Zinc in PDB 2wzn: 3D Structure of TET3 From Pyrococcus Horikoshii

The structure of 3D Structure of TET3 From Pyrococcus Horikoshii, PDB code: 2wzn was solved by E.Rosenbaum, M.A.Dura, F.M.Vellieux, B.Franzetti, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.570 / 1.90
Space group	I 2 3
Cell size a, b, c (Å), α, β, γ (°)	132.241, 132.241, 132.241, 90.00, 90.00, 90.00
R / Rfree (%)	14.71 / 17.52

The structure of 3D Structure of TET3 From Pyrococcus Horikoshii also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the 3D Structure of TET3 From Pyrococcus Horikoshii (pdb code 2wzn). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the 3D Structure of TET3 From Pyrococcus Horikoshii, PDB code: 2wzn:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the 3D Structure of TET3 From Pyrococcus Horikoshii


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of 3D Structure of TET3 From Pyrococcus Horikoshii within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:33.0 occ:1.00 OD2 A:ASP185 2.0 29.9 1.0 OE2 A:GLU216 2.1 26.3 1.0 NE2 A:HIS322 2.2 26.9 1.0 CL A:CL405 2.4 54.2 1.0 OE1 A:GLU216 2.5 23.9 1.0 CD A:GLU216 2.6 26.0 1.0 O A:HOH2216 2.9 65.2 1.0 CG A:ASP185 3.0 23.0 1.0 CD2 A:HIS322 3.1 24.5 1.0 CE1 A:HIS322 3.2 33.0 1.0 OD1 A:ASP185 3.4 21.1 1.0 ZN A:ZN402 3.4 27.6 1.0 O A:HOH2135 4.1 46.7 1.0 O A:HOH2128 4.1 24.4 1.0 CG A:GLU216 4.1 23.3 1.0 CB A:ASP185 4.2 20.9 1.0 CG A:HIS322 4.3 21.8 1.0 ND1 A:HIS322 4.3 30.9 1.0 OE1 A:GLU215 4.5 29.9 1.0 CE1 A:HIS71 4.6 23.3 1.0 NE2 A:HIS71 4.6 21.5 1.0 CG2 A:ILE75 4.7 19.7 1.0 SD A:MET321 4.8 53.0 1.0 O A:HOH2137 4.8 23.5 1.0

Zinc binding site 2 out of 2 in the 3D Structure of TET3 From Pyrococcus Horikoshii


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of 3D Structure of TET3 From Pyrococcus Horikoshii within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn402 b:27.6 occ:1.00 OD1 A:ASP185 2.0 21.1 1.0 OD1 A:ASP238 2.1 25.9 1.0 NE2 A:HIS71 2.1 21.5 1.0 CL A:CL405 2.1 54.2 1.0 OD2 A:ASP238 2.7 32.5 1.0 CG A:ASP238 2.7 34.4 1.0 CG A:ASP185 3.0 23.0 1.0 CE1 A:HIS71 3.0 23.3 1.0 CD2 A:HIS71 3.2 23.2 1.0 OD2 A:ASP185 3.3 29.9 1.0 ZN A:ZN401 3.4 33.0 1.0 OE2 A:GLU216 3.8 26.3 1.0 OE1 A:GLU215 3.8 29.9 1.0 CB A:ASP186 3.8 26.5 1.0 ND1 A:HIS71 4.2 23.3 1.0 CG A:ASP186 4.2 33.7 1.0 CD A:GLU215 4.2 33.7 1.0 CB A:ASP238 4.2 25.2 1.0 CG A:HIS71 4.3 18.1 1.0 CB A:ASP185 4.4 20.9 1.0 OE2 A:GLU215 4.4 41.2 1.0 CD A:GLU216 4.4 26.0 1.0 CG2 A:VAL239 4.5 25.8 1.0 OD1 A:ASP186 4.5 25.4 1.0 CA A:ASP186 4.6 22.6 1.0 N A:VAL239 4.6 26.7 1.0 O A:HOH2216 4.6 65.2 1.0 N A:ASP186 4.7 21.2 1.0 OD2 A:ASP186 4.7 27.4 1.0 CA A:ASP185 4.7 22.8 1.0 C A:ASP185 4.7 25.0 1.0 O A:HOH2135 4.8 46.7 1.0 OE1 A:GLU216 4.8 23.9 1.0 CA A:ASP238 4.8 26.3 1.0

M.A.Dura, E.Rosenbaum, A.Larabi, F.Gabel, F.M.Vellieux, B.Franzetti. The Structural and Biochemical Characterizations of A Novel Tet Peptidase Complex From Pyrococcus Horikoshii Reveal An Integrated Peptide Degradation System in Hyperthermophilic Archaea. Mol.Microbiol. V. 72 26 2009.
ISSN: ISSN 0950-382X
PubMed: 19291145
DOI: 10.1111/J.1365-2958.2009.06600.X