Zinc in PDB 2woj: Adp-ALF4 Complex of S. Cerevisiae GET3

All present enzymatic activity of Adp-ALF4 Complex of S. Cerevisiae GET3:
3.6.3.16;

The structure of Adp-ALF4 Complex of S. Cerevisiae GET3, PDB code: 2woj was solved by A.Mateja, A.Szlachcic, M.E.Downing, M.Dobosz, M.Mariappan, R.S.Hegde, R.J.Keenan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.441 / 1.99
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	53.612, 82.628, 167.282, 90.00, 98.28, 90.00
R / Rfree (%)	17.59 / 21.27

The structure of Adp-ALF4 Complex of S. Cerevisiae GET3 also contains other interesting chemical elements:

Fluorine	(F)	16 atoms
Magnesium	(Mg)	4 atoms
Aluminium	(Al)	4 atoms

The binding sites of Zinc atom in the Adp-ALF4 Complex of S. Cerevisiae GET3 (pdb code 2woj). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Adp-ALF4 Complex of S. Cerevisiae GET3, PDB code: 2woj:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Adp-ALF4 Complex of S. Cerevisiae GET3


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Adp-ALF4 Complex of S. Cerevisiae GET3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1353 b:43.5 occ:1.00 SG B:CYS285 2.3 54.2 1.0 SG B:CYS288 2.4 45.3 1.0 SG A:CYS285 2.4 51.2 1.0 SG A:CYS288 2.4 41.3 1.0 CB B:CYS285 3.2 49.9 1.0 CB A:CYS288 3.3 48.2 1.0 CB B:CYS288 3.4 39.8 1.0 CB A:CYS285 3.5 65.7 1.0 N A:CYS285 3.7 65.1 1.0 O A:HOH2101 4.1 44.7 1.0 N A:CYS288 4.2 45.2 1.0 CA A:CYS285 4.2 62.4 1.0 N B:CYS288 4.3 44.2 1.0 CA A:CYS288 4.3 45.9 1.0 CA B:CYS288 4.4 43.7 1.0 NH2 A:ARG287 4.6 53.3 1.0 CA B:CYS285 4.7 55.6 1.0 C A:CYS285 4.8 50.6 1.0 O A:CYS285 4.9 50.2 1.0

Zinc binding site 2 out of 2 in the Adp-ALF4 Complex of S. Cerevisiae GET3


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Adp-ALF4 Complex of S. Cerevisiae GET3 within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn1352 b:40.5 occ:1.00 SG D:CYS285 2.3 51.3 1.0 SG C:CYS285 2.4 52.4 1.0 SG D:CYS288 2.4 46.5 1.0 SG C:CYS288 2.4 50.5 1.0 CB D:CYS285 3.1 49.5 1.0 CB C:CYS285 3.2 49.0 1.0 CB D:CYS288 3.3 55.9 1.0 CB C:CYS288 3.3 43.0 1.0 O C:HOH2091 4.2 39.8 1.0 N D:CYS288 4.2 44.8 1.0 N C:CYS288 4.2 44.4 1.0 O C:HOH2020 4.3 42.4 1.0 CA D:CYS288 4.3 52.1 1.0 CA C:CYS288 4.3 52.5 1.0 CA D:CYS285 4.5 51.3 1.0 CA C:CYS285 4.6 81.3 1.0 NH2 C:ARG287 4.7 56.1 1.0 O C:CYS285 4.9 50.5 1.0 O D:CYS285 4.9 49.3 1.0

A.Mateja, A.Szlachcic, M.E.Downing, M.Dobosz, M.Mariappan, R.S.Hegde, R.J.Keenan. The Structural Basis of Tail-Anchored Membrane Protein Recognition By GET3. Nature V. 461 361 2009.
ISSN: ISSN 0028-0836
PubMed: 19675567
DOI: 10.1038/NATURE08319