Zinc in PDB 2ws7: Semi-Synthetic Analogue of Human Insulin PROB26-Dti

The structure of Semi-Synthetic Analogue of Human Insulin PROB26-Dti, PDB code: 2ws7 was solved by A.M.Brzozowski, J.Jiracek, L.Zakova, E.Antolikova, C.J.Watson, J.P.Turkenburg, G.G.Dodson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	57.26 / 2.59
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	62.451, 66.511, 57.297, 90.00, 90.00, 90.00
R / Rfree (%)	20.853 / 33.16

The structure of Semi-Synthetic Analogue of Human Insulin PROB26-Dti also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the Semi-Synthetic Analogue of Human Insulin PROB26-Dti (pdb code 2ws7). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Semi-Synthetic Analogue of Human Insulin PROB26-Dti, PDB code: 2ws7:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Semi-Synthetic Analogue of Human Insulin PROB26-Dti


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Semi-Synthetic Analogue of Human Insulin PROB26-Dti within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1020 b:18.5 occ:1.00 NE2 F:HIS10 2.0 16.3 1.0 NE2 J:HIS10 2.0 7.8 1.0 NE2 B:HIS10 2.1 13.4 1.0 CL B:CL1021 2.1 14.6 1.0 CE1 F:HIS10 2.9 16.1 1.0 CE1 J:HIS10 3.0 7.5 1.0 CD2 B:HIS10 3.0 14.1 1.0 CE1 B:HIS10 3.0 13.2 1.0 CD2 J:HIS10 3.1 7.8 1.0 CD2 F:HIS10 3.1 16.6 1.0 ND1 F:HIS10 4.1 13.1 1.0 ND1 J:HIS10 4.1 9.3 1.0 ND1 B:HIS10 4.2 12.8 1.0 CG B:HIS10 4.2 13.5 1.0 CG J:HIS10 4.2 9.6 1.0 CG F:HIS10 4.2 14.9 1.0 O J:LEU6 4.7 20.5 1.0 CB F:LEU6 4.8 18.4 1.0 O F:LEU6 4.8 19.0 1.0 CB B:LEU6 4.8 15.1 1.0 O B:LEU6 4.8 15.6 1.0 CB J:LEU6 5.0 20.2 1.0

Zinc binding site 2 out of 2 in the Semi-Synthetic Analogue of Human Insulin PROB26-Dti


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Semi-Synthetic Analogue of Human Insulin PROB26-Dti within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1024 b:16.5 occ:1.00 NE2 L:HIS10 2.0 14.2 1.0 NE2 D:HIS10 2.0 14.3 1.0 NE2 H:HIS10 2.0 9.4 1.0 CL D:CL1025 2.1 11.2 1.0 CE1 L:HIS10 2.9 13.7 1.0 CE1 H:HIS10 2.9 7.9 1.0 CE1 D:HIS10 3.0 12.0 1.0 CD2 D:HIS10 3.0 13.3 1.0 CD2 L:HIS10 3.0 13.7 1.0 CD2 H:HIS10 3.1 6.9 1.0 ND1 L:HIS10 4.0 14.0 1.0 ND1 H:HIS10 4.0 8.1 1.0 ND1 D:HIS10 4.1 13.6 1.0 CG L:HIS10 4.1 12.8 1.0 CG D:HIS10 4.1 12.2 1.0 CG H:HIS10 4.2 7.8 1.0 O H:LEU6 4.7 12.4 1.0 O D:LEU6 4.8 13.4 1.0 O L:LEU6 4.8 14.3 1.0

J.Jiracek, L.Zakova, E.Antolikova, C.J.Watson, J.P.Turkenburg, G.G.Dodson, A.M.Brzozowski. Implications For the Active Form of Human Insulin Based on the Structural Convergence of Highly Active Hormone Analogues. Proc.Natl.Acad.Sci.Usa V. 107 1966 2010.
ISSN: ISSN 0027-8424
PubMed: 20133841
DOI: 10.1073/PNAS.0911785107