Zinc in PDB 2vh9: Crystal Structure of NXG1-Deltayniig in Complex with Xllg, A Xyloglucan Derived Oligosaccharide

All present enzymatic activity of Crystal Structure of NXG1-Deltayniig in Complex with Xllg, A Xyloglucan Derived Oligosaccharide:
3.2.1.151; 3.2.1.4;

The structure of Crystal Structure of NXG1-Deltayniig in Complex with Xllg, A Xyloglucan Derived Oligosaccharide, PDB code: 2vh9 was solved by M.Czjzek, P.Mark, M.J.Baumann, J.M.Eklof, G.Michel, H.Brumer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.00 / 2.10
Space group	P 31
Cell size a, b, c (Å), α, β, γ (°)	100.540, 100.540, 61.130, 90.00, 90.00, 120.00
R / Rfree (%)	14.8 / 20.3

The binding sites of Zinc atom in the Crystal Structure of NXG1-Deltayniig in Complex with Xllg, A Xyloglucan Derived Oligosaccharide (pdb code 2vh9). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of NXG1-Deltayniig in Complex with Xllg, A Xyloglucan Derived Oligosaccharide, PDB code: 2vh9:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of NXG1-Deltayniig in Complex with Xllg, A Xyloglucan Derived Oligosaccharide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of NXG1-Deltayniig in Complex with Xllg, A Xyloglucan Derived Oligosaccharide within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn301 b:71.9 occ:1.00 OD1 A:ASP259 2.3 15.6 1.0 OG1 A:THR261 2.5 18.4 1.0 CG A:ASP259 3.1 17.8 1.0 CB A:THR261 3.4 17.0 1.0 OD2 A:ASP259 3.5 18.9 1.0 N A:THR261 4.1 16.5 1.0 CA A:THR261 4.3 16.7 1.0 CB A:ASP259 4.4 16.7 1.0 CG2 A:THR261 4.4 17.5 1.0 O A:HOH646 4.5 24.0 1.0 CA A:ASP259 4.7 16.6 1.0 N A:HIS260 4.7 15.7 1.0 C A:ASP259 4.8 16.1 1.0 O A:HOH469 4.8 16.9 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of NXG1-Deltayniig in Complex with Xllg, A Xyloglucan Derived Oligosaccharide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of NXG1-Deltayniig in Complex with Xllg, A Xyloglucan Derived Oligosaccharide within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn302 b:65.8 occ:1.00 OD1 B:ASP259 2.2 16.6 1.0 OG1 B:THR261 2.5 18.9 1.0 CG B:ASP259 3.2 17.9 1.0 CB B:THR261 3.3 17.2 1.0 OD2 B:ASP259 3.6 18.9 1.0 N B:THR261 4.1 16.4 1.0 CA B:THR261 4.3 16.9 1.0 CG2 B:THR261 4.3 17.6 1.0 CB B:ASP259 4.5 17.2 1.0 O B:HOH649 4.6 21.7 1.0 CA B:ASP259 4.8 17.0 1.0 O B:HOH439 4.8 13.2 1.0 N B:HIS260 4.8 16.0 1.0 C B:ASP259 4.9 16.4 1.0

P.Mark, M.J.Baumann, J.M.Eklof, F.Gullfot, G.Michel, A.M.Kallas, T.T.Teeri, H.Brumer, M.Czjzek. Analysis of Nasturtium TMNXG1 Complexes By Crystallography and Molecular Dynamics Provides Detailed Insight Into Substrate Recognition By Family GH16 Xyloglucan Endo-Transglycosylases and Endo-Hydrolases. Proteins V. 75 820 2009.
ISSN: ISSN 0887-3585
PubMed: 19004021
DOI: 10.1002/PROT.22291