Zinc in PDB 2uzi: Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex

The structure of Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex, PDB code: 2uzi was solved by T.Tanaka, R.L.Williams, T.H.Rabbitts, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.89 / 2.00
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	75.528, 84.632, 62.592, 90.00, 90.00, 90.00
R / Rfree (%)	19.4 / 27.1

The structure of Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex (pdb code 2uzi). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 5 binding sites of Zinc where determined in the Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex, PDB code: 2uzi:
Jump to Zinc binding site number: 1; 2; 3; 4; 5;

Zinc binding site 1 out of 5 in the Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex within 5.0Å range: probe atom residue distance (Å) B Occ H:Zn1115 b:67.8 occ:1.00 OD1 H:ASP73 2.3 22.6 1.0 OG H:SER75 2.5 26.2 1.0 O H:HOH2106 2.5 41.9 1.0 O H:HOH2071 2.8 37.6 1.0 CG H:ASP73 3.0 21.5 1.0 OD2 H:ASP73 3.2 23.5 1.0 CB H:SER75 3.4 22.4 1.0 N H:SER75 4.0 20.8 1.0 CA H:SER75 4.2 22.9 1.0 CB H:ASP73 4.3 18.0 1.0 O H:HOH2075 4.4 38.9 1.0 CA H:ASP73 4.6 16.9 1.0 N H:LYS76 4.6 22.6 1.0 C H:ASP73 4.7 16.9 1.0 N H:ASN74 4.7 17.5 1.0 CG H:LYS76 4.8 24.9 1.0 C H:SER75 4.8 23.1 1.0

Zinc binding site 2 out of 5 in the Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex within 5.0Å range: probe atom residue distance (Å) B Occ H:Zn1116 b:50.8 occ:1.00 OD2 H:ASP62 2.2 26.8 1.0 OD1 H:ASP62 2.8 29.5 1.0 CG H:ASP62 2.8 24.1 1.0 O H:HOH2107 3.1 44.9 1.0 CB H:ASP62 4.3 21.2 1.0 O H:HOH2060 4.8 33.9 1.0 NZ H:LYS65 4.8 34.3 1.0 CD L:PRO97 4.8 24.1 1.0

Zinc binding site 3 out of 5 in the Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex within 5.0Å range: probe atom residue distance (Å) B Occ R:Zn1169 b:29.3 occ:1.00 O R:HOH2127 2.3 31.5 1.0 CD2 R:HIS27 2.3 27.6 1.0 O R:HOH2128 2.9 45.3 1.0 CG R:HIS27 3.1 26.6 1.0 CB R:HIS27 3.4 22.1 1.0 NE2 R:HIS27 3.5 30.4 1.0 ND1 R:HIS27 4.4 29.3 1.0 CA R:HIS27 4.4 21.6 1.0 CE1 R:HIS27 4.5 27.1 1.0 OG1 H:THR28 4.6 15.8 1.0 CG2 H:THR28 4.8 13.5 1.0

Zinc binding site 4 out of 5 in the Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex within 5.0Å range: probe atom residue distance (Å) B Occ R:Zn1170 b:89.8 occ:1.00 O R:HIS166 2.8 30.1 1.0 C R:HIS166 2.9 26.5 1.0 O R:HOH2123 3.9 32.2 1.0 CA R:HIS166 4.3 26.0 1.0 O R:ILE163 4.4 21.6 1.0 O R:ARG164 4.5 23.9 1.0 N R:HIS166 4.7 26.0 1.0

Zinc binding site 5 out of 5 in the Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Crystal Structure of Hras(G12V) - Anti-Ras Fv Complex within 5.0Å range: probe atom residue distance (Å) B Occ R:Zn1171 b:27.2 occ:1.00 OD1 R:ASP108 2.0 27.7 1.0 NE2 R:HIS166 2.1 20.0 1.0 CG R:ASP108 2.7 27.5 1.0 OD2 R:ASP108 2.8 29.6 1.0 CD2 R:HIS166 3.0 19.9 1.0 CE1 R:HIS166 3.1 20.1 1.0 O R:HOH2121 4.1 21.6 1.0 CG R:HIS166 4.1 23.7 1.0 CB R:ASP108 4.1 25.8 1.0 ND1 R:HIS166 4.2 21.4 1.0 O R:GLY138 4.2 15.9 1.0 O R:HOH2089 4.3 26.0 1.0 O R:HOH2122 4.5 15.5 1.0 O R:ASP108 4.6 24.1 1.0 C R:ASP108 4.6 23.7 1.0 CA R:ASP108 4.6 25.2 1.0 OE1 R:GLU162 4.8 24.7 1.0 O R:VAL109 4.8 18.0 1.0

T.Tanaka, R.L.Williams, T.H.Rabbitts. Tumour Prevention By A Single Antibody Domain Targeting the Interaction of Signal Transduction Proteins with Ras. Embo J. V. 26 3250 2007.
ISSN: ISSN 0261-4189
PubMed: 17568777
DOI: 10.1038/SJ.EMBOJ.7601744