Zinc in PDB 2w44: Structure DELTAA1-A4 Insulin

The structure of Structure DELTAA1-A4 Insulin, PDB code: 2w44 was solved by K.S.Thorsoee, M.Schlein, J.Brandt, G.Schluckebier, H.Naver, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.34 / 2.00
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	41.050, 88.620, 73.760, 90.00, 90.00, 90.00
R / Rfree (%)	20.5 / 25.8

The structure of Structure DELTAA1-A4 Insulin also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Zinc atom in the Structure DELTAA1-A4 Insulin (pdb code 2w44). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Structure DELTAA1-A4 Insulin, PDB code: 2w44:

Zinc binding site 1 out of 1 in the Structure DELTAA1-A4 Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure DELTAA1-A4 Insulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1030 b:29.4 occ:1.00 NE2 D:HIS10 1.9 19.5 1.0 NE2 B:HIS10 2.0 20.7 1.0 NE2 F:HIS10 2.0 20.4 1.0 CL B:CL1031 2.1 38.3 1.0 CE1 D:HIS10 2.9 23.3 1.0 CD2 B:HIS10 3.0 20.2 1.0 CD2 D:HIS10 3.0 19.4 1.0 CE1 F:HIS10 3.0 22.5 1.0 CD2 F:HIS10 3.0 18.9 1.0 CE1 B:HIS10 3.0 24.8 1.0 ND1 D:HIS10 4.0 21.7 1.0 CG D:HIS10 4.1 24.0 1.0 ND1 F:HIS10 4.1 24.1 1.0 CG B:HIS10 4.1 21.4 1.0 ND1 B:HIS10 4.1 22.6 1.0 CG F:HIS10 4.1 23.9 1.0 O F:LEU6 4.7 24.5 1.0 O D:LEU6 4.7 26.5 1.0 O B:LEU6 4.7 22.4 1.0 CB F:LEU6 4.8 22.8 1.0 CB D:LEU6 4.9 24.8 1.0 O F:HOH2002 4.9 48.3 1.0

K.S.Thorsoe, M.Schlein, D.B.Steensgaard, J.Brandt, G.Schluckebier, H.Naver. Kinetic Evidence For the Sequential Association of Insulin Binding Sites 1 and 2 to the Insulin Receptor and the Influence of Receptor Isoform. Biochemistry V. 49 6234 2010.
ISSN: ISSN 0006-2960
PubMed: 20568733
DOI: 10.1021/BI1000118