1.0 CG B:HIS149 4.2 26.0 1.0 ND1 B:HIS90 4.3 32.7 1.0 ND1 B:HIS88 4.3 30.4 1.0 CG B:HIS88 4.3 28.9 1.0 NE1 B:TRP165 4.4 29.1 1.0 CG B:GLU95 4.4 29.7 1.0 CZ B:PHE97 4.5 28.3 1.0 CB B:GLU95 4.8 29.6 1.0 CD1 B:TRP165 4.9 28.5 1.0 CE1 B:PHE97 5.0 27.5 1.0

K.J.Waldron, S.J.Firbank, S.J.Dainty, M.Perez-Rama, S.Tottey, N.J.Robinson. Structure and Metal Loading of A Soluble Periplasm Cuproprotein. J.Biol.Chem. V. 285 32504 2010.
ISSN: ESSN 1083-351X
PubMed: 20702411
DOI: 10.1074/JBC.M110.153080