Zinc in PDB 2wg3: Crystal Structure of the Complex Between Human Hedgehog- Interacting Protein Hip and Desert Hedgehog Without Calcium

The structure of Crystal Structure of the Complex Between Human Hedgehog- Interacting Protein Hip and Desert Hedgehog Without Calcium, PDB code: 2wg3 was solved by B.Bishop, A.R.Aricescu, K.Harlos, C.A.O'callaghan, E.Y.Jones, C.Siebold, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.60
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	102.821, 110.701, 144.381, 90.00, 90.00, 90.00
R / Rfree (%)	22.4 / 28.1

The structure of Crystal Structure of the Complex Between Human Hedgehog- Interacting Protein Hip and Desert Hedgehog Without Calcium also contains other interesting chemical elements:

Chlorine

(Cl)

6 atoms

The binding sites of Zinc atom in the Crystal Structure of the Complex Between Human Hedgehog- Interacting Protein Hip and Desert Hedgehog Without Calcium (pdb code 2wg3). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the Complex Between Human Hedgehog- Interacting Protein Hip and Desert Hedgehog Without Calcium, PDB code: 2wg3:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of the Complex Between Human Hedgehog- Interacting Protein Hip and Desert Hedgehog Without Calcium


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Complex Between Human Hedgehog- Interacting Protein Hip and Desert Hedgehog Without Calcium within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1199 b:27.7 occ:1.00 ND1 A:HIS183 1.9 16.8 1.0 OD1 A:ASP148 2.0 18.8 1.0 OD2 C:ASP383 2.0 11.9 1.0 NE2 A:HIS141 2.1 13.9 1.0 CG A:ASP148 2.6 18.2 1.0 OD2 A:ASP148 2.7 18.8 1.0 CG C:ASP383 2.7 14.1 1.0 CE1 A:HIS183 2.7 17.5 1.0 OD1 C:ASP383 2.8 13.1 1.0 CE1 A:HIS141 2.9 15.9 1.0 CG A:HIS183 3.1 18.9 1.0 CD2 A:HIS141 3.2 13.0 1.0 CB A:HIS183 3.6 19.2 1.0 NE2 A:HIS183 3.9 17.2 1.0 CB A:ASP148 4.1 18.3 1.0 O C:HOH2018 4.1 20.4 1.0 CD2 A:HIS183 4.1 18.1 1.0 ND1 A:HIS141 4.1 15.5 1.0 CB C:ASP383 4.2 14.1 1.0 CG A:HIS141 4.3 16.0 1.0 CA A:HIS183 4.3 19.4 1.0 NE2 A:HIS181 4.4 18.7 1.0 N A:ASP148 4.5 18.1 1.0 CA A:ASP148 4.5 18.2 1.0 C A:LEU147 4.6 18.2 1.0 O A:LEU147 4.6 17.7 1.0 CD2 A:HIS181 4.8 18.5 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of the Complex Between Human Hedgehog- Interacting Protein Hip and Desert Hedgehog Without Calcium


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Complex Between Human Hedgehog- Interacting Protein Hip and Desert Hedgehog Without Calcium within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1193 b:30.9 occ:1.00 OD1 B:ASP148 2.0 19.7 1.0 ND1 B:HIS183 2.0 16.4 1.0 OD2 D:ASP383 2.1 11.6 1.0 NE2 B:HIS141 2.1 15.3 1.0 CE1 B:HIS183 2.7 17.0 1.0 CG B:ASP148 2.8 19.1 1.0 CG D:ASP383 2.8 13.8 1.0 OD2 B:ASP148 2.9 21.2 1.0 CE1 B:HIS141 2.9 16.0 1.0 OD1 D:ASP383 2.9 11.5 1.0 CG B:HIS183 3.2 18.6 1.0 CD2 B:HIS141 3.2 14.7 1.0 CB B:HIS183 3.7 18.7 1.0 NE2 B:HIS183 3.9 17.0 1.0 O B:HOH2012 4.0 21.6 1.0 ND1 B:HIS141 4.1 16.4 1.0 CD2 B:HIS183 4.1 17.6 1.0 CB B:ASP148 4.2 18.3 1.0 CB D:ASP383 4.3 14.5 1.0 CG B:HIS141 4.3 16.9 1.0 CA B:HIS183 4.5 18.8 1.0 N B:ASP148 4.5 18.3 1.0 CA B:ASP148 4.6 18.6 1.0 NE2 B:HIS181 4.6 17.2 1.0 O B:LEU147 4.6 18.2 1.0 C B:LEU147 4.7 18.0 1.0 CD2 B:HIS181 5.0 17.0 1.0

B.Bishop, A.R.Aricescu, K.Harlos, C.A.O'callaghan, E.Y.Jones, C.Siebold. Structural Insights Into Hedgehog Ligand Sequestration By the Human Hedgehog-Interacting Protein Hip Nat.Struct.Mol.Biol. V. 16 698 2009.
ISSN: ISSN 1545-9993
PubMed: 19561611
DOI: 10.1038/NSMB.1607